Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms

Autores
Arias, Jose Luis; Callegari, Eduardo Alberto; Paez, Daniela Veronica; Rodrigo, Blas; Munarriz, Alicia; Pardo Hidalgo, Rodolfo; Tamashiro, Hector; Di Genaro, Maria Silvia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
A role for intestinal microbes in arthritis is being considered. We analyzed intra-articular microbiota components and their association with immunopathogenic mechanisms of Spondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled (n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approved CE002-2017). IL-17, IL-6, IL-23 and TGF-b levels were quantified in each SF sample by ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells (human synovial fibroblasts) were incubated with the SpA SF pool in absence or presence of polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant were measured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in the SpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study. Higher levels of IL-17, IL-6, IL-23 and TGF-b were detected in SpA compared with OA SF (p<0.001, p<0.01, p<0.001, and p<0.01, respectively). Since segmented filamentous bacteria (SFB) have a cardinal feature to induce Th17 cell differentiation in gut immunity, we searched for SFB peptides in the SF of SpA. We found 54 peptides of SFB with abundance estimated with an exponentially modified Protein Abundance Index (emPAI) from 0.01 to 0.28. In addition, LPS is present in synovial microenvironment of SpA since polymyxin B treatment significantly reduced IL-6 secretion by synovial fibroblasts stimulated with SpA SF (p<0.05). Therefore, we explored the synovial presence of peptides of microbiota Gram-negative bacteria, particularly genus Dialister because it was described as a potential intestinal microbial marker of disease activity in SpA. Accordingly, we found 55 peptides of genus Dialister (emPAI 0.02-0.33). Our findings show the presence of LPS and microbiota bacterial proteins in the synovial microenvironment of peripheral SpA and suggest their potential association with immunopathogenic mechanisms of this inflammatory arthropathy.
Fil: Arias, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Callegari, Eduardo Alberto. University Of South Dakota. Sanford School Of Medicine.; Estados Unidos
Fil: Paez, Daniela Veronica. University Of South Dakota. Sanford School Of Medicine.; Estados Unidos
Fil: Rodrigo, Blas. Centro Médico Medici; Argentina
Fil: Munarriz, Alicia. Centro Médico CENYR; Argentina
Fil: Pardo Hidalgo, Rodolfo. Centro de Rehabilitación Médica CER; Argentina
Fil: Tamashiro, Hector. Centro Médico Bolívar; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas
Buenos Aires
Argentina
Asociación Argentina de de Nanomedicinas
Sociedad Argentina de Investigación Clinica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Materia
PERIPHERAL SPONDYLOARTHRITIS
MICROBIOTA
SYNOVIAL FLUID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190391
Acceder
id CONICETDig_512eb833e973289d05957c031aa6d47d
oai_identifier_str oai:ri.conicet.gov.ar:11336/190391
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanismsArias, Jose LuisCallegari, Eduardo AlbertoPaez, Daniela VeronicaRodrigo, BlasMunarriz, AliciaPardo Hidalgo, RodolfoTamashiro, HectorDi Genaro, Maria SilviaPERIPHERAL SPONDYLOARTHRITISMICROBIOTASYNOVIAL FLUIDhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3A role for intestinal microbes in arthritis is being considered. We analyzed intra-articular microbiota components and their association with immunopathogenic mechanisms of Spondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled (n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approved CE002-2017). IL-17, IL-6, IL-23 and TGF-b levels were quantified in each SF sample by ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells (human synovial fibroblasts) were incubated with the SpA SF pool in absence or presence of polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant were measured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in the SpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study. Higher levels of IL-17, IL-6, IL-23 and TGF-b were detected in SpA compared with OA SF (p<0.001, p<0.01, p<0.001, and p<0.01, respectively). Since segmented filamentous bacteria (SFB) have a cardinal feature to induce Th17 cell differentiation in gut immunity, we searched for SFB peptides in the SF of SpA. We found 54 peptides of SFB with abundance estimated with an exponentially modified Protein Abundance Index (emPAI) from 0.01 to 0.28. In addition, LPS is present in synovial microenvironment of SpA since polymyxin B treatment significantly reduced IL-6 secretion by synovial fibroblasts stimulated with SpA SF (p<0.05). Therefore, we explored the synovial presence of peptides of microbiota Gram-negative bacteria, particularly genus Dialister because it was described as a potential intestinal microbial marker of disease activity in SpA. Accordingly, we found 55 peptides of genus Dialister (emPAI 0.02-0.33). Our findings show the presence of LPS and microbiota bacterial proteins in the synovial microenvironment of peripheral SpA and suggest their potential association with immunopathogenic mechanisms of this inflammatory arthropathy.Fil: Arias, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Callegari, Eduardo Alberto. University Of South Dakota. Sanford School Of Medicine.; Estados UnidosFil: Paez, Daniela Veronica. University Of South Dakota. Sanford School Of Medicine.; Estados UnidosFil: Rodrigo, Blas. Centro Médico Medici; ArgentinaFil: Munarriz, Alicia. Centro Médico CENYR; ArgentinaFil: Pardo Hidalgo, Rodolfo. Centro de Rehabilitación Médica CER; ArgentinaFil: Tamashiro, Hector. Centro Médico Bolívar; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaLXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de NanomedicinasBuenos AiresArgentinaAsociación Argentina de de NanomedicinasSociedad Argentina de Investigación ClinicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalFundación Revista MedicinaCurino, Alejandro CarlosMaccioni, MarianaSchaiquevich, Paula SusanaDuran, Hebe Alicia2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190391Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Buenos Aires; Argentina; 2021; 157-1580025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfinfo:eu-repo/semantics/altIdentifier/url/https://cedie.conicet.gov.ar/resumenes-saic-2021/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:56Zoai:ri.conicet.gov.ar:11336/190391instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:56.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
title Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
spellingShingle Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
Arias, Jose Luis
PERIPHERAL SPONDYLOARTHRITIS
MICROBIOTA
SYNOVIAL FLUID
title_short Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
title_full Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
title_fullStr Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
title_full_unstemmed Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
title_sort Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms
dc.creator.none.fl_str_mv Arias, Jose Luis
Callegari, Eduardo Alberto
Paez, Daniela Veronica
Rodrigo, Blas
Munarriz, Alicia
Pardo Hidalgo, Rodolfo
Tamashiro, Hector
Di Genaro, Maria Silvia
author Arias, Jose Luis
author_facet Arias, Jose Luis
Callegari, Eduardo Alberto
Paez, Daniela Veronica
Rodrigo, Blas
Munarriz, Alicia
Pardo Hidalgo, Rodolfo
Tamashiro, Hector
Di Genaro, Maria Silvia
author_role author
author2 Callegari, Eduardo Alberto
Paez, Daniela Veronica
Rodrigo, Blas
Munarriz, Alicia
Pardo Hidalgo, Rodolfo
Tamashiro, Hector
Di Genaro, Maria Silvia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Curino, Alejandro Carlos
Maccioni, Mariana
Schaiquevich, Paula Susana
Duran, Hebe Alicia
dc.subject.none.fl_str_mv PERIPHERAL SPONDYLOARTHRITIS
MICROBIOTA
SYNOVIAL FLUID
topic PERIPHERAL SPONDYLOARTHRITIS
MICROBIOTA
SYNOVIAL FLUID
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A role for intestinal microbes in arthritis is being considered. We analyzed intra-articular microbiota components and their association with immunopathogenic mechanisms of Spondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled (n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approved CE002-2017). IL-17, IL-6, IL-23 and TGF-b levels were quantified in each SF sample by ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells (human synovial fibroblasts) were incubated with the SpA SF pool in absence or presence of polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant were measured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in the SpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study. Higher levels of IL-17, IL-6, IL-23 and TGF-b were detected in SpA compared with OA SF (p<0.001, p<0.01, p<0.001, and p<0.01, respectively). Since segmented filamentous bacteria (SFB) have a cardinal feature to induce Th17 cell differentiation in gut immunity, we searched for SFB peptides in the SF of SpA. We found 54 peptides of SFB with abundance estimated with an exponentially modified Protein Abundance Index (emPAI) from 0.01 to 0.28. In addition, LPS is present in synovial microenvironment of SpA since polymyxin B treatment significantly reduced IL-6 secretion by synovial fibroblasts stimulated with SpA SF (p<0.05). Therefore, we explored the synovial presence of peptides of microbiota Gram-negative bacteria, particularly genus Dialister because it was described as a potential intestinal microbial marker of disease activity in SpA. Accordingly, we found 55 peptides of genus Dialister (emPAI 0.02-0.33). Our findings show the presence of LPS and microbiota bacterial proteins in the synovial microenvironment of peripheral SpA and suggest their potential association with immunopathogenic mechanisms of this inflammatory arthropathy.
Fil: Arias, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Callegari, Eduardo Alberto. University Of South Dakota. Sanford School Of Medicine.; Estados Unidos
Fil: Paez, Daniela Veronica. University Of South Dakota. Sanford School Of Medicine.; Estados Unidos
Fil: Rodrigo, Blas. Centro Médico Medici; Argentina
Fil: Munarriz, Alicia. Centro Médico CENYR; Argentina
Fil: Pardo Hidalgo, Rodolfo. Centro de Rehabilitación Médica CER; Argentina
Fil: Tamashiro, Hector. Centro Médico Bolívar; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas
Buenos Aires
Argentina
Asociación Argentina de de Nanomedicinas
Sociedad Argentina de Investigación Clinica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
description A role for intestinal microbes in arthritis is being considered. We analyzed intra-articular microbiota components and their association with immunopathogenic mechanisms of Spondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled (n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approved CE002-2017). IL-17, IL-6, IL-23 and TGF-b levels were quantified in each SF sample by ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells (human synovial fibroblasts) were incubated with the SpA SF pool in absence or presence of polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant were measured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in the SpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study. Higher levels of IL-17, IL-6, IL-23 and TGF-b were detected in SpA compared with OA SF (p<0.001, p<0.01, p<0.001, and p<0.01, respectively). Since segmented filamentous bacteria (SFB) have a cardinal feature to induce Th17 cell differentiation in gut immunity, we searched for SFB peptides in the SF of SpA. We found 54 peptides of SFB with abundance estimated with an exponentially modified Protein Abundance Index (emPAI) from 0.01 to 0.28. In addition, LPS is present in synovial microenvironment of SpA since polymyxin B treatment significantly reduced IL-6 secretion by synovial fibroblasts stimulated with SpA SF (p<0.05). Therefore, we explored the synovial presence of peptides of microbiota Gram-negative bacteria, particularly genus Dialister because it was described as a potential intestinal microbial marker of disease activity in SpA. Accordingly, we found 55 peptides of genus Dialister (emPAI 0.02-0.33). Our findings show the presence of LPS and microbiota bacterial proteins in the synovial microenvironment of peripheral SpA and suggest their potential association with immunopathogenic mechanisms of this inflammatory arthropathy.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190391
Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Buenos Aires; Argentina; 2021; 157-158
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190391
identifier_str_mv Detection of intestinal microbiota components in the synovial microenviroment of peripheral spondyloarthritis and its association to immunopathogenic mechanisms; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental y XI Reunión Anual de la Asociación Argentina de Nanomedicinas; Buenos Aires; Argentina; 2021; 157-158
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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