Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process

Autores
Pizzolitto, Romina Paola; Salvano, Mario Armando; Dalcero, Ana Maria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this investigation were to evaluate the ability of Saccharomyces cerevisiae CECT 1891 and Lactobacillus acidophilus 24 to remove fumonisin B1 (FB1) from liquid medium; to determine the nature of the mechanism involved in FB1?microorganism interaction and to analyze whether the presence of aflatoxin B1 (AFB1) interferes with the removal of FB1 and vice versa. The results obtained indicated that: (i) both microorganisms were able to remove FB1 from liquid medium; (ii) the removal was a fast and reversible process; (iii) cell viability was not necessary; (iv) the amount of FB1 removed was both toxin- and microorganism concentration-dependent; (v) the process did not involve chemical modification of FB1 molecules; and (vi) cell wall structural integrity of the microorganisms was required for FB1 removal. Consequently, we propose that the mechanism involved in the removal of FB1 is a physical adsorption (physisorption) of the toxin molecule to cell wall components of the microorganisms. It is highly probable that FB1 and AFB1 co-occur in contaminated foods, since the fungal genera Aspergillus and Fusarium frequently occur simultaneously. Therefore, we analyzed whether the presence of AFB1 interferes with the removal of FB1 by the microorganisms previously evaluated, and vice versa. Studies of co-occurrence of both mycotoxins clearly showed that they did not compete for binding sites on the microorganism cell wall and the presence of one toxin did not modify the efficiency of the organism in the removal of the other mycotoxin. These findings may be useful for optimization of mycotoxin binding and provide an important contribution to research on microorganisms with ability to remove these secondary metabolites.
Fil: Pizzolitto, Romina Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Salvano, Mario Armando. Universidad Nacional de Río Cuarto; Argentina
Fil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
Materia
AFLATOXIN B 1
FUMONISIN B 1
LACTIC ACID BACTERIA
PHYSICAL ADSORPTION
SACCHAROMYCES CEREVISIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196128
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/196128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding processPizzolitto, Romina PaolaSalvano, Mario ArmandoDalcero, Ana MariaAFLATOXIN B 1FUMONISIN B 1LACTIC ACID BACTERIAPHYSICAL ADSORPTIONSACCHAROMYCES CEREVISIAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objectives of this investigation were to evaluate the ability of Saccharomyces cerevisiae CECT 1891 and Lactobacillus acidophilus 24 to remove fumonisin B1 (FB1) from liquid medium; to determine the nature of the mechanism involved in FB1?microorganism interaction and to analyze whether the presence of aflatoxin B1 (AFB1) interferes with the removal of FB1 and vice versa. The results obtained indicated that: (i) both microorganisms were able to remove FB1 from liquid medium; (ii) the removal was a fast and reversible process; (iii) cell viability was not necessary; (iv) the amount of FB1 removed was both toxin- and microorganism concentration-dependent; (v) the process did not involve chemical modification of FB1 molecules; and (vi) cell wall structural integrity of the microorganisms was required for FB1 removal. Consequently, we propose that the mechanism involved in the removal of FB1 is a physical adsorption (physisorption) of the toxin molecule to cell wall components of the microorganisms. It is highly probable that FB1 and AFB1 co-occur in contaminated foods, since the fungal genera Aspergillus and Fusarium frequently occur simultaneously. Therefore, we analyzed whether the presence of AFB1 interferes with the removal of FB1 by the microorganisms previously evaluated, and vice versa. Studies of co-occurrence of both mycotoxins clearly showed that they did not compete for binding sites on the microorganism cell wall and the presence of one toxin did not modify the efficiency of the organism in the removal of the other mycotoxin. These findings may be useful for optimization of mycotoxin binding and provide an important contribution to research on microorganisms with ability to remove these secondary metabolites.Fil: Pizzolitto, Romina Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Salvano, Mario Armando. Universidad Nacional de Río Cuarto; ArgentinaFil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaElsevier Science2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196128Pizzolitto, Romina Paola; Salvano, Mario Armando; Dalcero, Ana Maria; Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process; Elsevier Science; International Journal of Food Microbiology; 156; 3; 5-2012; 214-2210168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168160512001560info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2012.03.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:33Zoai:ri.conicet.gov.ar:11336/196128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:33.874CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
title Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
spellingShingle Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
Pizzolitto, Romina Paola
AFLATOXIN B 1
FUMONISIN B 1
LACTIC ACID BACTERIA
PHYSICAL ADSORPTION
SACCHAROMYCES CEREVISIAE
title_short Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
title_full Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
title_fullStr Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
title_full_unstemmed Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
title_sort Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process
dc.creator.none.fl_str_mv Pizzolitto, Romina Paola
Salvano, Mario Armando
Dalcero, Ana Maria
author Pizzolitto, Romina Paola
author_facet Pizzolitto, Romina Paola
Salvano, Mario Armando
Dalcero, Ana Maria
author_role author
author2 Salvano, Mario Armando
Dalcero, Ana Maria
author2_role author
author
dc.subject.none.fl_str_mv AFLATOXIN B 1
FUMONISIN B 1
LACTIC ACID BACTERIA
PHYSICAL ADSORPTION
SACCHAROMYCES CEREVISIAE
topic AFLATOXIN B 1
FUMONISIN B 1
LACTIC ACID BACTERIA
PHYSICAL ADSORPTION
SACCHAROMYCES CEREVISIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objectives of this investigation were to evaluate the ability of Saccharomyces cerevisiae CECT 1891 and Lactobacillus acidophilus 24 to remove fumonisin B1 (FB1) from liquid medium; to determine the nature of the mechanism involved in FB1?microorganism interaction and to analyze whether the presence of aflatoxin B1 (AFB1) interferes with the removal of FB1 and vice versa. The results obtained indicated that: (i) both microorganisms were able to remove FB1 from liquid medium; (ii) the removal was a fast and reversible process; (iii) cell viability was not necessary; (iv) the amount of FB1 removed was both toxin- and microorganism concentration-dependent; (v) the process did not involve chemical modification of FB1 molecules; and (vi) cell wall structural integrity of the microorganisms was required for FB1 removal. Consequently, we propose that the mechanism involved in the removal of FB1 is a physical adsorption (physisorption) of the toxin molecule to cell wall components of the microorganisms. It is highly probable that FB1 and AFB1 co-occur in contaminated foods, since the fungal genera Aspergillus and Fusarium frequently occur simultaneously. Therefore, we analyzed whether the presence of AFB1 interferes with the removal of FB1 by the microorganisms previously evaluated, and vice versa. Studies of co-occurrence of both mycotoxins clearly showed that they did not compete for binding sites on the microorganism cell wall and the presence of one toxin did not modify the efficiency of the organism in the removal of the other mycotoxin. These findings may be useful for optimization of mycotoxin binding and provide an important contribution to research on microorganisms with ability to remove these secondary metabolites.
Fil: Pizzolitto, Romina Paola. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Salvano, Mario Armando. Universidad Nacional de Río Cuarto; Argentina
Fil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; Argentina
description The objectives of this investigation were to evaluate the ability of Saccharomyces cerevisiae CECT 1891 and Lactobacillus acidophilus 24 to remove fumonisin B1 (FB1) from liquid medium; to determine the nature of the mechanism involved in FB1?microorganism interaction and to analyze whether the presence of aflatoxin B1 (AFB1) interferes with the removal of FB1 and vice versa. The results obtained indicated that: (i) both microorganisms were able to remove FB1 from liquid medium; (ii) the removal was a fast and reversible process; (iii) cell viability was not necessary; (iv) the amount of FB1 removed was both toxin- and microorganism concentration-dependent; (v) the process did not involve chemical modification of FB1 molecules; and (vi) cell wall structural integrity of the microorganisms was required for FB1 removal. Consequently, we propose that the mechanism involved in the removal of FB1 is a physical adsorption (physisorption) of the toxin molecule to cell wall components of the microorganisms. It is highly probable that FB1 and AFB1 co-occur in contaminated foods, since the fungal genera Aspergillus and Fusarium frequently occur simultaneously. Therefore, we analyzed whether the presence of AFB1 interferes with the removal of FB1 by the microorganisms previously evaluated, and vice versa. Studies of co-occurrence of both mycotoxins clearly showed that they did not compete for binding sites on the microorganism cell wall and the presence of one toxin did not modify the efficiency of the organism in the removal of the other mycotoxin. These findings may be useful for optimization of mycotoxin binding and provide an important contribution to research on microorganisms with ability to remove these secondary metabolites.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196128
Pizzolitto, Romina Paola; Salvano, Mario Armando; Dalcero, Ana Maria; Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process; Elsevier Science; International Journal of Food Microbiology; 156; 3; 5-2012; 214-221
0168-1605
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196128
identifier_str_mv Pizzolitto, Romina Paola; Salvano, Mario Armando; Dalcero, Ana Maria; Analysis of fumonisin B 1 removal by microorganisms in co-occurrence with aflatoxin B 1 and the nature of the binding process; Elsevier Science; International Journal of Food Microbiology; 156; 3; 5-2012; 214-221
0168-1605
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0168160512001560
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2012.03.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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