Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress

Autores
Ulrich, Maria Noelia; Muñiz Padilla, Esteban Tobias; Corach, Alejandra; Hopp, Horacio Esteban; Tosto, Daniela Sandra
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. Quantitative PCR is the technique of choice for studying gene expression related to herbicide resistance because of its high sensitivity and specificity, although its quantitative accuracy is highly dependent on the stability of the reference genes. Thus, in this study we evaluated the stability of different reference genes of glyphosate-resistant S. halepense. Nine genes frequently used as reference genes were selected: MDH, ADP, PP2A, EIF4α, ACT, ARI8, DnaJ, Hsp70, and ALS1, and their expression analyzed in susceptible and resistant biotypes at 0, 24 and 72 h post-application of glyphosate. The stability was analyzed with the geNorm, NormFinder, and BestKeeper software programs and using the ∆Ct method. RefFinder was used to generate a comprehensive stability ranking. The results showed that PP2A and ARI8 were the most stable genes under the test conditions. EPSPS expression was also verified against the best two and the worst two reference genes. This study provides useful information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Fil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Muñiz Padilla, Esteban Tobias. Universidad Nacional de Entre Ríos; Argentina
Fil: Corach, Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
GENE EXPRESSION
HERBICIDE RESISTANCE
RT-QPCR
WEEDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182276
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stressUlrich, Maria NoeliaMuñiz Padilla, Esteban TobiasCorach, AlejandraHopp, Horacio EstebanTosto, Daniela SandraGENE EXPRESSIONHERBICIDE RESISTANCERT-QPCRWEEDShttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. Quantitative PCR is the technique of choice for studying gene expression related to herbicide resistance because of its high sensitivity and specificity, although its quantitative accuracy is highly dependent on the stability of the reference genes. Thus, in this study we evaluated the stability of different reference genes of glyphosate-resistant S. halepense. Nine genes frequently used as reference genes were selected: MDH, ADP, PP2A, EIF4α, ACT, ARI8, DnaJ, Hsp70, and ALS1, and their expression analyzed in susceptible and resistant biotypes at 0, 24 and 72 h post-application of glyphosate. The stability was analyzed with the geNorm, NormFinder, and BestKeeper software programs and using the ∆Ct method. RefFinder was used to generate a comprehensive stability ranking. The results showed that PP2A and ARI8 were the most stable genes under the test conditions. EPSPS expression was also verified against the best two and the worst two reference genes. This study provides useful information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.Fil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Muñiz Padilla, Esteban Tobias. Universidad Nacional de Entre Ríos; ArgentinaFil: Corach, Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMultidisciplinary Digital Publishing Institute2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182276Ulrich, Maria Noelia; Muñiz Padilla, Esteban Tobias; Corach, Alejandra; Hopp, Horacio Esteban; Tosto, Daniela Sandra; Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress; Multidisciplinary Digital Publishing Institute; Plants; 10; 8; 8-2021; 1-132223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/plants10081555info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/8/1555info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:41Zoai:ri.conicet.gov.ar:11336/182276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:41.708CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
title Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
spellingShingle Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
Ulrich, Maria Noelia
GENE EXPRESSION
HERBICIDE RESISTANCE
RT-QPCR
WEEDS
title_short Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
title_full Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
title_fullStr Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
title_full_unstemmed Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
title_sort Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress
dc.creator.none.fl_str_mv Ulrich, Maria Noelia
Muñiz Padilla, Esteban Tobias
Corach, Alejandra
Hopp, Horacio Esteban
Tosto, Daniela Sandra
author Ulrich, Maria Noelia
author_facet Ulrich, Maria Noelia
Muñiz Padilla, Esteban Tobias
Corach, Alejandra
Hopp, Horacio Esteban
Tosto, Daniela Sandra
author_role author
author2 Muñiz Padilla, Esteban Tobias
Corach, Alejandra
Hopp, Horacio Esteban
Tosto, Daniela Sandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv GENE EXPRESSION
HERBICIDE RESISTANCE
RT-QPCR
WEEDS
topic GENE EXPRESSION
HERBICIDE RESISTANCE
RT-QPCR
WEEDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. Quantitative PCR is the technique of choice for studying gene expression related to herbicide resistance because of its high sensitivity and specificity, although its quantitative accuracy is highly dependent on the stability of the reference genes. Thus, in this study we evaluated the stability of different reference genes of glyphosate-resistant S. halepense. Nine genes frequently used as reference genes were selected: MDH, ADP, PP2A, EIF4α, ACT, ARI8, DnaJ, Hsp70, and ALS1, and their expression analyzed in susceptible and resistant biotypes at 0, 24 and 72 h post-application of glyphosate. The stability was analyzed with the geNorm, NormFinder, and BestKeeper software programs and using the ∆Ct method. RefFinder was used to generate a comprehensive stability ranking. The results showed that PP2A and ARI8 were the most stable genes under the test conditions. EPSPS expression was also verified against the best two and the worst two reference genes. This study provides useful information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Fil: Ulrich, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Muñiz Padilla, Esteban Tobias. Universidad Nacional de Entre Ríos; Argentina
Fil: Corach, Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. Quantitative PCR is the technique of choice for studying gene expression related to herbicide resistance because of its high sensitivity and specificity, although its quantitative accuracy is highly dependent on the stability of the reference genes. Thus, in this study we evaluated the stability of different reference genes of glyphosate-resistant S. halepense. Nine genes frequently used as reference genes were selected: MDH, ADP, PP2A, EIF4α, ACT, ARI8, DnaJ, Hsp70, and ALS1, and their expression analyzed in susceptible and resistant biotypes at 0, 24 and 72 h post-application of glyphosate. The stability was analyzed with the geNorm, NormFinder, and BestKeeper software programs and using the ∆Ct method. RefFinder was used to generate a comprehensive stability ranking. The results showed that PP2A and ARI8 were the most stable genes under the test conditions. EPSPS expression was also verified against the best two and the worst two reference genes. This study provides useful information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182276
Ulrich, Maria Noelia; Muñiz Padilla, Esteban Tobias; Corach, Alejandra; Hopp, Horacio Esteban; Tosto, Daniela Sandra; Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress; Multidisciplinary Digital Publishing Institute; Plants; 10; 8; 8-2021; 1-13
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182276
identifier_str_mv Ulrich, Maria Noelia; Muñiz Padilla, Esteban Tobias; Corach, Alejandra; Hopp, Horacio Esteban; Tosto, Daniela Sandra; Validation of reference genes for quantitative pcr in johnsongrass (Sorghum halepense l.) under glyphosate stress; Multidisciplinary Digital Publishing Institute; Plants; 10; 8; 8-2021; 1-13
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10081555
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/8/1555
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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