Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256

Autores
Giner Lamia, Joaquín; Vinuesa, Pablo; Betancor, Laura; Silva, Claudia; Bisio, Julieta; Soleto, Lorena; Chabalgoity, José A.; Puente, José Luis; Soncini, Fernando Carlos; Garcia Vescovi, Eleonora; García Del Portillo, Francisco
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport related-genes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host.
Fil: Giner Lamia, Joaquín. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Madrid; España
Fil: Vinuesa, Pablo. Universidad Nacional Autónoma de México; México
Fil: Betancor, Laura. Universidad de la República; Uruguay
Fil: Silva, Claudia. Universidad Nacional Autónoma de México; México
Fil: Bisio, Julieta. Universidad Nacional Autónoma de México; México
Fil: Soleto, Lorena. Universidad Autónoma Gabriel René Moreno; Bolivia. Ministerio de Salud de Bolivia. Centro Nacional de Enfermedades Tropicales; Bolivia
Fil: Chabalgoity, José A.. Universidad de la República; Uruguay
Fil: Puente, José Luis. Universidad Nacional Autónoma de México; México
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: García Del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España
Materia
COMPARATIVE GENOMICS
INVASIVE HUMAN INFECTIONS
SALMONELLA ENTERICA
SUBSPECIES DIARIZONAE
TYPE-III EFFECTORS
VIRULENCE GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151306
Acceder
id CONICETDig_4ea2f72579eef3f9850331bb4c2acde3
oai_identifier_str oai:ri.conicet.gov.ar:11336/151306
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256Giner Lamia, JoaquínVinuesa, PabloBetancor, LauraSilva, ClaudiaBisio, JulietaSoleto, LorenaChabalgoity, José A.Puente, José LuisSoncini, Fernando CarlosGarcia Vescovi, EleonoraGarcía Del Portillo, FranciscoCOMPARATIVE GENOMICSINVASIVE HUMAN INFECTIONSSALMONELLA ENTERICASUBSPECIES DIARIZONAETYPE-III EFFECTORSVIRULENCE GENEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport related-genes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host.Fil: Giner Lamia, Joaquín. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Madrid; EspañaFil: Vinuesa, Pablo. Universidad Nacional Autónoma de México; MéxicoFil: Betancor, Laura. Universidad de la República; UruguayFil: Silva, Claudia. Universidad Nacional Autónoma de México; MéxicoFil: Bisio, Julieta. Universidad Nacional Autónoma de México; MéxicoFil: Soleto, Lorena. Universidad Autónoma Gabriel René Moreno; Bolivia. Ministerio de Salud de Bolivia. Centro Nacional de Enfermedades Tropicales; BoliviaFil: Chabalgoity, José A.. Universidad de la República; UruguayFil: Puente, José Luis. Universidad Nacional Autónoma de México; MéxicoFil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: García Del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; EspañaBioMed Central2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151306Giner Lamia, Joaquín; Vinuesa, Pablo; Betancor, Laura; Silva, Claudia; Bisio, Julieta; et al.; Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256; BioMed Central; BMC Genomics; 20; 1; 1-2019; 1-141471-2164CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-5352-zinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-018-5352-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:31Zoai:ri.conicet.gov.ar:11336/151306instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:31.982CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
title Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
spellingShingle Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
Giner Lamia, Joaquín
COMPARATIVE GENOMICS
INVASIVE HUMAN INFECTIONS
SALMONELLA ENTERICA
SUBSPECIES DIARIZONAE
TYPE-III EFFECTORS
VIRULENCE GENES
title_short Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
title_full Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
title_fullStr Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
title_full_unstemmed Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
title_sort Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256
dc.creator.none.fl_str_mv Giner Lamia, Joaquín
Vinuesa, Pablo
Betancor, Laura
Silva, Claudia
Bisio, Julieta
Soleto, Lorena
Chabalgoity, José A.
Puente, José Luis
Soncini, Fernando Carlos
Garcia Vescovi, Eleonora
García Del Portillo, Francisco
author Giner Lamia, Joaquín
author_facet Giner Lamia, Joaquín
Vinuesa, Pablo
Betancor, Laura
Silva, Claudia
Bisio, Julieta
Soleto, Lorena
Chabalgoity, José A.
Puente, José Luis
Soncini, Fernando Carlos
Garcia Vescovi, Eleonora
García Del Portillo, Francisco
author_role author
author2 Vinuesa, Pablo
Betancor, Laura
Silva, Claudia
Bisio, Julieta
Soleto, Lorena
Chabalgoity, José A.
Puente, José Luis
Soncini, Fernando Carlos
Garcia Vescovi, Eleonora
García Del Portillo, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPARATIVE GENOMICS
INVASIVE HUMAN INFECTIONS
SALMONELLA ENTERICA
SUBSPECIES DIARIZONAE
TYPE-III EFFECTORS
VIRULENCE GENES
topic COMPARATIVE GENOMICS
INVASIVE HUMAN INFECTIONS
SALMONELLA ENTERICA
SUBSPECIES DIARIZONAE
TYPE-III EFFECTORS
VIRULENCE GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport related-genes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host.
Fil: Giner Lamia, Joaquín. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Madrid; España
Fil: Vinuesa, Pablo. Universidad Nacional Autónoma de México; México
Fil: Betancor, Laura. Universidad de la República; Uruguay
Fil: Silva, Claudia. Universidad Nacional Autónoma de México; México
Fil: Bisio, Julieta. Universidad Nacional Autónoma de México; México
Fil: Soleto, Lorena. Universidad Autónoma Gabriel René Moreno; Bolivia. Ministerio de Salud de Bolivia. Centro Nacional de Enfermedades Tropicales; Bolivia
Fil: Chabalgoity, José A.. Universidad de la República; Uruguay
Fil: Puente, José Luis. Universidad Nacional Autónoma de México; México
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: García Del Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España
description Background Salmonella enterica subsp. diarizonae (IIIb) is frequently isolated from the environment, cold-blooded reptiles, sheep and humans; however only a few studies describe the isolation of this subspecies from invasive human infections. The factors contributing to this unusual behavior are currently unknown. Results We report here the genome features of two diarizonae strains, SBO13 and SBO27, isolated from endocervical tissue collected post-abortion and from cerebrospinal fluid of a newborn child, respectively, in the city of Santa Cruz, Bolivia. Although isolated six years apart, SBO27 in 2008 and SBO13 in 2014, both strains belong to the same sequence type 1256 (ST1256) and show a high degree of genome conservation sharing more than 99% of their genes, including the conservation of a ~ 10 kb plasmid. A prominent feature of the two genomes is the presence of 24 genomic islands (GIs), in addition to 10 complete Salmonella pathogenicity islands (SPI) and fragments of SPI-7, a pathogenicity island first reported in the human-adapted serovar Typhi. Some of the GIs identified in SBO13 and SBO27 harbor genes putatively encoding auto-transporters involved in adhesion, lipopolysaccharide modifying enzymes, putative toxins, pili-related proteins, efflux pumps, and several putative membrane cation transport related-genes, among others. These two Bolivian isolates also share genes encoding the type-III secretion system effector proteins SseK2, SseK3 and SlrP with other diarizonae sequence types (ST) mainly-associated with infections in humans. The sseK2, sseK3 and slrP genes were either absent or showing frameshift mutations in a significant proportion of genomes from environmental diarizonae isolates. Conclusions The comparative genomic study of two diarizonae strains isolated in Bolivia from human patients uncovered the presence of many genes putatively related to virulence. The statistically-significant acquisition of a unique combination of these functions by diarizonae strains isolated from humans may have impacted the ability of these isolates to successfully infect the human host.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151306
Giner Lamia, Joaquín; Vinuesa, Pablo; Betancor, Laura; Silva, Claudia; Bisio, Julieta; et al.; Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256; BioMed Central; BMC Genomics; 20; 1; 1-2019; 1-14
1471-2164
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151306
identifier_str_mv Giner Lamia, Joaquín; Vinuesa, Pablo; Betancor, Laura; Silva, Claudia; Bisio, Julieta; et al.; Genome analysis of Salmonella enterica subsp. diarizonae isolates from invasive human infections reveals enrichment of virulence-related functions in lineage ST1256; BioMed Central; BMC Genomics; 20; 1; 1-2019; 1-14
1471-2164
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-018-5352-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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