Müller glial cells alterations in a retinal degeneration mouse model

Autores
Vallese, Harmonie Agostina; Colo, Georgina Pamela; Politi, Luis Enrique; German, Olga Lorena
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Müller glial cells (MGCs) are stem cells and promote photoreceptors (PHRs) survival in the retina. However, multiple injuries to the retina trigger “reactive gliosis,” which might lead to neuronal death. We previously demonstrated that MGC in rd1 mouse (a retina degeneration model) have early alterations in morphology and in reactive and stem cell markers; and stem cell markers are partially restored when rd1 MGC are co-cultured with wt neurons. This suggests that impaired neuro-glial crosstalk affects the stem cell potential of rd1 MGC. We now investigated whether alterations in the expression of extracellular matrix (ECM) proteins participate in rd1 impaired crosstalk. Using mixed neuro-glial (NG) cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FA) at 6 days in vitro. Also, rd1 mixed NG cultures were seeded on culture dishes previously treated with ECM-enriched conditioned medium (ECM-CM), to analyze rd1 MGC morphology, FAs, proliferation, and PHR survival (using BrdU and DAPI, respectively). In addition, rd1 mixed NG cultures were supplemented with conditioned medium obtained from wt mixed NG cultures (NG-CM), and conversely, wt NG cultures were supplemented with conditioned medium from rd1 cultures to analyze MGC morphology. Our results showed a decrease in osteonectin expression, a fibrillary FN expression, and a decreased number and length of FAs. Also, FAs cortical locations were different in rd1 and in wt MGC mixed NG cultures. Noteworthy, ECM-CM pretreatment restored rd1 MGC cytoplasmic extension and their FAs and promoted rd1 MGC proliferation, and decreased PHR death. Likewise, preliminary results showed that wt NG-CM supplementation in rd1 mixed cultures expanded MGC lamellipodia and increased PHR survival. These results suggest that rd1 MGC present alterations in EMC protein synthesis and/or secretion, and that wt ECM supplementation improves MGC morphology and functionality.
Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
First Meeting of the Glia Club Southern Cone
Buenos Aires
Argentina
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
Materia
MULLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEIN
SPARC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229298
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229298
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Müller glial cells alterations in a retinal degeneration mouse modelVallese, Harmonie AgostinaColo, Georgina PamelaPoliti, Luis EnriqueGerman, Olga LorenaMULLER GLIAL CELLSRD1EXTRACELLULAR MATRIX PROTEINSPARChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Müller glial cells (MGCs) are stem cells and promote photoreceptors (PHRs) survival in the retina. However, multiple injuries to the retina trigger “reactive gliosis,” which might lead to neuronal death. We previously demonstrated that MGC in rd1 mouse (a retina degeneration model) have early alterations in morphology and in reactive and stem cell markers; and stem cell markers are partially restored when rd1 MGC are co-cultured with wt neurons. This suggests that impaired neuro-glial crosstalk affects the stem cell potential of rd1 MGC. We now investigated whether alterations in the expression of extracellular matrix (ECM) proteins participate in rd1 impaired crosstalk. Using mixed neuro-glial (NG) cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FA) at 6 days in vitro. Also, rd1 mixed NG cultures were seeded on culture dishes previously treated with ECM-enriched conditioned medium (ECM-CM), to analyze rd1 MGC morphology, FAs, proliferation, and PHR survival (using BrdU and DAPI, respectively). In addition, rd1 mixed NG cultures were supplemented with conditioned medium obtained from wt mixed NG cultures (NG-CM), and conversely, wt NG cultures were supplemented with conditioned medium from rd1 cultures to analyze MGC morphology. Our results showed a decrease in osteonectin expression, a fibrillary FN expression, and a decreased number and length of FAs. Also, FAs cortical locations were different in rd1 and in wt MGC mixed NG cultures. Noteworthy, ECM-CM pretreatment restored rd1 MGC cytoplasmic extension and their FAs and promoted rd1 MGC proliferation, and decreased PHR death. Likewise, preliminary results showed that wt NG-CM supplementation in rd1 mixed cultures expanded MGC lamellipodia and increased PHR survival. These results suggest that rd1 MGC present alterations in EMC protein synthesis and/or secretion, and that wt ECM supplementation improves MGC morphology and functionality.Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFirst Meeting of the Glia Club Southern ConeBuenos AiresArgentinaUniversidad de Buenos Aires. Facultad de Farmacia y BioquímicaSAGE Publications2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229298Müller glial cells alterations in a retinal degeneration mouse model; First Meeting of the Glia Club Southern Cone; Buenos Aires; Argentina; 2022; 25-261759-09141759-0914CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/17590914221138206info:eu-repo/semantics/altIdentifier/doi/10.1177/17590914221138206Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:51Zoai:ri.conicet.gov.ar:11336/229298instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:51.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Müller glial cells alterations in a retinal degeneration mouse model
title Müller glial cells alterations in a retinal degeneration mouse model
spellingShingle Müller glial cells alterations in a retinal degeneration mouse model
Vallese, Harmonie Agostina
MULLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEIN
SPARC
title_short Müller glial cells alterations in a retinal degeneration mouse model
title_full Müller glial cells alterations in a retinal degeneration mouse model
title_fullStr Müller glial cells alterations in a retinal degeneration mouse model
title_full_unstemmed Müller glial cells alterations in a retinal degeneration mouse model
title_sort Müller glial cells alterations in a retinal degeneration mouse model
dc.creator.none.fl_str_mv Vallese, Harmonie Agostina
Colo, Georgina Pamela
Politi, Luis Enrique
German, Olga Lorena
author Vallese, Harmonie Agostina
author_facet Vallese, Harmonie Agostina
Colo, Georgina Pamela
Politi, Luis Enrique
German, Olga Lorena
author_role author
author2 Colo, Georgina Pamela
Politi, Luis Enrique
German, Olga Lorena
author2_role author
author
author
dc.subject.none.fl_str_mv MULLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEIN
SPARC
topic MULLER GLIAL CELLS
RD1
EXTRACELLULAR MATRIX PROTEIN
SPARC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Müller glial cells (MGCs) are stem cells and promote photoreceptors (PHRs) survival in the retina. However, multiple injuries to the retina trigger “reactive gliosis,” which might lead to neuronal death. We previously demonstrated that MGC in rd1 mouse (a retina degeneration model) have early alterations in morphology and in reactive and stem cell markers; and stem cell markers are partially restored when rd1 MGC are co-cultured with wt neurons. This suggests that impaired neuro-glial crosstalk affects the stem cell potential of rd1 MGC. We now investigated whether alterations in the expression of extracellular matrix (ECM) proteins participate in rd1 impaired crosstalk. Using mixed neuro-glial (NG) cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FA) at 6 days in vitro. Also, rd1 mixed NG cultures were seeded on culture dishes previously treated with ECM-enriched conditioned medium (ECM-CM), to analyze rd1 MGC morphology, FAs, proliferation, and PHR survival (using BrdU and DAPI, respectively). In addition, rd1 mixed NG cultures were supplemented with conditioned medium obtained from wt mixed NG cultures (NG-CM), and conversely, wt NG cultures were supplemented with conditioned medium from rd1 cultures to analyze MGC morphology. Our results showed a decrease in osteonectin expression, a fibrillary FN expression, and a decreased number and length of FAs. Also, FAs cortical locations were different in rd1 and in wt MGC mixed NG cultures. Noteworthy, ECM-CM pretreatment restored rd1 MGC cytoplasmic extension and their FAs and promoted rd1 MGC proliferation, and decreased PHR death. Likewise, preliminary results showed that wt NG-CM supplementation in rd1 mixed cultures expanded MGC lamellipodia and increased PHR survival. These results suggest that rd1 MGC present alterations in EMC protein synthesis and/or secretion, and that wt ECM supplementation improves MGC morphology and functionality.
Fil: Vallese, Harmonie Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
First Meeting of the Glia Club Southern Cone
Buenos Aires
Argentina
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
description Müller glial cells (MGCs) are stem cells and promote photoreceptors (PHRs) survival in the retina. However, multiple injuries to the retina trigger “reactive gliosis,” which might lead to neuronal death. We previously demonstrated that MGC in rd1 mouse (a retina degeneration model) have early alterations in morphology and in reactive and stem cell markers; and stem cell markers are partially restored when rd1 MGC are co-cultured with wt neurons. This suggests that impaired neuro-glial crosstalk affects the stem cell potential of rd1 MGC. We now investigated whether alterations in the expression of extracellular matrix (ECM) proteins participate in rd1 impaired crosstalk. Using mixed neuro-glial (NG) cultures obtained from postnatal 2 days rd1 and wt mice retinas, we analyzed by immunocytochemistry, osteonectin and fibronectin (FN) expression and focal adhesions (FA) at 6 days in vitro. Also, rd1 mixed NG cultures were seeded on culture dishes previously treated with ECM-enriched conditioned medium (ECM-CM), to analyze rd1 MGC morphology, FAs, proliferation, and PHR survival (using BrdU and DAPI, respectively). In addition, rd1 mixed NG cultures were supplemented with conditioned medium obtained from wt mixed NG cultures (NG-CM), and conversely, wt NG cultures were supplemented with conditioned medium from rd1 cultures to analyze MGC morphology. Our results showed a decrease in osteonectin expression, a fibrillary FN expression, and a decreased number and length of FAs. Also, FAs cortical locations were different in rd1 and in wt MGC mixed NG cultures. Noteworthy, ECM-CM pretreatment restored rd1 MGC cytoplasmic extension and their FAs and promoted rd1 MGC proliferation, and decreased PHR death. Likewise, preliminary results showed that wt NG-CM supplementation in rd1 mixed cultures expanded MGC lamellipodia and increased PHR survival. These results suggest that rd1 MGC present alterations in EMC protein synthesis and/or secretion, and that wt ECM supplementation improves MGC morphology and functionality.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229298
Müller glial cells alterations in a retinal degeneration mouse model; First Meeting of the Glia Club Southern Cone; Buenos Aires; Argentina; 2022; 25-26
1759-0914
1759-0914
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229298
identifier_str_mv Müller glial cells alterations in a retinal degeneration mouse model; First Meeting of the Glia Club Southern Cone; Buenos Aires; Argentina; 2022; 25-26
1759-0914
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/17590914221138206
info:eu-repo/semantics/altIdentifier/doi/10.1177/17590914221138206
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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