Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in fe...

Autores
McCabe, Matthew Sean; Cormican, Paul; Keogh, Kate; O'Connor, Aaron; Ohara, Eoin; Palladino, Rafael Alejandro; Kenny, David Anthony; Waters, Sinéad Mary
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Periodic feed restriction is used in cattle production to reduce feed costs. When normal feed levels are resumed, cattle catch up to a normal weight by an acceleration of normal growth rate, known as compensatory growth, which is not yet fully understood. Illumina Miseq Phylogenetic marker amplicon sequencing of DNA extracted from rumen contents of 55 bulls showed that restriction of feed (70% concentrate, 30% grass silage) for 125 days, to levels that caused a 60% reduction of growth rate, resulted in a large increase of relative abundance of Methanobrevibacter gottschalkii clade (designated as OTU-M7), and a large reduction of an uncharacterised Succinivibrionaceae species (designated as OTU-S3004). There was a strong negative Spearman correlation (ρ = -0.72, P = <1x10-20) between relative abundances of OTU-3004 and OTU-M7 in the liquid rumen fraction. There was also a significant increase in acetate:propionate ratio (A:P) in feed restricted animals that showed a negative Spearman correlation (ρ = -0.69, P = <1x10-20) with the relative abundance of OTU-S3004 in the rumen liquid fraction but not the solid fraction, and a strong positive Spearman correlation with OTU-M7 in the rumen liquid (ρ = 0.74, P = <1x10-20) and solid (ρ = 0.69, P = <1x10-20) fractions. Reduced A:P ratios in the rumen are associated with increased feed efficiency and reduced production of methane which has a global warming potential (GWP 100 years) of 28. Succinivibrionaceae growth in the rumen was previously suggested to reduce methane emissions as some members of this family utilise hydrogen, which is also utilised by methanogens for methanogenesis, to generate succinate which is converted to propionate. Relative abundance of OTU-S3004 showed a positive Spearman correlation with propionate (ρ = 0.41, P = <0.01) but not acetate in the liquid rumen fraction.
Fil: McCabe, Matthew Sean. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Cormican, Paul. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Keogh, Kate. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: O'Connor, Aaron. University College Dublin; Irlanda
Fil: Ohara, Eoin. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kenny, David Anthony. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Waters, Sinéad Mary. Animal and Grassland Research and Innovation Centre; Irlanda
Materia
SUCCINIVIBRIONACEAE
METHANOBREVIBACTER GOTTSCHALKII
METHANE
16S rDNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16790
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16790
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattleMcCabe, Matthew SeanCormican, PaulKeogh, KateO'Connor, AaronOhara, EoinPalladino, Rafael AlejandroKenny, David AnthonyWaters, Sinéad MarySUCCINIVIBRIONACEAEMETHANOBREVIBACTER GOTTSCHALKIIMETHANE16S rDNAhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Periodic feed restriction is used in cattle production to reduce feed costs. When normal feed levels are resumed, cattle catch up to a normal weight by an acceleration of normal growth rate, known as compensatory growth, which is not yet fully understood. Illumina Miseq Phylogenetic marker amplicon sequencing of DNA extracted from rumen contents of 55 bulls showed that restriction of feed (70% concentrate, 30% grass silage) for 125 days, to levels that caused a 60% reduction of growth rate, resulted in a large increase of relative abundance of Methanobrevibacter gottschalkii clade (designated as OTU-M7), and a large reduction of an uncharacterised Succinivibrionaceae species (designated as OTU-S3004). There was a strong negative Spearman correlation (ρ = -0.72, P = <1x10-20) between relative abundances of OTU-3004 and OTU-M7 in the liquid rumen fraction. There was also a significant increase in acetate:propionate ratio (A:P) in feed restricted animals that showed a negative Spearman correlation (ρ = -0.69, P = <1x10-20) with the relative abundance of OTU-S3004 in the rumen liquid fraction but not the solid fraction, and a strong positive Spearman correlation with OTU-M7 in the rumen liquid (ρ = 0.74, P = <1x10-20) and solid (ρ = 0.69, P = <1x10-20) fractions. Reduced A:P ratios in the rumen are associated with increased feed efficiency and reduced production of methane which has a global warming potential (GWP 100 years) of 28. Succinivibrionaceae growth in the rumen was previously suggested to reduce methane emissions as some members of this family utilise hydrogen, which is also utilised by methanogens for methanogenesis, to generate succinate which is converted to propionate. Relative abundance of OTU-S3004 showed a positive Spearman correlation with propionate (ρ = 0.41, P = <0.01) but not acetate in the liquid rumen fraction.Fil: McCabe, Matthew Sean. Animal and Grassland Research and Innovation Centre; IrlandaFil: Cormican, Paul. Animal and Grassland Research and Innovation Centre; IrlandaFil: Keogh, Kate. Animal and Grassland Research and Innovation Centre; IrlandaFil: O'Connor, Aaron. University College Dublin; IrlandaFil: Ohara, Eoin. Animal and Grassland Research and Innovation Centre; IrlandaFil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kenny, David Anthony. Animal and Grassland Research and Innovation Centre; IrlandaFil: Waters, Sinéad Mary. Animal and Grassland Research and Innovation Centre; IrlandaPublic Library Of Science2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16790McCabe, Matthew Sean; Cormican, Paul; Keogh, Kate; O'Connor, Aaron; Ohara, Eoin; et al.; Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle; Public Library Of Science; Plos One; 2015; 10; 7-2015; 1-25; e01332341932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133234info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133234info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:40Zoai:ri.conicet.gov.ar:11336/16790instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:41.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
title Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
spellingShingle Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
McCabe, Matthew Sean
SUCCINIVIBRIONACEAE
METHANOBREVIBACTER GOTTSCHALKII
METHANE
16S rDNA
title_short Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
title_full Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
title_fullStr Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
title_full_unstemmed Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
title_sort Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle
dc.creator.none.fl_str_mv McCabe, Matthew Sean
Cormican, Paul
Keogh, Kate
O'Connor, Aaron
Ohara, Eoin
Palladino, Rafael Alejandro
Kenny, David Anthony
Waters, Sinéad Mary
author McCabe, Matthew Sean
author_facet McCabe, Matthew Sean
Cormican, Paul
Keogh, Kate
O'Connor, Aaron
Ohara, Eoin
Palladino, Rafael Alejandro
Kenny, David Anthony
Waters, Sinéad Mary
author_role author
author2 Cormican, Paul
Keogh, Kate
O'Connor, Aaron
Ohara, Eoin
Palladino, Rafael Alejandro
Kenny, David Anthony
Waters, Sinéad Mary
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SUCCINIVIBRIONACEAE
METHANOBREVIBACTER GOTTSCHALKII
METHANE
16S rDNA
topic SUCCINIVIBRIONACEAE
METHANOBREVIBACTER GOTTSCHALKII
METHANE
16S rDNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Periodic feed restriction is used in cattle production to reduce feed costs. When normal feed levels are resumed, cattle catch up to a normal weight by an acceleration of normal growth rate, known as compensatory growth, which is not yet fully understood. Illumina Miseq Phylogenetic marker amplicon sequencing of DNA extracted from rumen contents of 55 bulls showed that restriction of feed (70% concentrate, 30% grass silage) for 125 days, to levels that caused a 60% reduction of growth rate, resulted in a large increase of relative abundance of Methanobrevibacter gottschalkii clade (designated as OTU-M7), and a large reduction of an uncharacterised Succinivibrionaceae species (designated as OTU-S3004). There was a strong negative Spearman correlation (ρ = -0.72, P = <1x10-20) between relative abundances of OTU-3004 and OTU-M7 in the liquid rumen fraction. There was also a significant increase in acetate:propionate ratio (A:P) in feed restricted animals that showed a negative Spearman correlation (ρ = -0.69, P = <1x10-20) with the relative abundance of OTU-S3004 in the rumen liquid fraction but not the solid fraction, and a strong positive Spearman correlation with OTU-M7 in the rumen liquid (ρ = 0.74, P = <1x10-20) and solid (ρ = 0.69, P = <1x10-20) fractions. Reduced A:P ratios in the rumen are associated with increased feed efficiency and reduced production of methane which has a global warming potential (GWP 100 years) of 28. Succinivibrionaceae growth in the rumen was previously suggested to reduce methane emissions as some members of this family utilise hydrogen, which is also utilised by methanogens for methanogenesis, to generate succinate which is converted to propionate. Relative abundance of OTU-S3004 showed a positive Spearman correlation with propionate (ρ = 0.41, P = <0.01) but not acetate in the liquid rumen fraction.
Fil: McCabe, Matthew Sean. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Cormican, Paul. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Keogh, Kate. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: O'Connor, Aaron. University College Dublin; Irlanda
Fil: Ohara, Eoin. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kenny, David Anthony. Animal and Grassland Research and Innovation Centre; Irlanda
Fil: Waters, Sinéad Mary. Animal and Grassland Research and Innovation Centre; Irlanda
description Periodic feed restriction is used in cattle production to reduce feed costs. When normal feed levels are resumed, cattle catch up to a normal weight by an acceleration of normal growth rate, known as compensatory growth, which is not yet fully understood. Illumina Miseq Phylogenetic marker amplicon sequencing of DNA extracted from rumen contents of 55 bulls showed that restriction of feed (70% concentrate, 30% grass silage) for 125 days, to levels that caused a 60% reduction of growth rate, resulted in a large increase of relative abundance of Methanobrevibacter gottschalkii clade (designated as OTU-M7), and a large reduction of an uncharacterised Succinivibrionaceae species (designated as OTU-S3004). There was a strong negative Spearman correlation (ρ = -0.72, P = <1x10-20) between relative abundances of OTU-3004 and OTU-M7 in the liquid rumen fraction. There was also a significant increase in acetate:propionate ratio (A:P) in feed restricted animals that showed a negative Spearman correlation (ρ = -0.69, P = <1x10-20) with the relative abundance of OTU-S3004 in the rumen liquid fraction but not the solid fraction, and a strong positive Spearman correlation with OTU-M7 in the rumen liquid (ρ = 0.74, P = <1x10-20) and solid (ρ = 0.69, P = <1x10-20) fractions. Reduced A:P ratios in the rumen are associated with increased feed efficiency and reduced production of methane which has a global warming potential (GWP 100 years) of 28. Succinivibrionaceae growth in the rumen was previously suggested to reduce methane emissions as some members of this family utilise hydrogen, which is also utilised by methanogens for methanogenesis, to generate succinate which is converted to propionate. Relative abundance of OTU-S3004 showed a positive Spearman correlation with propionate (ρ = 0.41, P = <0.01) but not acetate in the liquid rumen fraction.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16790
McCabe, Matthew Sean; Cormican, Paul; Keogh, Kate; O'Connor, Aaron; Ohara, Eoin; et al.; Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle; Public Library Of Science; Plos One; 2015; 10; 7-2015; 1-25; e0133234
1932-6203
url http://hdl.handle.net/11336/16790
identifier_str_mv McCabe, Matthew Sean; Cormican, Paul; Keogh, Kate; O'Connor, Aaron; Ohara, Eoin; et al.; Illumina MiSeq Phylogenetic amplicon sequencing shows a large reduction of an uncharacterised Succinivibrionaceae and an increase of the Methanobrevibacter Gottschalkii clade in feed restricted cattle; Public Library Of Science; Plos One; 2015; 10; 7-2015; 1-25; e0133234
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133234
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133234
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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