Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport

Autores
del Balzo, Diego Damián; Capmany, Anahi; Cebrián, José Ignacio; Damiani, Maria Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterium that replicates inside a vesicle called inclusion. Ct manipulatesRab GTPases, master controllers of vesicular transport, to ensure its survival and replication. Dendritic cells (DC) are the most powerful antigenpresenting cells and an essential link between innate and adaptive immunity. We have shown that Ct intercepts Rab14-vesicles to acquire hostlipids necessary for its growth and multiplication. In addition, DC requires Insulin-regulated aminopeptidase (IRAP)-Rab14 endosomes forefficient antigen cross presentation. We hypothesised that Ct recruits Rab14 not only as a strategy for nourishment, but also to interfere withantigen presentation. By confocal microscopy, we observed that Rab14 is associated with the plasma membrane at the entry site of the bacterium.Later, Rab14 is recruited to the chlamydial inclusion membrane and remains there throughout the entire bacterial life cycle. Interestingly, wedistinguished two populations of chlamydial inclusions in DC. On one hand, we found small Ct-containing vesicles that colocalize with EEA1 orLAMP1; and on the other hand, we observed larger ones that recruit Rab14 and exclude markers from the endocytic/degradative pathway.Chlamydial infection did not modify MHC-I expression, shown by western blot. However, infection as well as Rab14 silencing caused aredistribution of MHC-I molecules and interfered with their transport towards the plasma membrane, assessed by flow cytometry. Our resultssuggest that Rab14 is involved in the decrease of antigen cross presentation observed in Ct-infected cells.
Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Fondo para la Investigacion Científica y Tecnológica
Consejo Nacional de Investigaciones Cientificas y Tecnológicas
Agencia Nacional de Promoción Científicas y Tecnológicas
Materia
CHLAMYDIA TRACHOMATIS
ANTIGEN PRESENTATION
RAB GTPASES
INMUNE ESCAPE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275820
Acceder
id CONICETDig_55822cd118d772449cf5634a5ccfe548
oai_identifier_str oai:ri.conicet.gov.ar:11336/275820
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transportdel Balzo, Diego DamiánCapmany, AnahiCebrián, José IgnacioDamiani, Maria ElenaCHLAMYDIA TRACHOMATISANTIGEN PRESENTATIONRAB GTPASESINMUNE ESCAPEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterium that replicates inside a vesicle called inclusion. Ct manipulatesRab GTPases, master controllers of vesicular transport, to ensure its survival and replication. Dendritic cells (DC) are the most powerful antigenpresenting cells and an essential link between innate and adaptive immunity. We have shown that Ct intercepts Rab14-vesicles to acquire hostlipids necessary for its growth and multiplication. In addition, DC requires Insulin-regulated aminopeptidase (IRAP)-Rab14 endosomes forefficient antigen cross presentation. We hypothesised that Ct recruits Rab14 not only as a strategy for nourishment, but also to interfere withantigen presentation. By confocal microscopy, we observed that Rab14 is associated with the plasma membrane at the entry site of the bacterium.Later, Rab14 is recruited to the chlamydial inclusion membrane and remains there throughout the entire bacterial life cycle. Interestingly, wedistinguished two populations of chlamydial inclusions in DC. On one hand, we found small Ct-containing vesicles that colocalize with EEA1 orLAMP1; and on the other hand, we observed larger ones that recruit Rab14 and exclude markers from the endocytic/degradative pathway.Chlamydial infection did not modify MHC-I expression, shown by western blot. However, infection as well as Rab14 silencing caused aredistribution of MHC-I molecules and interfered with their transport towards the plasma membrane, assessed by flow cytometry. Our resultssuggest that Rab14 is involved in the decrease of antigen cross presentation observed in Ct-infected cells.Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaLIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularParanáArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularFondo para la Investigacion Científica y TecnológicaConsejo Nacional de Investigaciones Cientificas y TecnológicasAgencia Nacional de Promoción Científicas y TecnológicasTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275820Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport; LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 84-841667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-42.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:57:03Zoai:ri.conicet.gov.ar:11336/275820instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:57:03.38CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
title Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
spellingShingle Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
del Balzo, Diego Damián
CHLAMYDIA TRACHOMATIS
ANTIGEN PRESENTATION
RAB GTPASES
INMUNE ESCAPE
title_short Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
title_full Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
title_fullStr Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
title_full_unstemmed Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
title_sort Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport
dc.creator.none.fl_str_mv del Balzo, Diego Damián
Capmany, Anahi
Cebrián, José Ignacio
Damiani, Maria Elena
author del Balzo, Diego Damián
author_facet del Balzo, Diego Damián
Capmany, Anahi
Cebrián, José Ignacio
Damiani, Maria Elena
author_role author
author2 Capmany, Anahi
Cebrián, José Ignacio
Damiani, Maria Elena
author2_role author
author
author
dc.subject.none.fl_str_mv CHLAMYDIA TRACHOMATIS
ANTIGEN PRESENTATION
RAB GTPASES
INMUNE ESCAPE
topic CHLAMYDIA TRACHOMATIS
ANTIGEN PRESENTATION
RAB GTPASES
INMUNE ESCAPE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterium that replicates inside a vesicle called inclusion. Ct manipulatesRab GTPases, master controllers of vesicular transport, to ensure its survival and replication. Dendritic cells (DC) are the most powerful antigenpresenting cells and an essential link between innate and adaptive immunity. We have shown that Ct intercepts Rab14-vesicles to acquire hostlipids necessary for its growth and multiplication. In addition, DC requires Insulin-regulated aminopeptidase (IRAP)-Rab14 endosomes forefficient antigen cross presentation. We hypothesised that Ct recruits Rab14 not only as a strategy for nourishment, but also to interfere withantigen presentation. By confocal microscopy, we observed that Rab14 is associated with the plasma membrane at the entry site of the bacterium.Later, Rab14 is recruited to the chlamydial inclusion membrane and remains there throughout the entire bacterial life cycle. Interestingly, wedistinguished two populations of chlamydial inclusions in DC. On one hand, we found small Ct-containing vesicles that colocalize with EEA1 orLAMP1; and on the other hand, we observed larger ones that recruit Rab14 and exclude markers from the endocytic/degradative pathway.Chlamydial infection did not modify MHC-I expression, shown by western blot. However, infection as well as Rab14 silencing caused aredistribution of MHC-I molecules and interfered with their transport towards the plasma membrane, assessed by flow cytometry. Our resultssuggest that Rab14 is involved in the decrease of antigen cross presentation observed in Ct-infected cells.
Fil: del Balzo, Diego Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Fondo para la Investigacion Científica y Tecnológica
Consejo Nacional de Investigaciones Cientificas y Tecnológicas
Agencia Nacional de Promoción Científicas y Tecnológicas
description Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterium that replicates inside a vesicle called inclusion. Ct manipulatesRab GTPases, master controllers of vesicular transport, to ensure its survival and replication. Dendritic cells (DC) are the most powerful antigenpresenting cells and an essential link between innate and adaptive immunity. We have shown that Ct intercepts Rab14-vesicles to acquire hostlipids necessary for its growth and multiplication. In addition, DC requires Insulin-regulated aminopeptidase (IRAP)-Rab14 endosomes forefficient antigen cross presentation. We hypothesised that Ct recruits Rab14 not only as a strategy for nourishment, but also to interfere withantigen presentation. By confocal microscopy, we observed that Rab14 is associated with the plasma membrane at the entry site of the bacterium.Later, Rab14 is recruited to the chlamydial inclusion membrane and remains there throughout the entire bacterial life cycle. Interestingly, wedistinguished two populations of chlamydial inclusions in DC. On one hand, we found small Ct-containing vesicles that colocalize with EEA1 orLAMP1; and on the other hand, we observed larger ones that recruit Rab14 and exclude markers from the endocytic/degradative pathway.Chlamydial infection did not modify MHC-I expression, shown by western blot. However, infection as well as Rab14 silencing caused aredistribution of MHC-I molecules and interfered with their transport towards the plasma membrane, assessed by flow cytometry. Our resultssuggest that Rab14 is involved in the decrease of antigen cross presentation observed in Ct-infected cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275820
Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport; LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 84-84
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275820
identifier_str_mv Chlamydia trachomatis perturbs antigen cross presentation by interfering Rab14- controlled transport; LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 84-84
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-42.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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