Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway dist...
- Autores
- Teves, Franco; Lamas Maceiras, Mónica; García Estrada, Carlos; Casqueiro, Javier; Naranjo, Leopoldo; Ullán, Ricardo V.; Scervino, Jose Martin; Wu, Xiaobin; Velasco Conde, Tania; Martín, Juan F.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The lysine biosynthetic pathway has to supply large amounts of α-aminoadipic acid for penicillin biosynthesis in Penicillium chrysogenum. In this study, we have characterized the P. chrysogenum L2 mutant, a lysine auxotroph that shows highly increased expression of several lysine biosynthesis genes (lys1, lys2, lys3, lys7). The L2 mutant was found to be deficient in homoaconitase activity since it was complemented by the Aspergillus nidulans lysF gene. We have cloned a gene (named lys3) that complements the L2 mutation by transformation with a P. chrysogenum genomic library, constructed in an autonomous replicating plasmid. The lys3-encoded protein showed high identity to homoaconitases. In addition, we cloned the mutant lys3 allele from the L2 strain that showed a G1534 to A1534 point mutation resulting in a Gly495 to Asp495 substitution. This mutation is located in a highly conserved region adjacent to two of the three cysteine residues that act as ligands to bind the iron-sulfur cluster required for homoaconitase activity. The L2 mutant accumulates homocitrate. Deletion of the lys1 gene (homocitrate synthase) in the L2 strain prevented homocitrate accumulation and reverted expression levels of the four lysine biosynthesis genes tested to those of the parental prototrophic strain. Homocitrate accumulation seems to act as a sensor of lysine-pathway distress, triggering overexpression of four of the lysine biosynthesis genes.
Fil: Teves, Franco. Universidad de León; España
Fil: Lamas Maceiras, Mónica. Universidad de León; España
Fil: García Estrada, Carlos. Instituto de Biotecnología de León; España
Fil: Casqueiro, Javier. Instituto de Biotecnología de León; España. Universidad de León; España
Fil: Naranjo, Leopoldo. Universidad de León; España
Fil: Ullán, Ricardo V.. Instituto de Biotecnología de León; España
Fil: Scervino, Jose Martin. Instituto de Biotecnología de León; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Wu, Xiaobin. Instituto de Biotecnología de León; España
Fil: Velasco Conde, Tania. Instituto de Biotecnología de León; España
Fil: Martín, Juan F.. Instituto de Biotecnología de León; España. Universidad de León; España - Materia
- Penicilin
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/125462
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oai:ri.conicet.gov.ar:11336/125462 |
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repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
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Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distressTeves, FrancoLamas Maceiras, MónicaGarcía Estrada, CarlosCasqueiro, JavierNaranjo, LeopoldoUllán, Ricardo V.Scervino, Jose MartinWu, XiaobinVelasco Conde, TaniaMartín, Juan F.Penicilinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The lysine biosynthetic pathway has to supply large amounts of α-aminoadipic acid for penicillin biosynthesis in Penicillium chrysogenum. In this study, we have characterized the P. chrysogenum L2 mutant, a lysine auxotroph that shows highly increased expression of several lysine biosynthesis genes (lys1, lys2, lys3, lys7). The L2 mutant was found to be deficient in homoaconitase activity since it was complemented by the Aspergillus nidulans lysF gene. We have cloned a gene (named lys3) that complements the L2 mutation by transformation with a P. chrysogenum genomic library, constructed in an autonomous replicating plasmid. The lys3-encoded protein showed high identity to homoaconitases. In addition, we cloned the mutant lys3 allele from the L2 strain that showed a G1534 to A1534 point mutation resulting in a Gly495 to Asp495 substitution. This mutation is located in a highly conserved region adjacent to two of the three cysteine residues that act as ligands to bind the iron-sulfur cluster required for homoaconitase activity. The L2 mutant accumulates homocitrate. Deletion of the lys1 gene (homocitrate synthase) in the L2 strain prevented homocitrate accumulation and reverted expression levels of the four lysine biosynthesis genes tested to those of the parental prototrophic strain. Homocitrate accumulation seems to act as a sensor of lysine-pathway distress, triggering overexpression of four of the lysine biosynthesis genes.Fil: Teves, Franco. Universidad de León; EspañaFil: Lamas Maceiras, Mónica. Universidad de León; EspañaFil: García Estrada, Carlos. Instituto de Biotecnología de León; EspañaFil: Casqueiro, Javier. Instituto de Biotecnología de León; España. Universidad de León; EspañaFil: Naranjo, Leopoldo. Universidad de León; EspañaFil: Ullán, Ricardo V.. Instituto de Biotecnología de León; EspañaFil: Scervino, Jose Martin. Instituto de Biotecnología de León; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Wu, Xiaobin. Instituto de Biotecnología de León; EspañaFil: Velasco Conde, Tania. Instituto de Biotecnología de León; EspañaFil: Martín, Juan F.. Instituto de Biotecnología de León; España. Universidad de León; EspañaSociety for General Microbiology2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125462Teves, Franco; Lamas Maceiras, Mónica; García Estrada, Carlos; Casqueiro, Javier; Naranjo, Leopoldo; et al.; Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress; Society for General Microbiology; Microbiology-UK; 155; 12; 12-2009; 3881-38921350-0872CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.031005-0#tab2info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.031005-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:08Zoai:ri.conicet.gov.ar:11336/125462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:09.199CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
title |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
spellingShingle |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress Teves, Franco Penicilin |
title_short |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
title_full |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
title_fullStr |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
title_full_unstemmed |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
title_sort |
Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress |
dc.creator.none.fl_str_mv |
Teves, Franco Lamas Maceiras, Mónica García Estrada, Carlos Casqueiro, Javier Naranjo, Leopoldo Ullán, Ricardo V. Scervino, Jose Martin Wu, Xiaobin Velasco Conde, Tania Martín, Juan F. |
author |
Teves, Franco |
author_facet |
Teves, Franco Lamas Maceiras, Mónica García Estrada, Carlos Casqueiro, Javier Naranjo, Leopoldo Ullán, Ricardo V. Scervino, Jose Martin Wu, Xiaobin Velasco Conde, Tania Martín, Juan F. |
author_role |
author |
author2 |
Lamas Maceiras, Mónica García Estrada, Carlos Casqueiro, Javier Naranjo, Leopoldo Ullán, Ricardo V. Scervino, Jose Martin Wu, Xiaobin Velasco Conde, Tania Martín, Juan F. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Penicilin |
topic |
Penicilin |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The lysine biosynthetic pathway has to supply large amounts of α-aminoadipic acid for penicillin biosynthesis in Penicillium chrysogenum. In this study, we have characterized the P. chrysogenum L2 mutant, a lysine auxotroph that shows highly increased expression of several lysine biosynthesis genes (lys1, lys2, lys3, lys7). The L2 mutant was found to be deficient in homoaconitase activity since it was complemented by the Aspergillus nidulans lysF gene. We have cloned a gene (named lys3) that complements the L2 mutation by transformation with a P. chrysogenum genomic library, constructed in an autonomous replicating plasmid. The lys3-encoded protein showed high identity to homoaconitases. In addition, we cloned the mutant lys3 allele from the L2 strain that showed a G1534 to A1534 point mutation resulting in a Gly495 to Asp495 substitution. This mutation is located in a highly conserved region adjacent to two of the three cysteine residues that act as ligands to bind the iron-sulfur cluster required for homoaconitase activity. The L2 mutant accumulates homocitrate. Deletion of the lys1 gene (homocitrate synthase) in the L2 strain prevented homocitrate accumulation and reverted expression levels of the four lysine biosynthesis genes tested to those of the parental prototrophic strain. Homocitrate accumulation seems to act as a sensor of lysine-pathway distress, triggering overexpression of four of the lysine biosynthesis genes. Fil: Teves, Franco. Universidad de León; España Fil: Lamas Maceiras, Mónica. Universidad de León; España Fil: García Estrada, Carlos. Instituto de Biotecnología de León; España Fil: Casqueiro, Javier. Instituto de Biotecnología de León; España. Universidad de León; España Fil: Naranjo, Leopoldo. Universidad de León; España Fil: Ullán, Ricardo V.. Instituto de Biotecnología de León; España Fil: Scervino, Jose Martin. Instituto de Biotecnología de León; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Wu, Xiaobin. Instituto de Biotecnología de León; España Fil: Velasco Conde, Tania. Instituto de Biotecnología de León; España Fil: Martín, Juan F.. Instituto de Biotecnología de León; España. Universidad de León; España |
description |
The lysine biosynthetic pathway has to supply large amounts of α-aminoadipic acid for penicillin biosynthesis in Penicillium chrysogenum. In this study, we have characterized the P. chrysogenum L2 mutant, a lysine auxotroph that shows highly increased expression of several lysine biosynthesis genes (lys1, lys2, lys3, lys7). The L2 mutant was found to be deficient in homoaconitase activity since it was complemented by the Aspergillus nidulans lysF gene. We have cloned a gene (named lys3) that complements the L2 mutation by transformation with a P. chrysogenum genomic library, constructed in an autonomous replicating plasmid. The lys3-encoded protein showed high identity to homoaconitases. In addition, we cloned the mutant lys3 allele from the L2 strain that showed a G1534 to A1534 point mutation resulting in a Gly495 to Asp495 substitution. This mutation is located in a highly conserved region adjacent to two of the three cysteine residues that act as ligands to bind the iron-sulfur cluster required for homoaconitase activity. The L2 mutant accumulates homocitrate. Deletion of the lys1 gene (homocitrate synthase) in the L2 strain prevented homocitrate accumulation and reverted expression levels of the four lysine biosynthesis genes tested to those of the parental prototrophic strain. Homocitrate accumulation seems to act as a sensor of lysine-pathway distress, triggering overexpression of four of the lysine biosynthesis genes. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/125462 Teves, Franco; Lamas Maceiras, Mónica; García Estrada, Carlos; Casqueiro, Javier; Naranjo, Leopoldo; et al.; Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress; Society for General Microbiology; Microbiology-UK; 155; 12; 12-2009; 3881-3892 1350-0872 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/125462 |
identifier_str_mv |
Teves, Franco; Lamas Maceiras, Mónica; García Estrada, Carlos; Casqueiro, Javier; Naranjo, Leopoldo; et al.; Transcriptional upregulation of four genes of the lysine biosynthetic pathway by homocitrate accumulation in Penicillium chrysogenum: homocitrate as a sensor of lysine-pathway distress; Society for General Microbiology; Microbiology-UK; 155; 12; 12-2009; 3881-3892 1350-0872 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.031005-0#tab2 info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.031005-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for General Microbiology |
publisher.none.fl_str_mv |
Society for General Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613664937607168 |
score |
13.070432 |