Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California

Autores
Camiletti, Boris Xavier; Lichtemberg, Paulo dos Santos Faria; Paredes, Juan Andres; Carraro, Thiago A.; Velasco, Jhordan; Michailides, Themis J.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dieback caused by Colletotrichum spp. is an emerging disease in California citrus groves. A large-scale survey with emphasis on seasonal variations of latent infections was conducted throughout citrus orchards in Fresno, Kern, and Tulare counties in 2019 and 2020. Latent infections on citrus leaves and twigs varied markedly between years. Isolates of Colletotrichum spp. were obtained from asymptomatic tissue and two groups were formed based on colony and spore morphology. The morphological groups were further identified based on multigene sequence analysis using the DNA regions ITS1-5.8S-ITS2, TUB2, and GAPDH. Results revealed that isolates belong to two phylogenetic species, C. gloeosporioides and C. karstii, being C. karstii more frequently isolated. Representative isolates of each species were further selected and characterized based on the response of physiological variables to temperature. Both species had similar optimum growth temperatures but differed in maximum growth rates, with C. gloeosporioides exhibiting a greater growth rate than that of C. karstii on media. Pathogenicity tests on citrus trees demonstrated the ability of C. gloeosporioides and C. karstii to cause lesions on twigs and no differences in aggressiveness. A fungicide screening performed in this study determined that the DMI fungicides were the most effective in reducing the mycelial growth of C. gloeosporioides and C. karstii. The QoI fungicides showed a remarkably inhibitory impact on spore germination of both species. On average, C. karstii was more sensitive to the DMI fungicides than C. gloeosporioides. The findings of this study provide new information to understand the Colletotrichum dieback of citrus.
Fil: Camiletti, Boris Xavier. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lichtemberg, Paulo dos Santos Faria. University of California at Davis; Estados Unidos
Fil: Paredes, Juan Andres. University of California at Davis; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carraro, Thiago A.. University of California at Davis; Estados Unidos
Fil: Velasco, Jhordan. University of California at Davis; Estados Unidos
Fil: Michailides, Themis J.. University of California at Davis; Estados Unidos
Materia
MANDARIN
ANTHRACNOS
FUNGICIDE
EPIDEMIOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/160393
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/160393
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in CaliforniaCamiletti, Boris XavierLichtemberg, Paulo dos Santos FariaParedes, Juan AndresCarraro, Thiago A.Velasco, JhordanMichailides, Themis J.MANDARINANTHRACNOSFUNGICIDEEPIDEMIOLOGYhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Dieback caused by Colletotrichum spp. is an emerging disease in California citrus groves. A large-scale survey with emphasis on seasonal variations of latent infections was conducted throughout citrus orchards in Fresno, Kern, and Tulare counties in 2019 and 2020. Latent infections on citrus leaves and twigs varied markedly between years. Isolates of Colletotrichum spp. were obtained from asymptomatic tissue and two groups were formed based on colony and spore morphology. The morphological groups were further identified based on multigene sequence analysis using the DNA regions ITS1-5.8S-ITS2, TUB2, and GAPDH. Results revealed that isolates belong to two phylogenetic species, C. gloeosporioides and C. karstii, being C. karstii more frequently isolated. Representative isolates of each species were further selected and characterized based on the response of physiological variables to temperature. Both species had similar optimum growth temperatures but differed in maximum growth rates, with C. gloeosporioides exhibiting a greater growth rate than that of C. karstii on media. Pathogenicity tests on citrus trees demonstrated the ability of C. gloeosporioides and C. karstii to cause lesions on twigs and no differences in aggressiveness. A fungicide screening performed in this study determined that the DMI fungicides were the most effective in reducing the mycelial growth of C. gloeosporioides and C. karstii. The QoI fungicides showed a remarkably inhibitory impact on spore germination of both species. On average, C. karstii was more sensitive to the DMI fungicides than C. gloeosporioides. The findings of this study provide new information to understand the Colletotrichum dieback of citrus.Fil: Camiletti, Boris Xavier. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Lichtemberg, Paulo dos Santos Faria. University of California at Davis; Estados UnidosFil: Paredes, Juan Andres. University of California at Davis; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carraro, Thiago A.. University of California at Davis; Estados UnidosFil: Velasco, Jhordan. University of California at Davis; Estados UnidosFil: Michailides, Themis J.. University of California at Davis; Estados UnidosAmerican Phytopathological Society2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160393Camiletti, Boris Xavier; Lichtemberg, Paulo dos Santos Faria; Paredes, Juan Andres; Carraro, Thiago A.; Velasco, Jhordan; et al.; Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California; American Phytopathological Society; Phytopathology; 6-2022; 1-430031-949X1943-7684CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-21-0434-Rinfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-10-21-0434-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:34:03Zoai:ri.conicet.gov.ar:11336/160393instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:34:03.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
title Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
spellingShingle Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
Camiletti, Boris Xavier
MANDARIN
ANTHRACNOS
FUNGICIDE
EPIDEMIOLOGY
title_short Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
title_full Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
title_fullStr Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
title_full_unstemmed Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
title_sort Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California
dc.creator.none.fl_str_mv Camiletti, Boris Xavier
Lichtemberg, Paulo dos Santos Faria
Paredes, Juan Andres
Carraro, Thiago A.
Velasco, Jhordan
Michailides, Themis J.
author Camiletti, Boris Xavier
author_facet Camiletti, Boris Xavier
Lichtemberg, Paulo dos Santos Faria
Paredes, Juan Andres
Carraro, Thiago A.
Velasco, Jhordan
Michailides, Themis J.
author_role author
author2 Lichtemberg, Paulo dos Santos Faria
Paredes, Juan Andres
Carraro, Thiago A.
Velasco, Jhordan
Michailides, Themis J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MANDARIN
ANTHRACNOS
FUNGICIDE
EPIDEMIOLOGY
topic MANDARIN
ANTHRACNOS
FUNGICIDE
EPIDEMIOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Dieback caused by Colletotrichum spp. is an emerging disease in California citrus groves. A large-scale survey with emphasis on seasonal variations of latent infections was conducted throughout citrus orchards in Fresno, Kern, and Tulare counties in 2019 and 2020. Latent infections on citrus leaves and twigs varied markedly between years. Isolates of Colletotrichum spp. were obtained from asymptomatic tissue and two groups were formed based on colony and spore morphology. The morphological groups were further identified based on multigene sequence analysis using the DNA regions ITS1-5.8S-ITS2, TUB2, and GAPDH. Results revealed that isolates belong to two phylogenetic species, C. gloeosporioides and C. karstii, being C. karstii more frequently isolated. Representative isolates of each species were further selected and characterized based on the response of physiological variables to temperature. Both species had similar optimum growth temperatures but differed in maximum growth rates, with C. gloeosporioides exhibiting a greater growth rate than that of C. karstii on media. Pathogenicity tests on citrus trees demonstrated the ability of C. gloeosporioides and C. karstii to cause lesions on twigs and no differences in aggressiveness. A fungicide screening performed in this study determined that the DMI fungicides were the most effective in reducing the mycelial growth of C. gloeosporioides and C. karstii. The QoI fungicides showed a remarkably inhibitory impact on spore germination of both species. On average, C. karstii was more sensitive to the DMI fungicides than C. gloeosporioides. The findings of this study provide new information to understand the Colletotrichum dieback of citrus.
Fil: Camiletti, Boris Xavier. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lichtemberg, Paulo dos Santos Faria. University of California at Davis; Estados Unidos
Fil: Paredes, Juan Andres. University of California at Davis; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carraro, Thiago A.. University of California at Davis; Estados Unidos
Fil: Velasco, Jhordan. University of California at Davis; Estados Unidos
Fil: Michailides, Themis J.. University of California at Davis; Estados Unidos
description Dieback caused by Colletotrichum spp. is an emerging disease in California citrus groves. A large-scale survey with emphasis on seasonal variations of latent infections was conducted throughout citrus orchards in Fresno, Kern, and Tulare counties in 2019 and 2020. Latent infections on citrus leaves and twigs varied markedly between years. Isolates of Colletotrichum spp. were obtained from asymptomatic tissue and two groups were formed based on colony and spore morphology. The morphological groups were further identified based on multigene sequence analysis using the DNA regions ITS1-5.8S-ITS2, TUB2, and GAPDH. Results revealed that isolates belong to two phylogenetic species, C. gloeosporioides and C. karstii, being C. karstii more frequently isolated. Representative isolates of each species were further selected and characterized based on the response of physiological variables to temperature. Both species had similar optimum growth temperatures but differed in maximum growth rates, with C. gloeosporioides exhibiting a greater growth rate than that of C. karstii on media. Pathogenicity tests on citrus trees demonstrated the ability of C. gloeosporioides and C. karstii to cause lesions on twigs and no differences in aggressiveness. A fungicide screening performed in this study determined that the DMI fungicides were the most effective in reducing the mycelial growth of C. gloeosporioides and C. karstii. The QoI fungicides showed a remarkably inhibitory impact on spore germination of both species. On average, C. karstii was more sensitive to the DMI fungicides than C. gloeosporioides. The findings of this study provide new information to understand the Colletotrichum dieback of citrus.
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160393
Camiletti, Boris Xavier; Lichtemberg, Paulo dos Santos Faria; Paredes, Juan Andres; Carraro, Thiago A.; Velasco, Jhordan; et al.; Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California; American Phytopathological Society; Phytopathology; 6-2022; 1-43
0031-949X
1943-7684
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160393
identifier_str_mv Camiletti, Boris Xavier; Lichtemberg, Paulo dos Santos Faria; Paredes, Juan Andres; Carraro, Thiago A.; Velasco, Jhordan; et al.; Characterization of Colletotrichum isolates causing Colletotrichum dieback of citrus in California; American Phytopathological Society; Phytopathology; 6-2022; 1-43
0031-949X
1943-7684
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-10-21-0434-R
info:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-10-21-0434-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.665996

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