Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii

Autores
Gonorazky, Ana Gabriela; Oresti, Gerardo Martin; Carro, María de las Mercedes; Beligni, María Verónica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. A key aspect is finding conditions that trigger TAG production without reducing cell division. We identified a soluble diacylglycerol acyltransferase (DGAT), exclusive to the green lineage and moderately related to plant DGAT3, with heterologous DGAT activity. We demonstrated that DGAT3 localizes to the chloroplast in the model green alga Chlamydomonas reinhardtii and that its expression is induced by light in the presence of acetate, consistent with TAG accumulation. The light-dependent expression of DGAT3 and the presence of a 2Fe-2S iron-sulfur cluster-binding domain in its sequence indicates that this protein could accept electrons, directly or indirectly, from the photosynthetic machinery. The aim of this study was to investigate the relationship between DGAT3 expression and photosynthetic electron transport. With that purpose, we incubated C. reinhardtii wild type cc-125 cells with two photosystem II (PSII) inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone (DBMIB), and one photosystem I (PSI) inhibitor, N,N′-dimethyl-4 4′-bipyridinium dichloride (paraquat). Both, Dgat3 mRNAs and TAGs increased in DCMU and DBMIB-treated cells as early as 15 minutes after initiating the experiment, whereas no significant variations were observed in paraquat-treated cells in the course of a 3 h incubation period. Our results suggest that DGAT3 expression and TAG biosynthesis increase when PSII is over-reduced in order to avoid photodamage. This occurs naturally in situations in which light absorption is higher than the rate of photosynthesis (e.g. during illumination at high light intensity) or artificially in chemically-altered PSII centers. Currently, this hypothesis is being evaluated in mutants that have deficiencies in the function of PSI, PSII or the cytb6f complex.
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Carro, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Beligni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
DIACYLGLYCEROL ACYLTRANSFERASE
PHOTOPROTECTION
PHOTOSYNTHESIS
TRIACYLGLYCEROLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236430
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtiiGonorazky, Ana GabrielaOresti, Gerardo MartinCarro, María de las MercedesBeligni, María VerónicaDIACYLGLYCEROL ACYLTRANSFERASEPHOTOPROTECTIONPHOTOSYNTHESISTRIACYLGLYCEROLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. A key aspect is finding conditions that trigger TAG production without reducing cell division. We identified a soluble diacylglycerol acyltransferase (DGAT), exclusive to the green lineage and moderately related to plant DGAT3, with heterologous DGAT activity. We demonstrated that DGAT3 localizes to the chloroplast in the model green alga Chlamydomonas reinhardtii and that its expression is induced by light in the presence of acetate, consistent with TAG accumulation. The light-dependent expression of DGAT3 and the presence of a 2Fe-2S iron-sulfur cluster-binding domain in its sequence indicates that this protein could accept electrons, directly or indirectly, from the photosynthetic machinery. The aim of this study was to investigate the relationship between DGAT3 expression and photosynthetic electron transport. With that purpose, we incubated C. reinhardtii wild type cc-125 cells with two photosystem II (PSII) inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone (DBMIB), and one photosystem I (PSI) inhibitor, N,N′-dimethyl-4 4′-bipyridinium dichloride (paraquat). Both, Dgat3 mRNAs and TAGs increased in DCMU and DBMIB-treated cells as early as 15 minutes after initiating the experiment, whereas no significant variations were observed in paraquat-treated cells in the course of a 3 h incubation period. Our results suggest that DGAT3 expression and TAG biosynthesis increase when PSII is over-reduced in order to avoid photodamage. This occurs naturally in situations in which light absorption is higher than the rate of photosynthesis (e.g. during illumination at high light intensity) or artificially in chemically-altered PSII centers. Currently, this hypothesis is being evaluated in mutants that have deficiencies in the function of PSI, PSII or the cytb6f complex.Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Carro, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Beligni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaLVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchMendozaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236430Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-1770327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-47.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:52Zoai:ri.conicet.gov.ar:11336/236430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:53.044CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
title Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
spellingShingle Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
Gonorazky, Ana Gabriela
DIACYLGLYCEROL ACYLTRANSFERASE
PHOTOPROTECTION
PHOTOSYNTHESIS
TRIACYLGLYCEROLS
title_short Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
title_full Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
title_fullStr Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
title_full_unstemmed Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
title_sort Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Gonorazky, Ana Gabriela
Oresti, Gerardo Martin
Carro, María de las Mercedes
Beligni, María Verónica
author Gonorazky, Ana Gabriela
author_facet Gonorazky, Ana Gabriela
Oresti, Gerardo Martin
Carro, María de las Mercedes
Beligni, María Verónica
author_role author
author2 Oresti, Gerardo Martin
Carro, María de las Mercedes
Beligni, María Verónica
author2_role author
author
author
dc.subject.none.fl_str_mv DIACYLGLYCEROL ACYLTRANSFERASE
PHOTOPROTECTION
PHOTOSYNTHESIS
TRIACYLGLYCEROLS
topic DIACYLGLYCEROL ACYLTRANSFERASE
PHOTOPROTECTION
PHOTOSYNTHESIS
TRIACYLGLYCEROLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. A key aspect is finding conditions that trigger TAG production without reducing cell division. We identified a soluble diacylglycerol acyltransferase (DGAT), exclusive to the green lineage and moderately related to plant DGAT3, with heterologous DGAT activity. We demonstrated that DGAT3 localizes to the chloroplast in the model green alga Chlamydomonas reinhardtii and that its expression is induced by light in the presence of acetate, consistent with TAG accumulation. The light-dependent expression of DGAT3 and the presence of a 2Fe-2S iron-sulfur cluster-binding domain in its sequence indicates that this protein could accept electrons, directly or indirectly, from the photosynthetic machinery. The aim of this study was to investigate the relationship between DGAT3 expression and photosynthetic electron transport. With that purpose, we incubated C. reinhardtii wild type cc-125 cells with two photosystem II (PSII) inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone (DBMIB), and one photosystem I (PSI) inhibitor, N,N′-dimethyl-4 4′-bipyridinium dichloride (paraquat). Both, Dgat3 mRNAs and TAGs increased in DCMU and DBMIB-treated cells as early as 15 minutes after initiating the experiment, whereas no significant variations were observed in paraquat-treated cells in the course of a 3 h incubation period. Our results suggest that DGAT3 expression and TAG biosynthesis increase when PSII is over-reduced in order to avoid photodamage. This occurs naturally in situations in which light absorption is higher than the rate of photosynthesis (e.g. during illumination at high light intensity) or artificially in chemically-altered PSII centers. Currently, this hypothesis is being evaluated in mutants that have deficiencies in the function of PSI, PSII or the cytb6f complex.
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Carro, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Beligni, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. A key aspect is finding conditions that trigger TAG production without reducing cell division. We identified a soluble diacylglycerol acyltransferase (DGAT), exclusive to the green lineage and moderately related to plant DGAT3, with heterologous DGAT activity. We demonstrated that DGAT3 localizes to the chloroplast in the model green alga Chlamydomonas reinhardtii and that its expression is induced by light in the presence of acetate, consistent with TAG accumulation. The light-dependent expression of DGAT3 and the presence of a 2Fe-2S iron-sulfur cluster-binding domain in its sequence indicates that this protein could accept electrons, directly or indirectly, from the photosynthetic machinery. The aim of this study was to investigate the relationship between DGAT3 expression and photosynthetic electron transport. With that purpose, we incubated C. reinhardtii wild type cc-125 cells with two photosystem II (PSII) inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone (DBMIB), and one photosystem I (PSI) inhibitor, N,N′-dimethyl-4 4′-bipyridinium dichloride (paraquat). Both, Dgat3 mRNAs and TAGs increased in DCMU and DBMIB-treated cells as early as 15 minutes after initiating the experiment, whereas no significant variations were observed in paraquat-treated cells in the course of a 3 h incubation period. Our results suggest that DGAT3 expression and TAG biosynthesis increase when PSII is over-reduced in order to avoid photodamage. This occurs naturally in situations in which light absorption is higher than the rate of photosynthesis (e.g. during illumination at high light intensity) or artificially in chemically-altered PSII centers. Currently, this hypothesis is being evaluated in mutants that have deficiencies in the function of PSI, PSII or the cytb6f complex.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236430
Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-177
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236430
identifier_str_mv Inhibitors of photosystem II increase the expression of chloroplast diacylglycerol acyltransferase-3 and promote triacylglycerol accumulation in Chlamydomonas reinhardtii; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 176-177
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-47.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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