Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans

Autores
Henriquez, Maria A.; Wolski, Erika Alejandra; Molina, Oscar I.; Adam, Lorne R.; Andreu, Adriana Balbina; Daayf, Fouad
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of Phytophthora infestans glucans, eicosapentaenoic acid (EPA) and isolates of this pathogen, on the differential expression of eight genes from the phenylpropanoid and the mevalonate (Ac-MVA) pathways were analyzed in potato by semi-quantitative RT-PCR and qRT-PCR. The application of EPA had an elicitor effect in Russet Burbank (RB) and Defender (DF) in response to inoculation with a US8 isolate of P. infestans, thereby reducing symptoms of late blight. Such effect was associated with the expression of PAL-1 and PAL-2, since the latter occurred only when EPA was followed by inoculation, whereas these genes were down-regulated in individual treatments RB + EPA, RB + US8, DF + EPA, and DF + US8. The glucan fraction did not by itself suppress phenylpropanoid genes, but its combination with the pathogen resulted in a down-regulation of PAL-1, PAL-2 and CHS. The addition of the glucan fraction to the elicitor EPA, had a negative effect (RB + EPA + GL + US8) since plants showed higher disease symptoms than the ones pretreated with water then infected with US8, and in comparison with RB + EPA + US8 and RB + GL + US8. Exclusive up-regulation of 4CL in DF + US11 and of CHS in DF + EPA + GL + US8, DF + EPA + US11, DF + GL + US11 and DF + EPA + GL + US11, where late blight lesions were not detected, could be associated with potato protection against late blight. Along with previous findings in this pathosystem, these data suggest that genetic resistance in potato against P. infestans is not the result of isolated reactions against the pathogen, but rather the combination of many factors in-line with a polygenic/horizontal resistance.
Fil: Henriquez, Maria A.. University Of Manitoba; Canadá
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Oscar I.. University Of Manitoba; Canadá
Fil: Adam, Lorne R.. University Of Manitoba; Canadá
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Daayf, Fouad. University Of Manitoba; Canadá
Materia
Oomycetes
Effectors
Phenolics
Phenylpropanoid
Defense Inducers
Defense Suppressors
Late Blight
Terpenoids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13061
Acceder
id CONICETDig_4ff5a0c515053eb3de52d69207daa8e1
oai_identifier_str oai:ri.conicet.gov.ar:11336/13061
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestansHenriquez, Maria A.Wolski, Erika AlejandraMolina, Oscar I.Adam, Lorne R.Andreu, Adriana BalbinaDaayf, FouadOomycetesEffectorsPhenolicsPhenylpropanoidDefense InducersDefense SuppressorsLate BlightTerpenoidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of Phytophthora infestans glucans, eicosapentaenoic acid (EPA) and isolates of this pathogen, on the differential expression of eight genes from the phenylpropanoid and the mevalonate (Ac-MVA) pathways were analyzed in potato by semi-quantitative RT-PCR and qRT-PCR. The application of EPA had an elicitor effect in Russet Burbank (RB) and Defender (DF) in response to inoculation with a US8 isolate of P. infestans, thereby reducing symptoms of late blight. Such effect was associated with the expression of PAL-1 and PAL-2, since the latter occurred only when EPA was followed by inoculation, whereas these genes were down-regulated in individual treatments RB + EPA, RB + US8, DF + EPA, and DF + US8. The glucan fraction did not by itself suppress phenylpropanoid genes, but its combination with the pathogen resulted in a down-regulation of PAL-1, PAL-2 and CHS. The addition of the glucan fraction to the elicitor EPA, had a negative effect (RB + EPA + GL + US8) since plants showed higher disease symptoms than the ones pretreated with water then infected with US8, and in comparison with RB + EPA + US8 and RB + GL + US8. Exclusive up-regulation of 4CL in DF + US11 and of CHS in DF + EPA + GL + US8, DF + EPA + US11, DF + GL + US11 and DF + EPA + GL + US11, where late blight lesions were not detected, could be associated with potato protection against late blight. Along with previous findings in this pathosystem, these data suggest that genetic resistance in potato against P. infestans is not the result of isolated reactions against the pathogen, but rather the combination of many factors in-line with a polygenic/horizontal resistance.Fil: Henriquez, Maria A.. University Of Manitoba; CanadáFil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molina, Oscar I.. University Of Manitoba; CanadáFil: Adam, Lorne R.. University Of Manitoba; CanadáFil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Daayf, Fouad. University Of Manitoba; CanadáElsevier Masson2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13061Henriquez, Maria A.; Wolski, Erika Alejandra; Molina, Oscar I.; Adam, Lorne R.; Andreu, Adriana Balbina; et al.; Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans; Elsevier Masson; Plant Physiology And Biochemistry; 60; 11-2012; 119-1280981-9428enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942812002069info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2012.07.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:25Zoai:ri.conicet.gov.ar:11336/13061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:26.062CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
title Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
spellingShingle Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
Henriquez, Maria A.
Oomycetes
Effectors
Phenolics
Phenylpropanoid
Defense Inducers
Defense Suppressors
Late Blight
Terpenoids
title_short Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
title_full Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
title_fullStr Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
title_full_unstemmed Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
title_sort Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans
dc.creator.none.fl_str_mv Henriquez, Maria A.
Wolski, Erika Alejandra
Molina, Oscar I.
Adam, Lorne R.
Andreu, Adriana Balbina
Daayf, Fouad
author Henriquez, Maria A.
author_facet Henriquez, Maria A.
Wolski, Erika Alejandra
Molina, Oscar I.
Adam, Lorne R.
Andreu, Adriana Balbina
Daayf, Fouad
author_role author
author2 Wolski, Erika Alejandra
Molina, Oscar I.
Adam, Lorne R.
Andreu, Adriana Balbina
Daayf, Fouad
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Oomycetes
Effectors
Phenolics
Phenylpropanoid
Defense Inducers
Defense Suppressors
Late Blight
Terpenoids
topic Oomycetes
Effectors
Phenolics
Phenylpropanoid
Defense Inducers
Defense Suppressors
Late Blight
Terpenoids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effects of Phytophthora infestans glucans, eicosapentaenoic acid (EPA) and isolates of this pathogen, on the differential expression of eight genes from the phenylpropanoid and the mevalonate (Ac-MVA) pathways were analyzed in potato by semi-quantitative RT-PCR and qRT-PCR. The application of EPA had an elicitor effect in Russet Burbank (RB) and Defender (DF) in response to inoculation with a US8 isolate of P. infestans, thereby reducing symptoms of late blight. Such effect was associated with the expression of PAL-1 and PAL-2, since the latter occurred only when EPA was followed by inoculation, whereas these genes were down-regulated in individual treatments RB + EPA, RB + US8, DF + EPA, and DF + US8. The glucan fraction did not by itself suppress phenylpropanoid genes, but its combination with the pathogen resulted in a down-regulation of PAL-1, PAL-2 and CHS. The addition of the glucan fraction to the elicitor EPA, had a negative effect (RB + EPA + GL + US8) since plants showed higher disease symptoms than the ones pretreated with water then infected with US8, and in comparison with RB + EPA + US8 and RB + GL + US8. Exclusive up-regulation of 4CL in DF + US11 and of CHS in DF + EPA + GL + US8, DF + EPA + US11, DF + GL + US11 and DF + EPA + GL + US11, where late blight lesions were not detected, could be associated with potato protection against late blight. Along with previous findings in this pathosystem, these data suggest that genetic resistance in potato against P. infestans is not the result of isolated reactions against the pathogen, but rather the combination of many factors in-line with a polygenic/horizontal resistance.
Fil: Henriquez, Maria A.. University Of Manitoba; Canadá
Fil: Wolski, Erika Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molina, Oscar I.. University Of Manitoba; Canadá
Fil: Adam, Lorne R.. University Of Manitoba; Canadá
Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Daayf, Fouad. University Of Manitoba; Canadá
description The effects of Phytophthora infestans glucans, eicosapentaenoic acid (EPA) and isolates of this pathogen, on the differential expression of eight genes from the phenylpropanoid and the mevalonate (Ac-MVA) pathways were analyzed in potato by semi-quantitative RT-PCR and qRT-PCR. The application of EPA had an elicitor effect in Russet Burbank (RB) and Defender (DF) in response to inoculation with a US8 isolate of P. infestans, thereby reducing symptoms of late blight. Such effect was associated with the expression of PAL-1 and PAL-2, since the latter occurred only when EPA was followed by inoculation, whereas these genes were down-regulated in individual treatments RB + EPA, RB + US8, DF + EPA, and DF + US8. The glucan fraction did not by itself suppress phenylpropanoid genes, but its combination with the pathogen resulted in a down-regulation of PAL-1, PAL-2 and CHS. The addition of the glucan fraction to the elicitor EPA, had a negative effect (RB + EPA + GL + US8) since plants showed higher disease symptoms than the ones pretreated with water then infected with US8, and in comparison with RB + EPA + US8 and RB + GL + US8. Exclusive up-regulation of 4CL in DF + US11 and of CHS in DF + EPA + GL + US8, DF + EPA + US11, DF + GL + US11 and DF + EPA + GL + US11, where late blight lesions were not detected, could be associated with potato protection against late blight. Along with previous findings in this pathosystem, these data suggest that genetic resistance in potato against P. infestans is not the result of isolated reactions against the pathogen, but rather the combination of many factors in-line with a polygenic/horizontal resistance.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13061
Henriquez, Maria A.; Wolski, Erika Alejandra; Molina, Oscar I.; Adam, Lorne R.; Andreu, Adriana Balbina; et al.; Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans; Elsevier Masson; Plant Physiology And Biochemistry; 60; 11-2012; 119-128
0981-9428
url http://hdl.handle.net/11336/13061
identifier_str_mv Henriquez, Maria A.; Wolski, Erika Alejandra; Molina, Oscar I.; Adam, Lorne R.; Andreu, Adriana Balbina; et al.; Effects of glucan and eicosapetaeonoic acids on differential regulation on phenilpropanoids and mevalonic pathways during potato response to Phytophthora infestans; Elsevier Masson; Plant Physiology And Biochemistry; 60; 11-2012; 119-128
0981-9428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942812002069
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2012.07.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson
publisher.none.fl_str_mv Elsevier Masson
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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