Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors

Autores
Bernal Villegas, Aydiloide; la O, María de la Luz; Acevedo, Ricardo; Castillo, Ramiro; Gómez Kosky, Rafael; Nogueras, Aldo; Grellet Bournonville, Carlos Froilan; Castagnaro, Atilio Pedro; Daniels, Dion
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An alternative way to obtain molecules of high-value from plant origin for the agricultural and pharmaceutical industries is via the production of secondary metabolites from in vitro systems. Among these molecules are phenolic compounds that are important in plant defense system against biotic and abiotic factors. The objective of this work was to establish the culture conditions for the production and scaling up of phenolic compounds from sugarcane shoots produced in Temporary Immersion Bioreactors (TIBs). Two different sucrose concentrations and three inoculum densities of shoots per flask were tested for two sugarcane cultivars C86-56 and C1015-73. These cultivars were micropropagated in TIBs without ascorbic acid for phenolic compound production during 25 days, and were measured every five days. The best established conditions were evaluated on a pilot scale with ten TIBs for three sugarcane cultivars (C86-56, C1051-73 and C87-51). Although phenolic compounds were produced under all conditions evaluated at the experimental level, the most important production was achieved by using 15 shoots per vessel with 20 g.L-1 of sucrose in the absence of ascorbic acid after 25 days of culture. Cultivar C1051-73 had a higher multiplication coefficient independently of the treatments; this effect was not only significant for the phenolic compound production, but also for biomass increases. With respect to the pilot experiment, significant differences were detected for phenolic compound production among the three cultivars with C1051-73 showing the highest yield (23.05 mg.L-1). The results revealed that TIBs are a useful method to produce phenolic compounds with optimum yields.
Fil: Bernal Villegas, Aydiloide. Instituto de Investigaciones de la Caña de Azúcar; Cuba. Estación Territorial de Investigaciones de la Caña de Azúcar Centro Villa Clara; Cuba
Fil: la O, María de la Luz. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Acevedo, Ricardo. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Castillo, Ramiro. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Gómez Kosky, Rafael. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Nogueras, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Daniels, Dion. University of Belize; Belice
Materia
INVITRO CULTURE
PHENOLS
SUGARCANE
BIOREACTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260610
Acceder
id CONICETDig_4cb618d5e1c33f3f54585685f396a8ef
oai_identifier_str oai:ri.conicet.gov.ar:11336/260610
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactorsBernal Villegas, Aydiloidela O, María de la LuzAcevedo, RicardoCastillo, RamiroGómez Kosky, RafaelNogueras, AldoGrellet Bournonville, Carlos FroilanCastagnaro, Atilio PedroDaniels, DionINVITRO CULTUREPHENOLSSUGARCANEBIOREACTORShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4An alternative way to obtain molecules of high-value from plant origin for the agricultural and pharmaceutical industries is via the production of secondary metabolites from in vitro systems. Among these molecules are phenolic compounds that are important in plant defense system against biotic and abiotic factors. The objective of this work was to establish the culture conditions for the production and scaling up of phenolic compounds from sugarcane shoots produced in Temporary Immersion Bioreactors (TIBs). Two different sucrose concentrations and three inoculum densities of shoots per flask were tested for two sugarcane cultivars C86-56 and C1015-73. These cultivars were micropropagated in TIBs without ascorbic acid for phenolic compound production during 25 days, and were measured every five days. The best established conditions were evaluated on a pilot scale with ten TIBs for three sugarcane cultivars (C86-56, C1051-73 and C87-51). Although phenolic compounds were produced under all conditions evaluated at the experimental level, the most important production was achieved by using 15 shoots per vessel with 20 g.L-1 of sucrose in the absence of ascorbic acid after 25 days of culture. Cultivar C1051-73 had a higher multiplication coefficient independently of the treatments; this effect was not only significant for the phenolic compound production, but also for biomass increases. With respect to the pilot experiment, significant differences were detected for phenolic compound production among the three cultivars with C1051-73 showing the highest yield (23.05 mg.L-1). The results revealed that TIBs are a useful method to produce phenolic compounds with optimum yields.Fil: Bernal Villegas, Aydiloide. Instituto de Investigaciones de la Caña de Azúcar; Cuba. Estación Territorial de Investigaciones de la Caña de Azúcar Centro Villa Clara; CubaFil: la O, María de la Luz. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Acevedo, Ricardo. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Castillo, Ramiro. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Gómez Kosky, Rafael. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Nogueras, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Daniels, Dion. University of Belize; BeliceMedCrave2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260610Bernal Villegas, Aydiloide; la O, María de la Luz; Acevedo, Ricardo; Castillo, Ramiro; Gómez Kosky, Rafael; et al.; Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors; MedCrave; Horticulture International Journal; 8; 1; 1-2024; 1-62576-4462CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/HIJ/production-of-phenolic-compounds-from-in-vitro-shoots-of-sugarcane-saccharum-spp-in-temporary-immersion-bioreactors.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.15406/hij.2024.08.00294info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:04:29Zoai:ri.conicet.gov.ar:11336/260610instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:04:30.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
title Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
spellingShingle Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
Bernal Villegas, Aydiloide
INVITRO CULTURE
PHENOLS
SUGARCANE
BIOREACTORS
title_short Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
title_full Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
title_fullStr Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
title_full_unstemmed Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
title_sort Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors
dc.creator.none.fl_str_mv Bernal Villegas, Aydiloide
la O, María de la Luz
Acevedo, Ricardo
Castillo, Ramiro
Gómez Kosky, Rafael
Nogueras, Aldo
Grellet Bournonville, Carlos Froilan
Castagnaro, Atilio Pedro
Daniels, Dion
author Bernal Villegas, Aydiloide
author_facet Bernal Villegas, Aydiloide
la O, María de la Luz
Acevedo, Ricardo
Castillo, Ramiro
Gómez Kosky, Rafael
Nogueras, Aldo
Grellet Bournonville, Carlos Froilan
Castagnaro, Atilio Pedro
Daniels, Dion
author_role author
author2 la O, María de la Luz
Acevedo, Ricardo
Castillo, Ramiro
Gómez Kosky, Rafael
Nogueras, Aldo
Grellet Bournonville, Carlos Froilan
Castagnaro, Atilio Pedro
Daniels, Dion
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INVITRO CULTURE
PHENOLS
SUGARCANE
BIOREACTORS
topic INVITRO CULTURE
PHENOLS
SUGARCANE
BIOREACTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv An alternative way to obtain molecules of high-value from plant origin for the agricultural and pharmaceutical industries is via the production of secondary metabolites from in vitro systems. Among these molecules are phenolic compounds that are important in plant defense system against biotic and abiotic factors. The objective of this work was to establish the culture conditions for the production and scaling up of phenolic compounds from sugarcane shoots produced in Temporary Immersion Bioreactors (TIBs). Two different sucrose concentrations and three inoculum densities of shoots per flask were tested for two sugarcane cultivars C86-56 and C1015-73. These cultivars were micropropagated in TIBs without ascorbic acid for phenolic compound production during 25 days, and were measured every five days. The best established conditions were evaluated on a pilot scale with ten TIBs for three sugarcane cultivars (C86-56, C1051-73 and C87-51). Although phenolic compounds were produced under all conditions evaluated at the experimental level, the most important production was achieved by using 15 shoots per vessel with 20 g.L-1 of sucrose in the absence of ascorbic acid after 25 days of culture. Cultivar C1051-73 had a higher multiplication coefficient independently of the treatments; this effect was not only significant for the phenolic compound production, but also for biomass increases. With respect to the pilot experiment, significant differences were detected for phenolic compound production among the three cultivars with C1051-73 showing the highest yield (23.05 mg.L-1). The results revealed that TIBs are a useful method to produce phenolic compounds with optimum yields.
Fil: Bernal Villegas, Aydiloide. Instituto de Investigaciones de la Caña de Azúcar; Cuba. Estación Territorial de Investigaciones de la Caña de Azúcar Centro Villa Clara; Cuba
Fil: la O, María de la Luz. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Acevedo, Ricardo. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Castillo, Ramiro. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Gómez Kosky, Rafael. Instituto de Investigaciones de la Caña de Azúcar; Cuba
Fil: Nogueras, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Daniels, Dion. University of Belize; Belice
description An alternative way to obtain molecules of high-value from plant origin for the agricultural and pharmaceutical industries is via the production of secondary metabolites from in vitro systems. Among these molecules are phenolic compounds that are important in plant defense system against biotic and abiotic factors. The objective of this work was to establish the culture conditions for the production and scaling up of phenolic compounds from sugarcane shoots produced in Temporary Immersion Bioreactors (TIBs). Two different sucrose concentrations and three inoculum densities of shoots per flask were tested for two sugarcane cultivars C86-56 and C1015-73. These cultivars were micropropagated in TIBs without ascorbic acid for phenolic compound production during 25 days, and were measured every five days. The best established conditions were evaluated on a pilot scale with ten TIBs for three sugarcane cultivars (C86-56, C1051-73 and C87-51). Although phenolic compounds were produced under all conditions evaluated at the experimental level, the most important production was achieved by using 15 shoots per vessel with 20 g.L-1 of sucrose in the absence of ascorbic acid after 25 days of culture. Cultivar C1051-73 had a higher multiplication coefficient independently of the treatments; this effect was not only significant for the phenolic compound production, but also for biomass increases. With respect to the pilot experiment, significant differences were detected for phenolic compound production among the three cultivars with C1051-73 showing the highest yield (23.05 mg.L-1). The results revealed that TIBs are a useful method to produce phenolic compounds with optimum yields.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260610
Bernal Villegas, Aydiloide; la O, María de la Luz; Acevedo, Ricardo; Castillo, Ramiro; Gómez Kosky, Rafael; et al.; Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors; MedCrave; Horticulture International Journal; 8; 1; 1-2024; 1-6
2576-4462
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260610
identifier_str_mv Bernal Villegas, Aydiloide; la O, María de la Luz; Acevedo, Ricardo; Castillo, Ramiro; Gómez Kosky, Rafael; et al.; Production of phenolic compounds from in vitro shoots of sugarcane (Saccharum spp.) in temporary immersion bioreactors; MedCrave; Horticulture International Journal; 8; 1; 1-2024; 1-6
2576-4462
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medcraveonline.com/HIJ/production-of-phenolic-compounds-from-in-vitro-shoots-of-sugarcane-saccharum-spp-in-temporary-immersion-bioreactors.html
info:eu-repo/semantics/altIdentifier/doi/10.15406/hij.2024.08.00294
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MedCrave
publisher.none.fl_str_mv MedCrave
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980151096246272
score 12.993085

Publicaciones similares