Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
- Autores
- Vallese, Harmonie Agostina; Colo, Georgina Pamela; Viera, Solange; Politi, Luis Enrique; German, Olga Lorena
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.
Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología - Materia
-
MÜLLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEINS
SPARC - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/201892
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Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse modelVallese, Harmonie AgostinaColo, Georgina PamelaViera, SolangePoliti, Luis EnriqueGerman, Olga LorenaMÜLLER GLIAL CELLSRD1EXTRACELLULAR MATRIX PROTEINSSPARChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista MedicinaKotsias, Basilio Aristidesde Vito, EduardoNarvaiz Kantor, IsabelLüthy, Isabel2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201892Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-2151669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:19Zoai:ri.conicet.gov.ar:11336/201892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:19.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
title |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
spellingShingle |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model Vallese, Harmonie Agostina MÜLLER GLIAL CELLS RD1 EXTRACELLULAR MATRIX PROTEINS SPARC |
title_short |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
title_full |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
title_fullStr |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
title_full_unstemmed |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
title_sort |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model |
dc.creator.none.fl_str_mv |
Vallese, Harmonie Agostina Colo, Georgina Pamela Viera, Solange Politi, Luis Enrique German, Olga Lorena |
author |
Vallese, Harmonie Agostina |
author_facet |
Vallese, Harmonie Agostina Colo, Georgina Pamela Viera, Solange Politi, Luis Enrique German, Olga Lorena |
author_role |
author |
author2 |
Colo, Georgina Pamela Viera, Solange Politi, Luis Enrique German, Olga Lorena |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Kotsias, Basilio Aristides de Vito, Eduardo Narvaiz Kantor, Isabel Lüthy, Isabel |
dc.subject.none.fl_str_mv |
MÜLLER GLIAL CELLS RD1 EXTRACELLULAR MATRIX PROTEINS SPARC |
topic |
MÜLLER GLIAL CELLS RD1 EXTRACELLULAR MATRIX PROTEINS SPARC |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality. Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Sociedad Argentina de Fisiología |
description |
Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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http://hdl.handle.net/11336/201892 Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-215 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/201892 |
identifier_str_mv |
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-215 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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Nacional |
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Fundación Revista Medicina |
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Fundación Revista Medicina |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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