Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms

Autores
Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.
Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; Austria
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
GRAPHENE FIELD EFFECT TRANSISTORS
GLUCOSE
POLYANILINES
GLUCOSE OXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/171646
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/171646
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilmsFenoy, Gonzalo EduardoMarmisollé, Waldemar AlejandroKnoll, WolfgangAzzaroni, OmarGRAPHENE FIELD EFFECT TRANSISTORSGLUCOSEPOLYANILINESGLUCOSE OXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; AustriaFil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaRoyal Society of Chemistry2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171646Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-1482635-0998CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D1SD00007Ainfo:eu-repo/semantics/altIdentifier/doi/10.1039/D1SD00007Ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:38:55Zoai:ri.conicet.gov.ar:11336/171646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:38:55.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
title Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
spellingShingle Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
Fenoy, Gonzalo Eduardo
GRAPHENE FIELD EFFECT TRANSISTORS
GLUCOSE
POLYANILINES
GLUCOSE OXIDASE
title_short Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
title_full Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
title_fullStr Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
title_full_unstemmed Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
title_sort Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
dc.creator.none.fl_str_mv Fenoy, Gonzalo Eduardo
Marmisollé, Waldemar Alejandro
Knoll, Wolfgang
Azzaroni, Omar
author Fenoy, Gonzalo Eduardo
author_facet Fenoy, Gonzalo Eduardo
Marmisollé, Waldemar Alejandro
Knoll, Wolfgang
Azzaroni, Omar
author_role author
author2 Marmisollé, Waldemar Alejandro
Knoll, Wolfgang
Azzaroni, Omar
author2_role author
author
author
dc.subject.none.fl_str_mv GRAPHENE FIELD EFFECT TRANSISTORS
GLUCOSE
POLYANILINES
GLUCOSE OXIDASE
topic GRAPHENE FIELD EFFECT TRANSISTORS
GLUCOSE
POLYANILINES
GLUCOSE OXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.
Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; Austria
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/171646
Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-148
2635-0998
CONICET Digital
CONICET
url http://hdl.handle.net/11336/171646
identifier_str_mv Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-148
2635-0998
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D1SD00007A
info:eu-repo/semantics/altIdentifier/doi/10.1039/D1SD00007A
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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