Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region

Autores
Ferrero, Marcela Alejandra; Menoyo, Eugenia; Lugo, Mónica Alejandra; Negritto, María de Los Angeles; Farias, Maria Eugenia; Anton, Ana Maria Ramona; Siñeriz, Faustino
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Puna is a harsh bio-geographical region in South America that characterized by distinct plant diversity, high aridity and irradiance. In the present study, rhizospheric bacterial communities were analyzed along an altitudinal gradient in this region while considering the different photosynthesis pathways (C3/C4) of the native grasses. This analysis included cultivation-dependent and -independent approaches such as PCR-DGGE analysis of the 16S rRNA genes and FISH. DGGE revealed that the band richness differed along the altitudinal gradient, but that it did not differ significantly among plants with different metabolic pathways. Overall, we found that Bacillus and Pseudomonas were the dominant phylotypes based on the DGGE analysis. Additionally, nearly 80% of the species identified by PCR were also identified using the cultivation method. FISH analysis revealed that the Gammaproteobacteria and Actinobacteria were the dominant bacterial groups at most sites, followed by Archaea. Finally, low bacterial diversity was detected in samples collected from all heights, possibly due to the harsh environment. Overall, evaluation of the results obtained using the two different approaches revealed that the culture method is efficient for screening of the bacterial community in Puna rhizospheric soils. Additionally, we discuss possible effects of Puna rhizospheric bacteria on the protection of native grasses and nutrient capture.
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lugo, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Negritto, María de Los Angeles. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanográficas. Departamento de Botánica; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Anton, Ana Maria Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Andean Highlands
Dgge
Fish
Mountain Native Poaceae
Rhizosphere Bacteria Community
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72693
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72693
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna regionFerrero, Marcela AlejandraMenoyo, EugeniaLugo, Mónica AlejandraNegritto, María de Los AngelesFarias, Maria EugeniaAnton, Ana Maria RamonaSiñeriz, FaustinoAndean HighlandsDggeFishMountain Native PoaceaeRhizosphere Bacteria Communityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Puna is a harsh bio-geographical region in South America that characterized by distinct plant diversity, high aridity and irradiance. In the present study, rhizospheric bacterial communities were analyzed along an altitudinal gradient in this region while considering the different photosynthesis pathways (C3/C4) of the native grasses. This analysis included cultivation-dependent and -independent approaches such as PCR-DGGE analysis of the 16S rRNA genes and FISH. DGGE revealed that the band richness differed along the altitudinal gradient, but that it did not differ significantly among plants with different metabolic pathways. Overall, we found that Bacillus and Pseudomonas were the dominant phylotypes based on the DGGE analysis. Additionally, nearly 80% of the species identified by PCR were also identified using the cultivation method. FISH analysis revealed that the Gammaproteobacteria and Actinobacteria were the dominant bacterial groups at most sites, followed by Archaea. Finally, low bacterial diversity was detected in samples collected from all heights, possibly due to the harsh environment. Overall, evaluation of the results obtained using the two different approaches revealed that the culture method is efficient for screening of the bacterial community in Puna rhizospheric soils. Additionally, we discuss possible effects of Puna rhizospheric bacteria on the protection of native grasses and nutrient capture.Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Lugo, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Negritto, María de Los Angeles. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanográficas. Departamento de Botánica; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Anton, Ana Maria Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72693Ferrero, Marcela Alejandra; Menoyo, Eugenia; Lugo, Mónica Alejandra; Negritto, María de Los Angeles; Farias, Maria Eugenia; et al.; Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 74; 10; 10-2010; 1177-11850140-1963CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2010.04.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0140196310001060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:03Zoai:ri.conicet.gov.ar:11336/72693instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:03.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
title Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
spellingShingle Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
Ferrero, Marcela Alejandra
Andean Highlands
Dgge
Fish
Mountain Native Poaceae
Rhizosphere Bacteria Community
title_short Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
title_full Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
title_fullStr Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
title_full_unstemmed Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
title_sort Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region
dc.creator.none.fl_str_mv Ferrero, Marcela Alejandra
Menoyo, Eugenia
Lugo, Mónica Alejandra
Negritto, María de Los Angeles
Farias, Maria Eugenia
Anton, Ana Maria Ramona
Siñeriz, Faustino
author Ferrero, Marcela Alejandra
author_facet Ferrero, Marcela Alejandra
Menoyo, Eugenia
Lugo, Mónica Alejandra
Negritto, María de Los Angeles
Farias, Maria Eugenia
Anton, Ana Maria Ramona
Siñeriz, Faustino
author_role author
author2 Menoyo, Eugenia
Lugo, Mónica Alejandra
Negritto, María de Los Angeles
Farias, Maria Eugenia
Anton, Ana Maria Ramona
Siñeriz, Faustino
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Andean Highlands
Dgge
Fish
Mountain Native Poaceae
Rhizosphere Bacteria Community
topic Andean Highlands
Dgge
Fish
Mountain Native Poaceae
Rhizosphere Bacteria Community
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Puna is a harsh bio-geographical region in South America that characterized by distinct plant diversity, high aridity and irradiance. In the present study, rhizospheric bacterial communities were analyzed along an altitudinal gradient in this region while considering the different photosynthesis pathways (C3/C4) of the native grasses. This analysis included cultivation-dependent and -independent approaches such as PCR-DGGE analysis of the 16S rRNA genes and FISH. DGGE revealed that the band richness differed along the altitudinal gradient, but that it did not differ significantly among plants with different metabolic pathways. Overall, we found that Bacillus and Pseudomonas were the dominant phylotypes based on the DGGE analysis. Additionally, nearly 80% of the species identified by PCR were also identified using the cultivation method. FISH analysis revealed that the Gammaproteobacteria and Actinobacteria were the dominant bacterial groups at most sites, followed by Archaea. Finally, low bacterial diversity was detected in samples collected from all heights, possibly due to the harsh environment. Overall, evaluation of the results obtained using the two different approaches revealed that the culture method is efficient for screening of the bacterial community in Puna rhizospheric soils. Additionally, we discuss possible effects of Puna rhizospheric bacteria on the protection of native grasses and nutrient capture.
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lugo, Mónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Negritto, María de Los Angeles. Universidad de Concepción. Facultad de Ciencias Naturales y Oceanográficas. Departamento de Botánica; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Anton, Ana Maria Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Puna is a harsh bio-geographical region in South America that characterized by distinct plant diversity, high aridity and irradiance. In the present study, rhizospheric bacterial communities were analyzed along an altitudinal gradient in this region while considering the different photosynthesis pathways (C3/C4) of the native grasses. This analysis included cultivation-dependent and -independent approaches such as PCR-DGGE analysis of the 16S rRNA genes and FISH. DGGE revealed that the band richness differed along the altitudinal gradient, but that it did not differ significantly among plants with different metabolic pathways. Overall, we found that Bacillus and Pseudomonas were the dominant phylotypes based on the DGGE analysis. Additionally, nearly 80% of the species identified by PCR were also identified using the cultivation method. FISH analysis revealed that the Gammaproteobacteria and Actinobacteria were the dominant bacterial groups at most sites, followed by Archaea. Finally, low bacterial diversity was detected in samples collected from all heights, possibly due to the harsh environment. Overall, evaluation of the results obtained using the two different approaches revealed that the culture method is efficient for screening of the bacterial community in Puna rhizospheric soils. Additionally, we discuss possible effects of Puna rhizospheric bacteria on the protection of native grasses and nutrient capture.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72693
Ferrero, Marcela Alejandra; Menoyo, Eugenia; Lugo, Mónica Alejandra; Negritto, María de Los Angeles; Farias, Maria Eugenia; et al.; Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 74; 10; 10-2010; 1177-1185
0140-1963
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72693
identifier_str_mv Ferrero, Marcela Alejandra; Menoyo, Eugenia; Lugo, Mónica Alejandra; Negritto, María de Los Angeles; Farias, Maria Eugenia; et al.; Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region; Academic Press Ltd - Elsevier Science Ltd; Journal of Arid Environments; 74; 10; 10-2010; 1177-1185
0140-1963
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2010.04.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0140196310001060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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