Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms
- Autores
- Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.
Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; Austria
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina - Materia
-
GRAPHENE FIELD EFFECT TRANSISTORS
GLUCOSE
POLYANILINES
GLUCOSE OXIDASE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/171646
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilmsFenoy, Gonzalo EduardoMarmisollé, Waldemar AlejandroKnoll, WolfgangAzzaroni, OmarGRAPHENE FIELD EFFECT TRANSISTORSGLUCOSEPOLYANILINESGLUCOSE OXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices.Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; AustriaFil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaRoyal Society of Chemistry2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171646Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-1482635-0998CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D1SD00007Ainfo:eu-repo/semantics/altIdentifier/doi/10.1039/D1SD00007Ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:03Zoai:ri.conicet.gov.ar:11336/171646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:03.945CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
title |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
spellingShingle |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms Fenoy, Gonzalo Eduardo GRAPHENE FIELD EFFECT TRANSISTORS GLUCOSE POLYANILINES GLUCOSE OXIDASE |
title_short |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
title_full |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
title_fullStr |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
title_full_unstemmed |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
title_sort |
Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms |
dc.creator.none.fl_str_mv |
Fenoy, Gonzalo Eduardo Marmisollé, Waldemar Alejandro Knoll, Wolfgang Azzaroni, Omar |
author |
Fenoy, Gonzalo Eduardo |
author_facet |
Fenoy, Gonzalo Eduardo Marmisollé, Waldemar Alejandro Knoll, Wolfgang Azzaroni, Omar |
author_role |
author |
author2 |
Marmisollé, Waldemar Alejandro Knoll, Wolfgang Azzaroni, Omar |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
GRAPHENE FIELD EFFECT TRANSISTORS GLUCOSE POLYANILINES GLUCOSE OXIDASE |
topic |
GRAPHENE FIELD EFFECT TRANSISTORS GLUCOSE POLYANILINES GLUCOSE OXIDASE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices. Fil: Fenoy, Gonzalo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Knoll, Wolfgang. AIT Austrian Institute of Technology; Austria. Danube Private University; Austria Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina |
description |
We introduce a new approach for glucose oxidase (GOx) immobilization on graphene field-effect transistors (gFETs) to fabricate highly sensitive glucose sensors. The strategy relies on the electropolymerization of a layer of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) on graphene-based transistors. The synthesized polymer film provides the suitable electrostatic charge and non-denaturing environment for enzyme immobilization without the need of any chemical primer. Then, the local pH changes triggered by the enzyme-catalyzed oxidation produce a shift in the Dirac potential of the gFETs to more negative values, which is evidenced by the differences in the graphene channel conductivity and constitutes the signal transduction mechanism of the sensing devices. The assembled biosensors revealed a low LOD of 4.1 μM and were capable of detecting glucose in the range from 10 to 1000 μM in a flow configuration. Moreover, they showed sensitivity of −24.9 μA per decade of glucose concentration and a fast response time, with an average value of 190 seconds, while allowing the operation at small gate-source and drain-source voltages. Finally, the biosensors were able to successfully monitor the analyte in urine samples, showing their potential towards the fabrication of point-of-care glucose testing devices. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/171646 Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-148 2635-0998 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/171646 |
identifier_str_mv |
Fenoy, Gonzalo Eduardo; Marmisollé, Waldemar Alejandro; Knoll, Wolfgang; Azzaroni, Omar; Highly sensitive urine glucose detection with graphene field-effect transistors functionalized with electropolymerized nanofilms; Royal Society of Chemistry; Sensors & Diagnostics; 1; 1; 10-2021; 139-148 2635-0998 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D1SD00007A info:eu-repo/semantics/altIdentifier/doi/10.1039/D1SD00007A |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613964857606144 |
score |
13.070432 |