Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae

Autores
Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Araque, Gustavo Adolfo; Gómez, Juliana Andrea; Guevara, Elsa Judith; Cerrudo, Carolina Susana; Belaich, Mariano Nicolas
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spodoptera ornithogalli (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting S. ornithogalli were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in Baculoviridae, named as Spodoptera ornithogalli nucleopolyhedrovirus (SporNPV) and Spodoptera ornithogalli granulovirus (SporGV). The bioassays carried out in larvae of S. ornithogalli and S. frugiperda showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype.
Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Villamizar, Laura Fernanda. Lincoln Research Centre; Estados Unidos
Fil: Araque, Gustavo Adolfo. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Gómez, Juliana Andrea. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Guevara, Elsa Judith. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; Argentina
Fil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; Argentina
Materia
NATURAL COINFECTION
SPODOPTERA FRUGIPERDA
SPODOPTERA ORNITHOGALLI
SPORGV
SPORNPV
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/166758
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/166758
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvaeBarrera, Gloria PatriciaVillamizar, Laura FernandaAraque, Gustavo AdolfoGómez, Juliana AndreaGuevara, Elsa JudithCerrudo, Carolina SusanaBelaich, Mariano NicolasNATURAL COINFECTIONSPODOPTERA FRUGIPERDASPODOPTERA ORNITHOGALLISPORGVSPORNPVhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Spodoptera ornithogalli (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting S. ornithogalli were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in Baculoviridae, named as Spodoptera ornithogalli nucleopolyhedrovirus (SporNPV) and Spodoptera ornithogalli granulovirus (SporGV). The bioassays carried out in larvae of S. ornithogalli and S. frugiperda showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype.Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Villamizar, Laura Fernanda. Lincoln Research Centre; Estados UnidosFil: Araque, Gustavo Adolfo. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Gómez, Juliana Andrea. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Guevara, Elsa Judith. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; ArgentinaFil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; ArgentinaMultidisciplinary Digital Publishing Institute2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/166758Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Araque, Gustavo Adolfo; Gómez, Juliana Andrea; Guevara, Elsa Judith; et al.; Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae; Multidisciplinary Digital Publishing Institute; Viruses; 13; 12; 12-2021; 1-151999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/13/12/2520info:eu-repo/semantics/altIdentifier/doi/10.3390/v13122520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:47Zoai:ri.conicet.gov.ar:11336/166758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:47.292CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
title Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
spellingShingle Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
Barrera, Gloria Patricia
NATURAL COINFECTION
SPODOPTERA FRUGIPERDA
SPODOPTERA ORNITHOGALLI
SPORGV
SPORNPV
title_short Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
title_full Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
title_fullStr Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
title_full_unstemmed Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
title_sort Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae
dc.creator.none.fl_str_mv Barrera, Gloria Patricia
Villamizar, Laura Fernanda
Araque, Gustavo Adolfo
Gómez, Juliana Andrea
Guevara, Elsa Judith
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author Barrera, Gloria Patricia
author_facet Barrera, Gloria Patricia
Villamizar, Laura Fernanda
Araque, Gustavo Adolfo
Gómez, Juliana Andrea
Guevara, Elsa Judith
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author_role author
author2 Villamizar, Laura Fernanda
Araque, Gustavo Adolfo
Gómez, Juliana Andrea
Guevara, Elsa Judith
Cerrudo, Carolina Susana
Belaich, Mariano Nicolas
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv NATURAL COINFECTION
SPODOPTERA FRUGIPERDA
SPODOPTERA ORNITHOGALLI
SPORGV
SPORNPV
topic NATURAL COINFECTION
SPODOPTERA FRUGIPERDA
SPODOPTERA ORNITHOGALLI
SPORGV
SPORNPV
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spodoptera ornithogalli (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting S. ornithogalli were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in Baculoviridae, named as Spodoptera ornithogalli nucleopolyhedrovirus (SporNPV) and Spodoptera ornithogalli granulovirus (SporGV). The bioassays carried out in larvae of S. ornithogalli and S. frugiperda showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype.
Fil: Barrera, Gloria Patricia. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Villamizar, Laura Fernanda. Lincoln Research Centre; Estados Unidos
Fil: Araque, Gustavo Adolfo. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Gómez, Juliana Andrea. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Guevara, Elsa Judith. Centro de Investigación Tibaitatá. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Cerrudo, Carolina Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; Argentina
Fil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; Argentina
description Spodoptera ornithogalli (Guenée) (Lepidoptera: Noctuidae) is an important pest in different crops of economic relevance in America. For its control, strategies that include chemicals are usually used; so, the description of entomopathogens would be very useful for the formulation of biopesticides. In this regard, two different baculoviruses affecting S. ornithogalli were isolated in Colombia, with one of them being an NPV and the other a GV. Ultrastructural, molecular, and biological characterization showed that both isolates possess the 38 core genes and are novel species in Baculoviridae, named as Spodoptera ornithogalli nucleopolyhedrovirus (SporNPV) and Spodoptera ornithogalli granulovirus (SporGV). The bioassays carried out in larvae of S. ornithogalli and S. frugiperda showed infectivity in both hosts but being higher in the first. In addition, it was observed that SporGV potentiates the insecticidal action of SporNPV (maximum value in ratio 2.5:97.5). Both viruses are individually infective but coexist in nature, producing mixed infections with a synergistic effect that improves the performance of the NPV and enables the transmission of the GV, which presents a slowly killing phenotype.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/166758
Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Araque, Gustavo Adolfo; Gómez, Juliana Andrea; Guevara, Elsa Judith; et al.; Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae; Multidisciplinary Digital Publishing Institute; Viruses; 13; 12; 12-2021; 1-15
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/166758
identifier_str_mv Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Araque, Gustavo Adolfo; Gómez, Juliana Andrea; Guevara, Elsa Judith; et al.; Natural coinfection between novel species of baculoviruses in spodoptera ornithogalli larvae; Multidisciplinary Digital Publishing Institute; Viruses; 13; 12; 12-2021; 1-15
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/13/12/2520
info:eu-repo/semantics/altIdentifier/doi/10.3390/v13122520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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