Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues

Autores
Reyes, Dardo Antonio; Ponce, Ruth Araceli; Loto, Flavia del Valle; Baigori, Mario Domingo; Pera, Licia Maria
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Industrial residues such as crude glycerol (CG) and waste cooking oil (WCO) pose important ecological and economic problems. Thus, the synthesis of microbial lipids using biotransformation routes constitute an attractive alternative to employ those residues under the circular economy concept. It is known that oleaginous microorganisms are capable of utilizing both hydrophilic and hydrophobic substrates to accumulate lipids via de novo and ex novo, respectively. Besides, current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In submerged fermentation, fungal morphology can vary from compact pellets to dispersed mycelia. Thus, the main objective of this work was to analyze biotransformations yielding lipids mediated by A. niger MYA 135 from CG (100 g/L) or WCO (25 g/L) in the presence of morphological effectors such as talc microparticles (8 g/L), FeCl3 (1 g/L) and MnCl2 (3.6 mg/L). Shake flask fermentation were conducted at 30°C during 96 h with or without the supplementation of effectors according to a 23 factorial design. Under de novo lipid fermentation, lipid content was not significantly affected by fungal pelletization (p=0.315). The maximum lipid content was 32.38 ± 1.35 % of its defatted dry weight (w/w). However, the fatty acid (FA) profile obtaining from pellets (SFA: 19.57 %, PUFA: 36.43 %, MUFA: 44.00 %) was different to that obtaining form dispersed mycelium (SFA: 24.30 %, PUFA: 30.10 %, MUFA: 45.61 %). In addition, a significant interaction between FeCl3 and MnCl2 was detected (p<0.001). Under ex novo lipid fermentation, lipid content was significantly affected by the form of fungal growth (p=0.010). In this case, pelletization favored lipid accumulation. The maximum lipid content was 87.00 ± 1.41 % of its defatted dry weight (w/w). However, the FA profile obtaining from pellets (SFA: 22.01 %, PUFA: 19.92 %, MUFA: 58.07 %) was almost similar to that obtaining from dispersed mycelium (SFA: 21.19 %, PUFA: 22.82 %, MUFA: 55.99 %) and different to that observed from WCO (SFA: 12.11 %, PUFA: 48.32 %, MUFA: 38.31 %). In addition, the interaction between FeCl3 and MnCl2 was not significant (p=0.092). Thus, when the biotransformations swift from de novo to ex novo the FA profiles were changed toward synthesizing higher amounts of MUFA and lower amounts of PUFA.
Fil: Reyes, Dardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ponce, Ruth Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Rosario
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
BIOTRANSFORMATION
INDUSTRIAL RESIDUES
MICROBIAL LIPID
SUBMERGED FERMENTATION
FUNGAL MORPHOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238632
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/238632
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residuesReyes, Dardo AntonioPonce, Ruth AraceliLoto, Flavia del ValleBaigori, Mario DomingoPera, Licia MariaBIOTRANSFORMATIONINDUSTRIAL RESIDUESMICROBIAL LIPIDSUBMERGED FERMENTATIONFUNGAL MORPHOLOGYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Industrial residues such as crude glycerol (CG) and waste cooking oil (WCO) pose important ecological and economic problems. Thus, the synthesis of microbial lipids using biotransformation routes constitute an attractive alternative to employ those residues under the circular economy concept. It is known that oleaginous microorganisms are capable of utilizing both hydrophilic and hydrophobic substrates to accumulate lipids via de novo and ex novo, respectively. Besides, current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In submerged fermentation, fungal morphology can vary from compact pellets to dispersed mycelia. Thus, the main objective of this work was to analyze biotransformations yielding lipids mediated by A. niger MYA 135 from CG (100 g/L) or WCO (25 g/L) in the presence of morphological effectors such as talc microparticles (8 g/L), FeCl3 (1 g/L) and MnCl2 (3.6 mg/L). Shake flask fermentation were conducted at 30°C during 96 h with or without the supplementation of effectors according to a 23 factorial design. Under de novo lipid fermentation, lipid content was not significantly affected by fungal pelletization (p=0.315). The maximum lipid content was 32.38 ± 1.35 % of its defatted dry weight (w/w). However, the fatty acid (FA) profile obtaining from pellets (SFA: 19.57 %, PUFA: 36.43 %, MUFA: 44.00 %) was different to that obtaining form dispersed mycelium (SFA: 24.30 %, PUFA: 30.10 %, MUFA: 45.61 %). In addition, a significant interaction between FeCl3 and MnCl2 was detected (p<0.001). Under ex novo lipid fermentation, lipid content was significantly affected by the form of fungal growth (p=0.010). In this case, pelletization favored lipid accumulation. The maximum lipid content was 87.00 ± 1.41 % of its defatted dry weight (w/w). However, the FA profile obtaining from pellets (SFA: 22.01 %, PUFA: 19.92 %, MUFA: 58.07 %) was almost similar to that obtaining from dispersed mycelium (SFA: 21.19 %, PUFA: 22.82 %, MUFA: 55.99 %) and different to that observed from WCO (SFA: 12.11 %, PUFA: 48.32 %, MUFA: 38.31 %). In addition, the interaction between FeCl3 and MnCl2 was not significant (p=0.092). Thus, when the biotransformations swift from de novo to ex novo the FA profiles were changed toward synthesizing higher amounts of MUFA and lower amounts of PUFA.Fil: Reyes, Dardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ponce, Ruth Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaLIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchRosarioArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238632Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues; LIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Rosario; Argentina; 2023; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2023/abstracts-EN.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:05Zoai:ri.conicet.gov.ar:11336/238632instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:05.715CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
title Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
spellingShingle Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
Reyes, Dardo Antonio
BIOTRANSFORMATION
INDUSTRIAL RESIDUES
MICROBIAL LIPID
SUBMERGED FERMENTATION
FUNGAL MORPHOLOGY
title_short Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
title_full Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
title_fullStr Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
title_full_unstemmed Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
title_sort Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues
dc.creator.none.fl_str_mv Reyes, Dardo Antonio
Ponce, Ruth Araceli
Loto, Flavia del Valle
Baigori, Mario Domingo
Pera, Licia Maria
author Reyes, Dardo Antonio
author_facet Reyes, Dardo Antonio
Ponce, Ruth Araceli
Loto, Flavia del Valle
Baigori, Mario Domingo
Pera, Licia Maria
author_role author
author2 Ponce, Ruth Araceli
Loto, Flavia del Valle
Baigori, Mario Domingo
Pera, Licia Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOTRANSFORMATION
INDUSTRIAL RESIDUES
MICROBIAL LIPID
SUBMERGED FERMENTATION
FUNGAL MORPHOLOGY
topic BIOTRANSFORMATION
INDUSTRIAL RESIDUES
MICROBIAL LIPID
SUBMERGED FERMENTATION
FUNGAL MORPHOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Industrial residues such as crude glycerol (CG) and waste cooking oil (WCO) pose important ecological and economic problems. Thus, the synthesis of microbial lipids using biotransformation routes constitute an attractive alternative to employ those residues under the circular economy concept. It is known that oleaginous microorganisms are capable of utilizing both hydrophilic and hydrophobic substrates to accumulate lipids via de novo and ex novo, respectively. Besides, current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In submerged fermentation, fungal morphology can vary from compact pellets to dispersed mycelia. Thus, the main objective of this work was to analyze biotransformations yielding lipids mediated by A. niger MYA 135 from CG (100 g/L) or WCO (25 g/L) in the presence of morphological effectors such as talc microparticles (8 g/L), FeCl3 (1 g/L) and MnCl2 (3.6 mg/L). Shake flask fermentation were conducted at 30°C during 96 h with or without the supplementation of effectors according to a 23 factorial design. Under de novo lipid fermentation, lipid content was not significantly affected by fungal pelletization (p=0.315). The maximum lipid content was 32.38 ± 1.35 % of its defatted dry weight (w/w). However, the fatty acid (FA) profile obtaining from pellets (SFA: 19.57 %, PUFA: 36.43 %, MUFA: 44.00 %) was different to that obtaining form dispersed mycelium (SFA: 24.30 %, PUFA: 30.10 %, MUFA: 45.61 %). In addition, a significant interaction between FeCl3 and MnCl2 was detected (p<0.001). Under ex novo lipid fermentation, lipid content was significantly affected by the form of fungal growth (p=0.010). In this case, pelletization favored lipid accumulation. The maximum lipid content was 87.00 ± 1.41 % of its defatted dry weight (w/w). However, the FA profile obtaining from pellets (SFA: 22.01 %, PUFA: 19.92 %, MUFA: 58.07 %) was almost similar to that obtaining from dispersed mycelium (SFA: 21.19 %, PUFA: 22.82 %, MUFA: 55.99 %) and different to that observed from WCO (SFA: 12.11 %, PUFA: 48.32 %, MUFA: 38.31 %). In addition, the interaction between FeCl3 and MnCl2 was not significant (p=0.092). Thus, when the biotransformations swift from de novo to ex novo the FA profiles were changed toward synthesizing higher amounts of MUFA and lower amounts of PUFA.
Fil: Reyes, Dardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ponce, Ruth Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Rosario
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Industrial residues such as crude glycerol (CG) and waste cooking oil (WCO) pose important ecological and economic problems. Thus, the synthesis of microbial lipids using biotransformation routes constitute an attractive alternative to employ those residues under the circular economy concept. It is known that oleaginous microorganisms are capable of utilizing both hydrophilic and hydrophobic substrates to accumulate lipids via de novo and ex novo, respectively. Besides, current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In submerged fermentation, fungal morphology can vary from compact pellets to dispersed mycelia. Thus, the main objective of this work was to analyze biotransformations yielding lipids mediated by A. niger MYA 135 from CG (100 g/L) or WCO (25 g/L) in the presence of morphological effectors such as talc microparticles (8 g/L), FeCl3 (1 g/L) and MnCl2 (3.6 mg/L). Shake flask fermentation were conducted at 30°C during 96 h with or without the supplementation of effectors according to a 23 factorial design. Under de novo lipid fermentation, lipid content was not significantly affected by fungal pelletization (p=0.315). The maximum lipid content was 32.38 ± 1.35 % of its defatted dry weight (w/w). However, the fatty acid (FA) profile obtaining from pellets (SFA: 19.57 %, PUFA: 36.43 %, MUFA: 44.00 %) was different to that obtaining form dispersed mycelium (SFA: 24.30 %, PUFA: 30.10 %, MUFA: 45.61 %). In addition, a significant interaction between FeCl3 and MnCl2 was detected (p<0.001). Under ex novo lipid fermentation, lipid content was significantly affected by the form of fungal growth (p=0.010). In this case, pelletization favored lipid accumulation. The maximum lipid content was 87.00 ± 1.41 % of its defatted dry weight (w/w). However, the FA profile obtaining from pellets (SFA: 22.01 %, PUFA: 19.92 %, MUFA: 58.07 %) was almost similar to that obtaining from dispersed mycelium (SFA: 21.19 %, PUFA: 22.82 %, MUFA: 55.99 %) and different to that observed from WCO (SFA: 12.11 %, PUFA: 48.32 %, MUFA: 38.31 %). In addition, the interaction between FeCl3 and MnCl2 was not significant (p=0.092). Thus, when the biotransformations swift from de novo to ex novo the FA profiles were changed toward synthesizing higher amounts of MUFA and lower amounts of PUFA.
publishDate 2023
dc.date.none.fl_str_mv 2023
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238632
Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues; LIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Rosario; Argentina; 2023; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238632
identifier_str_mv Biotransformations via de novo and ex novo pathways mediated by Aspergillus niger MYA 135: lipid accumulation from industrial residues; LIX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Rosario; Argentina; 2023; 1-2
CONICET Digital
CONICET
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dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
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