Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection

Autores
Calzadilla, Pablo Ignacio; Song, Junliang; Gallois, Patrick; Johnson, Giles Nicholas
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Plastid Terminal Oxidase (PTOX) is a chloroplast localized plastoquinone oxygen oxidoreductase suggested to have the potential to act as a photoprotective safety valve for photosynthesis. However, PTOX overexpression in plants has been unsuccessful at inducing photoprotection, and the factors that control its activity remain elusive. Here, we show that significant PTOX activity is induced in response to high light in the model species Eutrema salsugineum and Arabidopsis thaliana. This activation correlates with structural reorganization of the thylakoid membrane. Over-expression of PTOX in mutants of Arabidopsis thaliana perturbed in thylakoid stacking also results in such activity, in contrast to wild type plants with normal granal structure. Further, PTOX activation protects against photoinhibition of Photosystem II and reduces reactive oxygen production under stress conditions. We conclude that structural re-arrangements of the thylakoid membranes, bringing Photosystem II and PTOX into proximity, are both required and sufficient for PTOX to act as a Photosystem II sink and play a role in photoprotection.
Fil: Calzadilla, Pablo Ignacio. University of Manchester; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Song, Junliang. University of Manchester; Reino Unido
Fil: Gallois, Patrick. University of Manchester; Reino Unido
Fil: Johnson, Giles Nicholas. University of Manchester; Reino Unido
Materia
PHOTOSYNTHESIS
ELECTRON TRANSPORT
STRESS
EUTREMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/264573
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotectionCalzadilla, Pablo IgnacioSong, JunliangGallois, PatrickJohnson, Giles NicholasPHOTOSYNTHESISELECTRON TRANSPORTSTRESSEUTREMAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Plastid Terminal Oxidase (PTOX) is a chloroplast localized plastoquinone oxygen oxidoreductase suggested to have the potential to act as a photoprotective safety valve for photosynthesis. However, PTOX overexpression in plants has been unsuccessful at inducing photoprotection, and the factors that control its activity remain elusive. Here, we show that significant PTOX activity is induced in response to high light in the model species Eutrema salsugineum and Arabidopsis thaliana. This activation correlates with structural reorganization of the thylakoid membrane. Over-expression of PTOX in mutants of Arabidopsis thaliana perturbed in thylakoid stacking also results in such activity, in contrast to wild type plants with normal granal structure. Further, PTOX activation protects against photoinhibition of Photosystem II and reduces reactive oxygen production under stress conditions. We conclude that structural re-arrangements of the thylakoid membranes, bringing Photosystem II and PTOX into proximity, are both required and sufficient for PTOX to act as a Photosystem II sink and play a role in photoprotection.Fil: Calzadilla, Pablo Ignacio. University of Manchester; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Song, Junliang. University of Manchester; Reino UnidoFil: Gallois, Patrick. University of Manchester; Reino UnidoFil: Johnson, Giles Nicholas. University of Manchester; Reino UnidoSpringer2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264573Calzadilla, Pablo Ignacio; Song, Junliang; Gallois, Patrick; Johnson, Giles Nicholas; Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection; Springer; Nature Communications; 15; 1; 1-2024; 1-132041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-023-44454-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-023-44454-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:31:44Zoai:ri.conicet.gov.ar:11336/264573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:31:44.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
title Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
spellingShingle Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
Calzadilla, Pablo Ignacio
PHOTOSYNTHESIS
ELECTRON TRANSPORT
STRESS
EUTREMA
title_short Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
title_full Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
title_fullStr Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
title_full_unstemmed Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
title_sort Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection
dc.creator.none.fl_str_mv Calzadilla, Pablo Ignacio
Song, Junliang
Gallois, Patrick
Johnson, Giles Nicholas
author Calzadilla, Pablo Ignacio
author_facet Calzadilla, Pablo Ignacio
Song, Junliang
Gallois, Patrick
Johnson, Giles Nicholas
author_role author
author2 Song, Junliang
Gallois, Patrick
Johnson, Giles Nicholas
author2_role author
author
author
dc.subject.none.fl_str_mv PHOTOSYNTHESIS
ELECTRON TRANSPORT
STRESS
EUTREMA
topic PHOTOSYNTHESIS
ELECTRON TRANSPORT
STRESS
EUTREMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Plastid Terminal Oxidase (PTOX) is a chloroplast localized plastoquinone oxygen oxidoreductase suggested to have the potential to act as a photoprotective safety valve for photosynthesis. However, PTOX overexpression in plants has been unsuccessful at inducing photoprotection, and the factors that control its activity remain elusive. Here, we show that significant PTOX activity is induced in response to high light in the model species Eutrema salsugineum and Arabidopsis thaliana. This activation correlates with structural reorganization of the thylakoid membrane. Over-expression of PTOX in mutants of Arabidopsis thaliana perturbed in thylakoid stacking also results in such activity, in contrast to wild type plants with normal granal structure. Further, PTOX activation protects against photoinhibition of Photosystem II and reduces reactive oxygen production under stress conditions. We conclude that structural re-arrangements of the thylakoid membranes, bringing Photosystem II and PTOX into proximity, are both required and sufficient for PTOX to act as a Photosystem II sink and play a role in photoprotection.
Fil: Calzadilla, Pablo Ignacio. University of Manchester; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Song, Junliang. University of Manchester; Reino Unido
Fil: Gallois, Patrick. University of Manchester; Reino Unido
Fil: Johnson, Giles Nicholas. University of Manchester; Reino Unido
description The Plastid Terminal Oxidase (PTOX) is a chloroplast localized plastoquinone oxygen oxidoreductase suggested to have the potential to act as a photoprotective safety valve for photosynthesis. However, PTOX overexpression in plants has been unsuccessful at inducing photoprotection, and the factors that control its activity remain elusive. Here, we show that significant PTOX activity is induced in response to high light in the model species Eutrema salsugineum and Arabidopsis thaliana. This activation correlates with structural reorganization of the thylakoid membrane. Over-expression of PTOX in mutants of Arabidopsis thaliana perturbed in thylakoid stacking also results in such activity, in contrast to wild type plants with normal granal structure. Further, PTOX activation protects against photoinhibition of Photosystem II and reduces reactive oxygen production under stress conditions. We conclude that structural re-arrangements of the thylakoid membranes, bringing Photosystem II and PTOX into proximity, are both required and sufficient for PTOX to act as a Photosystem II sink and play a role in photoprotection.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/264573
Calzadilla, Pablo Ignacio; Song, Junliang; Gallois, Patrick; Johnson, Giles Nicholas; Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection; Springer; Nature Communications; 15; 1; 1-2024; 1-13
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/264573
identifier_str_mv Calzadilla, Pablo Ignacio; Song, Junliang; Gallois, Patrick; Johnson, Giles Nicholas; Proximity to Photosystem II is necessary for activation of Plastid Terminal Oxidase (PTOX) for photoprotection; Springer; Nature Communications; 15; 1; 1-2024; 1-13
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-023-44454-x
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-023-44454-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.065482

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