Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model

Autores
Vallese, Harmonie Agostina; Colo, Georgina Pamela; Viera, Solange; Politi, Luis Enrique; German, Olga Lorena
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.
Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
MÜLLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEINS
SPARC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201892
Acceder
id CONICETDig_5158fdeef7a793ee29e3995b0b057ce2
oai_identifier_str oai:ri.conicet.gov.ar:11336/201892
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse modelVallese, Harmonie AgostinaColo, Georgina PamelaViera, SolangePoliti, Luis EnriqueGerman, Olga LorenaMÜLLER GLIAL CELLSRD1EXTRACELLULAR MATRIX PROTEINSSPARChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista MedicinaKotsias, Basilio Aristidesde Vito, EduardoNarvaiz Kantor, IsabelLüthy, Isabel2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201892Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-2151669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:02Zoai:ri.conicet.gov.ar:11336/201892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:02.7CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
title Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
spellingShingle Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
Vallese, Harmonie Agostina
MÜLLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEINS
SPARC
title_short Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
title_full Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
title_fullStr Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
title_full_unstemmed Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
title_sort Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model
dc.creator.none.fl_str_mv Vallese, Harmonie Agostina
Colo, Georgina Pamela
Viera, Solange
Politi, Luis Enrique
German, Olga Lorena
author Vallese, Harmonie Agostina
author_facet Vallese, Harmonie Agostina
Colo, Georgina Pamela
Viera, Solange
Politi, Luis Enrique
German, Olga Lorena
author_role author
author2 Colo, Georgina Pamela
Viera, Solange
Politi, Luis Enrique
German, Olga Lorena
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Kotsias, Basilio Aristides
de Vito, Eduardo
Narvaiz Kantor, Isabel
Lüthy, Isabel
dc.subject.none.fl_str_mv MÜLLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEINS
SPARC
topic MÜLLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEINS
SPARC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.
Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Viera, Solange. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Müller glial cells (MGC) are retina specialized cells, which promote photoreceptors (PHR) survival. We demonstrated that they are stem cells, whose regenerative potential and lamellipodia are decreased in the retinal degeneration mouse (rd1), compared to the wild type (wt). This suggests that the extracellular matrix (ECM) could be al- tered in rd1, affecting rd1 MGC morphology and functionality, and interfering with substrate adhesion and lamellipodia extensions, hence leading to an excessive number of neurons interacting with MGC. The aim of this work was to study rd1 ECM protein expres- sion and localization, and determine whether ECM pretreatment could restore the rd1 MGC morphology and functionality. Using mixed neuro-glial cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FAs) stained with paxillin. Also, rd1 mixed neuro-glial cultures were seeded on culture dishes previously treated or not with ECM-enriched condi- tioned medium (ECM-CM) obtained from Schwanomma (RN22) cell line, to analyze rd1 MGC morphology, FAs, proliferation and PHR survival (using BrdU and DAPI, respectively). Our results showed a decrease in osteonectin expression and both number and length of FAs along with an increase in fibrillary FN expression in rd1 MGC, when compared to the wt condition. Also, FAs showed cortical lo- cations and mature shape in rd1 compared to the wt. Noteworthy, ECM-CM pretreatment restored rd1 FAs and MGC lamellipodia ex- tensions, hence decreasing the ratio in number of neurons growing on MGC. This ECM pretreatment, also promoted rd1 MGC prolifer- ation and decreased PHR death. As a whole, these results suggest that rd1 neuro-glial cultures present alterations in EMC protein syn- thesis and/or secretion, and that ECM supplementation improves MGC morphology and functionality.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201892
Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-215
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201892
identifier_str_mv Extracellular matrix pretreatment improves morphology and functionality in Müller glial cells in a retinal degeneration mouse model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual De La Sociedad Argentina De Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 De La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 215-215
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782194725421056
score 12.982451

Publicaciones similares