Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography

Autores
Flor, Sabrina Andrea; Tripodi, Valeria Paula; Contin, Mario Daniel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heparin (Hep) is a linear sulfated polysaccharide used in clinical treatment of tromboembolic diseases. In 2008, contaminated batches of Hep appeared in the pharmaceutical market. The contaminant was identified as an over sulfated form of chondroitin sulfate (oversulfated chondroitin sulfate, OSCS). Different analytical methods have been developed to identify and quantify OSCS and other impurities expressed as dermatan sulfate from Hep. In this sense, we have developed a capillary electrophoretic method based on the use of a mixture of polymers such as polymeric b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacobótanica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Materia
HEPARIN AND RELATED POLYSACCHAIDES
POLYMERIC B CYCLODEXTRIN
SPECTROSCOPIC TECHNIQUES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271570
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatographyFlor, Sabrina AndreaTripodi, Valeria PaulaContin, Mario DanielDobrecky, Cecilia BeatrizLucangioli, Silvia EdithHEPARIN AND RELATED POLYSACCHAIDESPOLYMERIC B CYCLODEXTRINSPECTROSCOPIC TECHNIQUEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Heparin (Hep) is a linear sulfated polysaccharide used in clinical treatment of tromboembolic diseases. In 2008, contaminated batches of Hep appeared in the pharmaceutical market. The contaminant was identified as an over sulfated form of chondroitin sulfate (oversulfated chondroitin sulfate, OSCS). Different analytical methods have been developed to identify and quantify OSCS and other impurities expressed as dermatan sulfate from Hep. In this sense, we have developed a capillary electrophoretic method based on the use of a mixture of polymers such as polymeric b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacobótanica; ArgentinaFil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaJournal of Chemical and Pharmaceutical Research2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271570Flor, Sabrina Andrea; Tripodi, Valeria Paula; Contin, Mario Daniel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography; Journal of Chemical and Pharmaceutical Research; Journal of Chemical and Pharmaceutical Research; 4; 2; 2-2012; 972-9790975-7384CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jocpr.com/vol4-iss2-2012/JCPR-2012-4-2-972-979.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:40:15Zoai:ri.conicet.gov.ar:11336/271570instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:40:15.863CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
title Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
spellingShingle Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
Flor, Sabrina Andrea
HEPARIN AND RELATED POLYSACCHAIDES
POLYMERIC B CYCLODEXTRIN
SPECTROSCOPIC TECHNIQUES
title_short Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
title_full Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
title_fullStr Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
title_full_unstemmed Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
title_sort Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Tripodi, Valeria Paula
Contin, Mario Daniel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Tripodi, Valeria Paula
Contin, Mario Daniel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
author_role author
author2 Tripodi, Valeria Paula
Contin, Mario Daniel
Dobrecky, Cecilia Beatriz
Lucangioli, Silvia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv HEPARIN AND RELATED POLYSACCHAIDES
POLYMERIC B CYCLODEXTRIN
SPECTROSCOPIC TECHNIQUES
topic HEPARIN AND RELATED POLYSACCHAIDES
POLYMERIC B CYCLODEXTRIN
SPECTROSCOPIC TECHNIQUES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Heparin (Hep) is a linear sulfated polysaccharide used in clinical treatment of tromboembolic diseases. In 2008, contaminated batches of Hep appeared in the pharmaceutical market. The contaminant was identified as an over sulfated form of chondroitin sulfate (oversulfated chondroitin sulfate, OSCS). Different analytical methods have been developed to identify and quantify OSCS and other impurities expressed as dermatan sulfate from Hep. In this sense, we have developed a capillary electrophoretic method based on the use of a mixture of polymers such as polymeric b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Tripodi, Valeria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Contin, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Dobrecky, Cecilia Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacobótanica; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
description Heparin (Hep) is a linear sulfated polysaccharide used in clinical treatment of tromboembolic diseases. In 2008, contaminated batches of Hep appeared in the pharmaceutical market. The contaminant was identified as an over sulfated form of chondroitin sulfate (oversulfated chondroitin sulfate, OSCS). Different analytical methods have been developed to identify and quantify OSCS and other impurities expressed as dermatan sulfate from Hep. In this sense, we have developed a capillary electrophoretic method based on the use of a mixture of polymers such as polymeric b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.b-cyclodextrin and poloxamine. As a result of this combination, not only the resolution of Hep from its impurities and contaminant was obtained but the sensitivity was extremely improved. The association of Hep and its related polysaccharides with the polymers was characterized by spectroscopic techniques as ultraviolet (UV) and infrared (IR). The results suggest that the interaction between polysaccharides and polymers would principally be through the sulfate groups of polysaccharides.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271570
Flor, Sabrina Andrea; Tripodi, Valeria Paula; Contin, Mario Daniel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography; Journal of Chemical and Pharmaceutical Research; Journal of Chemical and Pharmaceutical Research; 4; 2; 2-2012; 972-979
0975-7384
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271570
identifier_str_mv Flor, Sabrina Andrea; Tripodi, Valeria Paula; Contin, Mario Daniel; Dobrecky, Cecilia Beatriz; Lucangioli, Silvia Edith; Spectroscopic approach of the association of heparin and its contaminant and related polysaccharides with polymers used in electrokinetic chromatography; Journal of Chemical and Pharmaceutical Research; Journal of Chemical and Pharmaceutical Research; 4; 2; 2-2012; 972-979
0975-7384
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jocpr.com/vol4-iss2-2012/JCPR-2012-4-2-972-979.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Journal of Chemical and Pharmaceutical Research
publisher.none.fl_str_mv Journal of Chemical and Pharmaceutical Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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