Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes

Autores
Vazquez, Maria Isabel; Forcada, F.; Sosa, C.; Casao, A.; Sartore, I.; Fernandez Foren, A.; Meikle, A.; Abecia, J. C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of exogenous melatonin on embryo viability in undernourished ewes was investigated. At lambing, 24 ewes were treated (+MEL) or not (−MEL) with a melatonin implant. After 45 days, both groups were fed to provide 1.5 (Control, C) or 0.5 (Low, L) times daily maintenance requirements, so that experimental groups were: C−MEL, C+MEL, L−MEL and L+MEL. Ewes were mated (Day 0) and on Day 5 embryos were recovered and classified according to their developmental stage and morphology. Ovaries were used for in vitro fertilization and uterine horns were processed to study progesterone and oestrogen receptor (PR and ERα) expression by inmunohistochemistry. After 21 days, groups L−MEL and L+MEL had an average weight loss of 10 kg (P < 0.001). Number of viable embryos per CL from L+MEL (0.50 ± 0.2) was higher than from other groups (P < 0.05). Overall, the melatonin effect was particularly evident in undernourished ewes, increasing both viability (L+MEL: 65%; L−MEL: 25%; P < 0.05) and pregnancy rates (L+MEL: 66.6%; L−MEL: 16.6%; P < 0.05). Neither nutrition and melatonin nor their interaction had a significant effect on the in vitro oocyte development. Melatonin treatment tended to increase the percentage of positive cells to PR in deep glandular epithelium, independently of diet (P = 0.09), and the greatest staining intensity of PR was observed in the luminal and superficial glandular epithelia (P < 0.0001). In conclusion, melatonin implants at lambing during the breeding season improve the viability of embryos recovered from undernourished ewes, although this effect seems not to be mediated at the oocyte competence level.
Fil: Vazquez, Maria Isabel. Universidad de Zaragoza; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Forcada, F.. Universidad de Zaragoza; España
Fil: Sosa, C.. Universidad de Zaragoza; España
Fil: Casao, A.. Universidad de Zaragoza; España
Fil: Sartore, I.. Universidad de la República; Uruguay
Fil: Fernandez Foren, A.. Universidad de la República; Uruguay
Fil: Meikle, A.. Universidad de la República; Uruguay
Fil: Abecia, J. C.. Universidad de Zaragoza; España
Materia
Sheep
Embryo
Undernutrition
Melatonin
Oocyte
Endometrial Receptors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23994
Acceder
id CONICETDig_52b975eebccbafb8af5d177e4a588aba
oai_identifier_str oai:ri.conicet.gov.ar:11336/23994
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewesVazquez, Maria IsabelForcada, F.Sosa, C.Casao, A.Sartore, I.Fernandez Foren, A.Meikle, A.Abecia, J. C.SheepEmbryoUndernutritionMelatoninOocyteEndometrial Receptorshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The effect of exogenous melatonin on embryo viability in undernourished ewes was investigated. At lambing, 24 ewes were treated (+MEL) or not (−MEL) with a melatonin implant. After 45 days, both groups were fed to provide 1.5 (Control, C) or 0.5 (Low, L) times daily maintenance requirements, so that experimental groups were: C−MEL, C+MEL, L−MEL and L+MEL. Ewes were mated (Day 0) and on Day 5 embryos were recovered and classified according to their developmental stage and morphology. Ovaries were used for in vitro fertilization and uterine horns were processed to study progesterone and oestrogen receptor (PR and ERα) expression by inmunohistochemistry. After 21 days, groups L−MEL and L+MEL had an average weight loss of 10 kg (P < 0.001). Number of viable embryos per CL from L+MEL (0.50 ± 0.2) was higher than from other groups (P < 0.05). Overall, the melatonin effect was particularly evident in undernourished ewes, increasing both viability (L+MEL: 65%; L−MEL: 25%; P < 0.05) and pregnancy rates (L+MEL: 66.6%; L−MEL: 16.6%; P < 0.05). Neither nutrition and melatonin nor their interaction had a significant effect on the in vitro oocyte development. Melatonin treatment tended to increase the percentage of positive cells to PR in deep glandular epithelium, independently of diet (P = 0.09), and the greatest staining intensity of PR was observed in the luminal and superficial glandular epithelia (P < 0.0001). In conclusion, melatonin implants at lambing during the breeding season improve the viability of embryos recovered from undernourished ewes, although this effect seems not to be mediated at the oocyte competence level.Fil: Vazquez, Maria Isabel. Universidad de Zaragoza; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Forcada, F.. Universidad de Zaragoza; EspañaFil: Sosa, C.. Universidad de Zaragoza; EspañaFil: Casao, A.. Universidad de Zaragoza; EspañaFil: Sartore, I.. Universidad de la República; UruguayFil: Fernandez Foren, A.. Universidad de la República; UruguayFil: Meikle, A.. Universidad de la República; UruguayFil: Abecia, J. C.. Universidad de Zaragoza; EspañaElsevier Science2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23994Vazquez, Maria Isabel; Forcada, F.; Sosa, C.; Casao, A.; Sartore, I.; et al.; Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes; Elsevier Science; Animal Reproduction Science; 141; 1-2; 7-2013; 52-610378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2013.07.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378432013002145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:15Zoai:ri.conicet.gov.ar:11336/23994instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:15.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
title Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
spellingShingle Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
Vazquez, Maria Isabel
Sheep
Embryo
Undernutrition
Melatonin
Oocyte
Endometrial Receptors
title_short Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
title_full Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
title_fullStr Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
title_full_unstemmed Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
title_sort Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes
dc.creator.none.fl_str_mv Vazquez, Maria Isabel
Forcada, F.
Sosa, C.
Casao, A.
Sartore, I.
Fernandez Foren, A.
Meikle, A.
Abecia, J. C.
author Vazquez, Maria Isabel
author_facet Vazquez, Maria Isabel
Forcada, F.
Sosa, C.
Casao, A.
Sartore, I.
Fernandez Foren, A.
Meikle, A.
Abecia, J. C.
author_role author
author2 Forcada, F.
Sosa, C.
Casao, A.
Sartore, I.
Fernandez Foren, A.
Meikle, A.
Abecia, J. C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sheep
Embryo
Undernutrition
Melatonin
Oocyte
Endometrial Receptors
topic Sheep
Embryo
Undernutrition
Melatonin
Oocyte
Endometrial Receptors
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The effect of exogenous melatonin on embryo viability in undernourished ewes was investigated. At lambing, 24 ewes were treated (+MEL) or not (−MEL) with a melatonin implant. After 45 days, both groups were fed to provide 1.5 (Control, C) or 0.5 (Low, L) times daily maintenance requirements, so that experimental groups were: C−MEL, C+MEL, L−MEL and L+MEL. Ewes were mated (Day 0) and on Day 5 embryos were recovered and classified according to their developmental stage and morphology. Ovaries were used for in vitro fertilization and uterine horns were processed to study progesterone and oestrogen receptor (PR and ERα) expression by inmunohistochemistry. After 21 days, groups L−MEL and L+MEL had an average weight loss of 10 kg (P < 0.001). Number of viable embryos per CL from L+MEL (0.50 ± 0.2) was higher than from other groups (P < 0.05). Overall, the melatonin effect was particularly evident in undernourished ewes, increasing both viability (L+MEL: 65%; L−MEL: 25%; P < 0.05) and pregnancy rates (L+MEL: 66.6%; L−MEL: 16.6%; P < 0.05). Neither nutrition and melatonin nor their interaction had a significant effect on the in vitro oocyte development. Melatonin treatment tended to increase the percentage of positive cells to PR in deep glandular epithelium, independently of diet (P = 0.09), and the greatest staining intensity of PR was observed in the luminal and superficial glandular epithelia (P < 0.0001). In conclusion, melatonin implants at lambing during the breeding season improve the viability of embryos recovered from undernourished ewes, although this effect seems not to be mediated at the oocyte competence level.
Fil: Vazquez, Maria Isabel. Universidad de Zaragoza; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Forcada, F.. Universidad de Zaragoza; España
Fil: Sosa, C.. Universidad de Zaragoza; España
Fil: Casao, A.. Universidad de Zaragoza; España
Fil: Sartore, I.. Universidad de la República; Uruguay
Fil: Fernandez Foren, A.. Universidad de la República; Uruguay
Fil: Meikle, A.. Universidad de la República; Uruguay
Fil: Abecia, J. C.. Universidad de Zaragoza; España
description The effect of exogenous melatonin on embryo viability in undernourished ewes was investigated. At lambing, 24 ewes were treated (+MEL) or not (−MEL) with a melatonin implant. After 45 days, both groups were fed to provide 1.5 (Control, C) or 0.5 (Low, L) times daily maintenance requirements, so that experimental groups were: C−MEL, C+MEL, L−MEL and L+MEL. Ewes were mated (Day 0) and on Day 5 embryos were recovered and classified according to their developmental stage and morphology. Ovaries were used for in vitro fertilization and uterine horns were processed to study progesterone and oestrogen receptor (PR and ERα) expression by inmunohistochemistry. After 21 days, groups L−MEL and L+MEL had an average weight loss of 10 kg (P < 0.001). Number of viable embryos per CL from L+MEL (0.50 ± 0.2) was higher than from other groups (P < 0.05). Overall, the melatonin effect was particularly evident in undernourished ewes, increasing both viability (L+MEL: 65%; L−MEL: 25%; P < 0.05) and pregnancy rates (L+MEL: 66.6%; L−MEL: 16.6%; P < 0.05). Neither nutrition and melatonin nor their interaction had a significant effect on the in vitro oocyte development. Melatonin treatment tended to increase the percentage of positive cells to PR in deep glandular epithelium, independently of diet (P = 0.09), and the greatest staining intensity of PR was observed in the luminal and superficial glandular epithelia (P < 0.0001). In conclusion, melatonin implants at lambing during the breeding season improve the viability of embryos recovered from undernourished ewes, although this effect seems not to be mediated at the oocyte competence level.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23994
Vazquez, Maria Isabel; Forcada, F.; Sosa, C.; Casao, A.; Sartore, I.; et al.; Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes; Elsevier Science; Animal Reproduction Science; 141; 1-2; 7-2013; 52-61
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23994
identifier_str_mv Vazquez, Maria Isabel; Forcada, F.; Sosa, C.; Casao, A.; Sartore, I.; et al.; Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes; Elsevier Science; Animal Reproduction Science; 141; 1-2; 7-2013; 52-61
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2013.07.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378432013002145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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