Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs

Autores
Fernandez, Jimena; Chamorro, Anahí; Herrera, Marcela Fernanda; Bianchi, Carolina Paula; Cueto, Marcela Isabel; Villar, Laura; Bruno Galarraga, Macarena
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The aim of the study was to describe the histomorphometric characteristics of the uterus of 45-days-old lambs belonging to mothersnutritionally restricted or controls from the second half of gestation.Merino pregnant ewes carrying single fetuses were randomly assigned to restricted (R) or control (C) groups and fed with 75% or125% of metabolic energy requirements from ~80 days to ~140days of gestation, respectively. Uterus samples from Merino female lambs of 45-days-old (n=7 for each group) were evaluated.For the histomorphometric evaluation, 3 cuts of 4 µm thickness ofeach uterine horns were obtained and stained with H-E in order toevaluate the following parameters: height of the luminal epithelium(HLE) and the glandular epithelium (HGE), considering the distancebetween the basal membrane and the apical end of 30 cells ofeach of the epithelia with a magnification of 100x. Glandular density (GDe), the number of glands in 10 fields at 40x magnificationand Glandular area (GA) using the following formula: GA=π×(Axis1)/2×(Axis 2)/2 were evaluated. The axes were the average of tworight-angled diameters in a minimum of 30 glands per section at 100x magnification. Samples were analyzed by two observers. Theresults were analyzed by ANOVA. Data are expressed as mean ±SD. Results were considered significant with P≤0.05. HLE and AGdid not differ between groups (R: 11.73±0.18; 1347.43±36.74 andC: 11.81±0.18; 1277.54±36.74; respectively; P≤0.05). The HGE andGDe were significantly higher for lambs from R than lambs from Cgroup (R: 13.34±0.18; 25.19±0.59; C: 12.86±0.18; 22.32±0.64; respectively; P≤0.05). The pre-natal maternal nutrition could affect thedevelopment of uterine glands in their lambs. In this study, a higherHE and GDe was observed in the uterus of lambs born from nutritional restricted mothers. Future studies are necessary to explainthese results.
Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Chamorro, Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Herrera, Marcela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Villar, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Bruno Galarraga, Macarena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
HISTOMORFOMETRÍA
ÚTERO
PROGRAMACIÓN FETAL
OVINOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253173
Acceder
id CONICETDig_4da7e33cf9bcb51681d753ed288beeba
oai_identifier_str oai:ri.conicet.gov.ar:11336/253173
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of histomorphometric changes in the uterus of prenatal restricted lambsFernandez, JimenaChamorro, AnahíHerrera, Marcela FernandaBianchi, Carolina PaulaCueto, Marcela IsabelVillar, LauraBruno Galarraga, MacarenaHISTOMORFOMETRÍAÚTEROPROGRAMACIÓN FETALOVINOShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the study was to describe the histomorphometric characteristics of the uterus of 45-days-old lambs belonging to mothersnutritionally restricted or controls from the second half of gestation.Merino pregnant ewes carrying single fetuses were randomly assigned to restricted (R) or control (C) groups and fed with 75% or125% of metabolic energy requirements from ~80 days to ~140days of gestation, respectively. Uterus samples from Merino female lambs of 45-days-old (n=7 for each group) were evaluated.For the histomorphometric evaluation, 3 cuts of 4 µm thickness ofeach uterine horns were obtained and stained with H-E in order toevaluate the following parameters: height of the luminal epithelium(HLE) and the glandular epithelium (HGE), considering the distancebetween the basal membrane and the apical end of 30 cells ofeach of the epithelia with a magnification of 100x. Glandular density (GDe), the number of glands in 10 fields at 40x magnificationand Glandular area (GA) using the following formula: GA=π×(Axis1)/2×(Axis 2)/2 were evaluated. The axes were the average of tworight-angled diameters in a minimum of 30 glands per section at 100x magnification. Samples were analyzed by two observers. Theresults were analyzed by ANOVA. Data are expressed as mean ±SD. Results were considered significant with P≤0.05. HLE and AGdid not differ between groups (R: 11.73±0.18; 1347.43±36.74 andC: 11.81±0.18; 1277.54±36.74; respectively; P≤0.05). The HGE andGDe were significantly higher for lambs from R than lambs from Cgroup (R: 13.34±0.18; 25.19±0.59; C: 12.86±0.18; 22.32±0.64; respectively; P≤0.05). The pre-natal maternal nutrition could affect thedevelopment of uterine glands in their lambs. In this study, a higherHE and GDe was observed in the uterus of lambs born from nutritional restricted mothers. Future studies are necessary to explainthese results.Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Chamorro, Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Herrera, Marcela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Villar, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Bruno Galarraga, Macarena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaSociedad Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253173Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 237-2371669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:24Zoai:ri.conicet.gov.ar:11336/253173instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:24.289CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
title Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
spellingShingle Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
Fernandez, Jimena
HISTOMORFOMETRÍA
ÚTERO
PROGRAMACIÓN FETAL
OVINOS
title_short Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
title_full Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
title_fullStr Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
title_full_unstemmed Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
title_sort Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs
dc.creator.none.fl_str_mv Fernandez, Jimena
Chamorro, Anahí
Herrera, Marcela Fernanda
Bianchi, Carolina Paula
Cueto, Marcela Isabel
Villar, Laura
Bruno Galarraga, Macarena
author Fernandez, Jimena
author_facet Fernandez, Jimena
Chamorro, Anahí
Herrera, Marcela Fernanda
Bianchi, Carolina Paula
Cueto, Marcela Isabel
Villar, Laura
Bruno Galarraga, Macarena
author_role author
author2 Chamorro, Anahí
Herrera, Marcela Fernanda
Bianchi, Carolina Paula
Cueto, Marcela Isabel
Villar, Laura
Bruno Galarraga, Macarena
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HISTOMORFOMETRÍA
ÚTERO
PROGRAMACIÓN FETAL
OVINOS
topic HISTOMORFOMETRÍA
ÚTERO
PROGRAMACIÓN FETAL
OVINOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the study was to describe the histomorphometric characteristics of the uterus of 45-days-old lambs belonging to mothersnutritionally restricted or controls from the second half of gestation.Merino pregnant ewes carrying single fetuses were randomly assigned to restricted (R) or control (C) groups and fed with 75% or125% of metabolic energy requirements from ~80 days to ~140days of gestation, respectively. Uterus samples from Merino female lambs of 45-days-old (n=7 for each group) were evaluated.For the histomorphometric evaluation, 3 cuts of 4 µm thickness ofeach uterine horns were obtained and stained with H-E in order toevaluate the following parameters: height of the luminal epithelium(HLE) and the glandular epithelium (HGE), considering the distancebetween the basal membrane and the apical end of 30 cells ofeach of the epithelia with a magnification of 100x. Glandular density (GDe), the number of glands in 10 fields at 40x magnificationand Glandular area (GA) using the following formula: GA=π×(Axis1)/2×(Axis 2)/2 were evaluated. The axes were the average of tworight-angled diameters in a minimum of 30 glands per section at 100x magnification. Samples were analyzed by two observers. Theresults were analyzed by ANOVA. Data are expressed as mean ±SD. Results were considered significant with P≤0.05. HLE and AGdid not differ between groups (R: 11.73±0.18; 1347.43±36.74 andC: 11.81±0.18; 1277.54±36.74; respectively; P≤0.05). The HGE andGDe were significantly higher for lambs from R than lambs from Cgroup (R: 13.34±0.18; 25.19±0.59; C: 12.86±0.18; 22.32±0.64; respectively; P≤0.05). The pre-natal maternal nutrition could affect thedevelopment of uterine glands in their lambs. In this study, a higherHE and GDe was observed in the uterus of lambs born from nutritional restricted mothers. Future studies are necessary to explainthese results.
Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Chamorro, Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Herrera, Marcela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Villar, Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Bruno Galarraga, Macarena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description The aim of the study was to describe the histomorphometric characteristics of the uterus of 45-days-old lambs belonging to mothersnutritionally restricted or controls from the second half of gestation.Merino pregnant ewes carrying single fetuses were randomly assigned to restricted (R) or control (C) groups and fed with 75% or125% of metabolic energy requirements from ~80 days to ~140days of gestation, respectively. Uterus samples from Merino female lambs of 45-days-old (n=7 for each group) were evaluated.For the histomorphometric evaluation, 3 cuts of 4 µm thickness ofeach uterine horns were obtained and stained with H-E in order toevaluate the following parameters: height of the luminal epithelium(HLE) and the glandular epithelium (HGE), considering the distancebetween the basal membrane and the apical end of 30 cells ofeach of the epithelia with a magnification of 100x. Glandular density (GDe), the number of glands in 10 fields at 40x magnificationand Glandular area (GA) using the following formula: GA=π×(Axis1)/2×(Axis 2)/2 were evaluated. The axes were the average of tworight-angled diameters in a minimum of 30 glands per section at 100x magnification. Samples were analyzed by two observers. Theresults were analyzed by ANOVA. Data are expressed as mean ±SD. Results were considered significant with P≤0.05. HLE and AGdid not differ between groups (R: 11.73±0.18; 1347.43±36.74 andC: 11.81±0.18; 1277.54±36.74; respectively; P≤0.05). The HGE andGDe were significantly higher for lambs from R than lambs from Cgroup (R: 13.34±0.18; 25.19±0.59; C: 12.86±0.18; 22.32±0.64; respectively; P≤0.05). The pre-natal maternal nutrition could affect thedevelopment of uterine glands in their lambs. In this study, a higherHE and GDe was observed in the uterus of lambs born from nutritional restricted mothers. Future studies are necessary to explainthese results.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253173
Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 237-237
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253173
identifier_str_mv Evaluation of histomorphometric changes in the uterus of prenatal restricted lambs; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 237-237
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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