Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses
- Autores
- Velasquez, Lis Noelia; Stüve, Philipp; Gentilini, Maria Virginia; Swallow, Maxine; Bartel, Judith; Lycke, Nils Yngve; Barkan, Daniel; Martina, Mariana Andrea; Lujan, Hugo Daniel; Kalay, Hakan; van Kooyk, Yvette; Sparwasser, Tim D.; Berod, Luciana
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tuberculosis remains a major global health problem and efforts to develop a more effective vaccine have been unsuccessful so far. Targeting antigens (Ags) to dendritic cells (DCs) in vivo has emerged as a new promising vaccine strategy. In this approach, Ags are delivered directly to DCs via antibodies that bind to endocytic cell-surface receptors. Here, we explored DC-specific-ICAM3-grabbing-nonintegrin (DC-SIGN) targeting as a potential vaccine against tuberculosis. For this, we made use of the hSIGN mouse model that expresses human DC-SIGN under the control of the murine CD11c promoter. We show that in vitro and in vivo delivery of anti-DC-SIGN antibodies conjugated to Ag85B and peptide 25 of Ag85B in combination with anti-CD40, the fungal cell wall component zymosan, and the cholera toxin-derived fusion protein CTA1-DD induces strong Ag-specific CD4+ T-cell responses. Improved anti-mycobacterial immunity was accompanied by increased frequencies of Ag-specific IFN-γ+ IL-2+ TNF-α+ polyfunctional CD4+ T cells in vaccinated mice compared with controls. Taken together, in this study we provide the proof of concept that the human DC-SIGN receptor can be efficiently exploited for vaccine purposes to promote immunity against mycobacterial infections.
Fil: Velasquez, Lis Noelia. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania
Fil: Stüve, Philipp. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania
Fil: Gentilini, Maria Virginia. Medical School Hannover; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania
Fil: Swallow, Maxine. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania
Fil: Bartel, Judith. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania
Fil: Lycke, Nils Yngve. Universidad de Gotemburgo; Suecia
Fil: Barkan, Daniel. The Hebrew University of Jerusalem; Israel
Fil: Martina, Mariana Andrea. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lujan, Hugo Daniel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kalay, Hakan. VU University Medical Center; Países Bajos
Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos
Fil: Sparwasser, Tim D.. Centre for Experimental and Clinical Infection Research; Alemania. Medical School Hannover; Alemania. Helmholtz Centre for Infection Research; Alemania
Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania - Materia
-
AG85B
DC-SPECIFIC-ICAM3-GRABBING-NONINTEGRIN
DENDRITIC CELLS
TUBERCULOSIS
VACCINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/99508
Ver los metadatos del registro completo
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Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responsesVelasquez, Lis NoeliaStüve, PhilippGentilini, Maria VirginiaSwallow, MaxineBartel, JudithLycke, Nils YngveBarkan, DanielMartina, Mariana AndreaLujan, Hugo DanielKalay, Hakanvan Kooyk, YvetteSparwasser, Tim D.Berod, LucianaAG85BDC-SPECIFIC-ICAM3-GRABBING-NONINTEGRINDENDRITIC CELLSTUBERCULOSISVACCINEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Tuberculosis remains a major global health problem and efforts to develop a more effective vaccine have been unsuccessful so far. Targeting antigens (Ags) to dendritic cells (DCs) in vivo has emerged as a new promising vaccine strategy. In this approach, Ags are delivered directly to DCs via antibodies that bind to endocytic cell-surface receptors. Here, we explored DC-specific-ICAM3-grabbing-nonintegrin (DC-SIGN) targeting as a potential vaccine against tuberculosis. For this, we made use of the hSIGN mouse model that expresses human DC-SIGN under the control of the murine CD11c promoter. We show that in vitro and in vivo delivery of anti-DC-SIGN antibodies conjugated to Ag85B and peptide 25 of Ag85B in combination with anti-CD40, the fungal cell wall component zymosan, and the cholera toxin-derived fusion protein CTA1-DD induces strong Ag-specific CD4+ T-cell responses. Improved anti-mycobacterial immunity was accompanied by increased frequencies of Ag-specific IFN-γ+ IL-2+ TNF-α+ polyfunctional CD4+ T cells in vaccinated mice compared with controls. Taken together, in this study we provide the proof of concept that the human DC-SIGN receptor can be efficiently exploited for vaccine purposes to promote immunity against mycobacterial infections.Fil: Velasquez, Lis Noelia. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; AlemaniaFil: Stüve, Philipp. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; AlemaniaFil: Gentilini, Maria Virginia. Medical School Hannover; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; AlemaniaFil: Swallow, Maxine. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; AlemaniaFil: Bartel, Judith. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; AlemaniaFil: Lycke, Nils Yngve. Universidad de Gotemburgo; SueciaFil: Barkan, Daniel. The Hebrew University of Jerusalem; IsraelFil: Martina, Mariana Andrea. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lujan, Hugo Daniel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kalay, Hakan. VU University Medical Center; Países BajosFil: van Kooyk, Yvette. VU University Medical Center; Países BajosFil: Sparwasser, Tim D.. Centre for Experimental and Clinical Infection Research; Alemania. Medical School Hannover; Alemania. Helmholtz Centre for Infection Research; AlemaniaFil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; AlemaniaFrontiers Media S.A.2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99508Velasquez, Lis Noelia; Stüve, Philipp; Gentilini, Maria Virginia; Swallow, Maxine; Bartel, Judith; et al.; Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses; Frontiers Media S.A.; Frontiers in Immunology; 9; MAR; 3-2018; 1-141664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fimmu.2018.00471/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2018.00471info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:27Zoai:ri.conicet.gov.ar:11336/99508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:28.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
title |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
spellingShingle |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses Velasquez, Lis Noelia AG85B DC-SPECIFIC-ICAM3-GRABBING-NONINTEGRIN DENDRITIC CELLS TUBERCULOSIS VACCINE |
title_short |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
title_full |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
title_fullStr |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
title_full_unstemmed |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
title_sort |
Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses |
dc.creator.none.fl_str_mv |
Velasquez, Lis Noelia Stüve, Philipp Gentilini, Maria Virginia Swallow, Maxine Bartel, Judith Lycke, Nils Yngve Barkan, Daniel Martina, Mariana Andrea Lujan, Hugo Daniel Kalay, Hakan van Kooyk, Yvette Sparwasser, Tim D. Berod, Luciana |
author |
Velasquez, Lis Noelia |
author_facet |
Velasquez, Lis Noelia Stüve, Philipp Gentilini, Maria Virginia Swallow, Maxine Bartel, Judith Lycke, Nils Yngve Barkan, Daniel Martina, Mariana Andrea Lujan, Hugo Daniel Kalay, Hakan van Kooyk, Yvette Sparwasser, Tim D. Berod, Luciana |
author_role |
author |
author2 |
Stüve, Philipp Gentilini, Maria Virginia Swallow, Maxine Bartel, Judith Lycke, Nils Yngve Barkan, Daniel Martina, Mariana Andrea Lujan, Hugo Daniel Kalay, Hakan van Kooyk, Yvette Sparwasser, Tim D. Berod, Luciana |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
AG85B DC-SPECIFIC-ICAM3-GRABBING-NONINTEGRIN DENDRITIC CELLS TUBERCULOSIS VACCINE |
topic |
AG85B DC-SPECIFIC-ICAM3-GRABBING-NONINTEGRIN DENDRITIC CELLS TUBERCULOSIS VACCINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Tuberculosis remains a major global health problem and efforts to develop a more effective vaccine have been unsuccessful so far. Targeting antigens (Ags) to dendritic cells (DCs) in vivo has emerged as a new promising vaccine strategy. In this approach, Ags are delivered directly to DCs via antibodies that bind to endocytic cell-surface receptors. Here, we explored DC-specific-ICAM3-grabbing-nonintegrin (DC-SIGN) targeting as a potential vaccine against tuberculosis. For this, we made use of the hSIGN mouse model that expresses human DC-SIGN under the control of the murine CD11c promoter. We show that in vitro and in vivo delivery of anti-DC-SIGN antibodies conjugated to Ag85B and peptide 25 of Ag85B in combination with anti-CD40, the fungal cell wall component zymosan, and the cholera toxin-derived fusion protein CTA1-DD induces strong Ag-specific CD4+ T-cell responses. Improved anti-mycobacterial immunity was accompanied by increased frequencies of Ag-specific IFN-γ+ IL-2+ TNF-α+ polyfunctional CD4+ T cells in vaccinated mice compared with controls. Taken together, in this study we provide the proof of concept that the human DC-SIGN receptor can be efficiently exploited for vaccine purposes to promote immunity against mycobacterial infections. Fil: Velasquez, Lis Noelia. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania Fil: Stüve, Philipp. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania Fil: Gentilini, Maria Virginia. Medical School Hannover; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania Fil: Swallow, Maxine. Medical School Hannover; Alemania. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania Fil: Bartel, Judith. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania Fil: Lycke, Nils Yngve. Universidad de Gotemburgo; Suecia Fil: Barkan, Daniel. The Hebrew University of Jerusalem; Israel Fil: Martina, Mariana Andrea. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lujan, Hugo Daniel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kalay, Hakan. VU University Medical Center; Países Bajos Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos Fil: Sparwasser, Tim D.. Centre for Experimental and Clinical Infection Research; Alemania. Medical School Hannover; Alemania. Helmholtz Centre for Infection Research; Alemania Fil: Berod, Luciana. Centre for Experimental and Clinical Infection Research; Alemania. Helmholtz Centre for Infection Research; Alemania. Medical School Hannover; Alemania |
description |
Tuberculosis remains a major global health problem and efforts to develop a more effective vaccine have been unsuccessful so far. Targeting antigens (Ags) to dendritic cells (DCs) in vivo has emerged as a new promising vaccine strategy. In this approach, Ags are delivered directly to DCs via antibodies that bind to endocytic cell-surface receptors. Here, we explored DC-specific-ICAM3-grabbing-nonintegrin (DC-SIGN) targeting as a potential vaccine against tuberculosis. For this, we made use of the hSIGN mouse model that expresses human DC-SIGN under the control of the murine CD11c promoter. We show that in vitro and in vivo delivery of anti-DC-SIGN antibodies conjugated to Ag85B and peptide 25 of Ag85B in combination with anti-CD40, the fungal cell wall component zymosan, and the cholera toxin-derived fusion protein CTA1-DD induces strong Ag-specific CD4+ T-cell responses. Improved anti-mycobacterial immunity was accompanied by increased frequencies of Ag-specific IFN-γ+ IL-2+ TNF-α+ polyfunctional CD4+ T cells in vaccinated mice compared with controls. Taken together, in this study we provide the proof of concept that the human DC-SIGN receptor can be efficiently exploited for vaccine purposes to promote immunity against mycobacterial infections. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/99508 Velasquez, Lis Noelia; Stüve, Philipp; Gentilini, Maria Virginia; Swallow, Maxine; Bartel, Judith; et al.; Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses; Frontiers Media S.A.; Frontiers in Immunology; 9; MAR; 3-2018; 1-14 1664-3224 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/99508 |
identifier_str_mv |
Velasquez, Lis Noelia; Stüve, Philipp; Gentilini, Maria Virginia; Swallow, Maxine; Bartel, Judith; et al.; Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses; Frontiers Media S.A.; Frontiers in Immunology; 9; MAR; 3-2018; 1-14 1664-3224 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fimmu.2018.00471/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2018.00471 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613555368755200 |
score |
13.070432 |