Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes

Autores
Marziali, Federico Emanuel; Cavatorta, Ana Laura; Bugnon Valdano, Marina Paula; Facciuto, Florencia Natalia; Gardiol, Daniela Nora
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously reported the identification of an alternative splicing event in the 5 ′ untranslated region (5 ′ -UTR) of DLG1 mRNA that generates transcripts with two different 5 ′ -UTR (short and large 5 ′ -UTR variants). In this study, we further examined the impact of the DLG1 transcription and the role of the differential expression of the alternative 5 ′ -UTRs on DLG1 protein levels. We analysed these mechanisms during cell processes like differentiation, cell cycle progression and cell-cell contact formation, where the importance of DLG1 activities was previously established. The data presented in this report suggest that the transcriptional regulation of DLG1 strongly contributes to DLG1 abundance and that differential expression of alternative 5 ′ -UTRs with different translational properties, also cooperates, depending on the cell type and cell situation. This study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes.
Fil: Marziali, Federico Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cavatorta, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bugnon Valdano, Marina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Facciuto, Florencia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gardiol, Daniela Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Dlg1
Expression
Polarity
Transcription Regulation
5' Utr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21474
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21474
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processesMarziali, Federico EmanuelCavatorta, Ana LauraBugnon Valdano, Marina PaulaFacciuto, Florencia NataliaGardiol, Daniela NoraDlg1ExpressionPolarityTranscription Regulation5' Utrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously reported the identification of an alternative splicing event in the 5 ′ untranslated region (5 ′ -UTR) of DLG1 mRNA that generates transcripts with two different 5 ′ -UTR (short and large 5 ′ -UTR variants). In this study, we further examined the impact of the DLG1 transcription and the role of the differential expression of the alternative 5 ′ -UTRs on DLG1 protein levels. We analysed these mechanisms during cell processes like differentiation, cell cycle progression and cell-cell contact formation, where the importance of DLG1 activities was previously established. The data presented in this report suggest that the transcriptional regulation of DLG1 strongly contributes to DLG1 abundance and that differential expression of alternative 5 ′ -UTRs with different translational properties, also cooperates, depending on the cell type and cell situation. This study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes.Fil: Marziali, Federico Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Cavatorta, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bugnon Valdano, Marina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Facciuto, Florencia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gardiol, Daniela Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentinade Gruyter2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21474Marziali, Federico Emanuel; Cavatorta, Ana Laura; Bugnon Valdano, Marina Paula; Facciuto, Florencia Natalia; Gardiol, Daniela Nora; Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes; de Gruyter; Biological Chemistry; 396; 8; 3-2015; 1-101431-67301437-4315CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/hsz-2014-0286info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/bchm.2015.396.issue-8/hsz-2014-0286/hsz-2014-0286.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:30Zoai:ri.conicet.gov.ar:11336/21474instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:30.385CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
title Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
spellingShingle Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
Marziali, Federico Emanuel
Dlg1
Expression
Polarity
Transcription Regulation
5' Utr
title_short Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
title_full Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
title_fullStr Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
title_full_unstemmed Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
title_sort Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes
dc.creator.none.fl_str_mv Marziali, Federico Emanuel
Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela Nora
author Marziali, Federico Emanuel
author_facet Marziali, Federico Emanuel
Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela Nora
author_role author
author2 Cavatorta, Ana Laura
Bugnon Valdano, Marina Paula
Facciuto, Florencia Natalia
Gardiol, Daniela Nora
author2_role author
author
author
author
dc.subject.none.fl_str_mv Dlg1
Expression
Polarity
Transcription Regulation
5' Utr
topic Dlg1
Expression
Polarity
Transcription Regulation
5' Utr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously reported the identification of an alternative splicing event in the 5 ′ untranslated region (5 ′ -UTR) of DLG1 mRNA that generates transcripts with two different 5 ′ -UTR (short and large 5 ′ -UTR variants). In this study, we further examined the impact of the DLG1 transcription and the role of the differential expression of the alternative 5 ′ -UTRs on DLG1 protein levels. We analysed these mechanisms during cell processes like differentiation, cell cycle progression and cell-cell contact formation, where the importance of DLG1 activities was previously established. The data presented in this report suggest that the transcriptional regulation of DLG1 strongly contributes to DLG1 abundance and that differential expression of alternative 5 ′ -UTRs with different translational properties, also cooperates, depending on the cell type and cell situation. This study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes.
Fil: Marziali, Federico Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cavatorta, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bugnon Valdano, Marina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Facciuto, Florencia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gardiol, Daniela Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Human discs large (DLG1) has been demonstrated to be involved in cell polarity and maintenance of tissue architecture. However, the mechanisms controlling DLG1 expression are not fully understood. This is relevant as DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously reported the identification of an alternative splicing event in the 5 ′ untranslated region (5 ′ -UTR) of DLG1 mRNA that generates transcripts with two different 5 ′ -UTR (short and large 5 ′ -UTR variants). In this study, we further examined the impact of the DLG1 transcription and the role of the differential expression of the alternative 5 ′ -UTRs on DLG1 protein levels. We analysed these mechanisms during cell processes like differentiation, cell cycle progression and cell-cell contact formation, where the importance of DLG1 activities was previously established. The data presented in this report suggest that the transcriptional regulation of DLG1 strongly contributes to DLG1 abundance and that differential expression of alternative 5 ′ -UTRs with different translational properties, also cooperates, depending on the cell type and cell situation. This study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21474
Marziali, Federico Emanuel; Cavatorta, Ana Laura; Bugnon Valdano, Marina Paula; Facciuto, Florencia Natalia; Gardiol, Daniela Nora; Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes; de Gruyter; Biological Chemistry; 396; 8; 3-2015; 1-10
1431-6730
1437-4315
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21474
identifier_str_mv Marziali, Federico Emanuel; Cavatorta, Ana Laura; Bugnon Valdano, Marina Paula; Facciuto, Florencia Natalia; Gardiol, Daniela Nora; Transcriptional and translational mechanisms contribute to regulate the expression of Discs Large 1 protein during different biological processes; de Gruyter; Biological Chemistry; 396; 8; 3-2015; 1-10
1431-6730
1437-4315
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/hsz-2014-0286
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/bchm.2015.396.issue-8/hsz-2014-0286/hsz-2014-0286.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv de Gruyter
publisher.none.fl_str_mv de Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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