Suspension-Vero cell cultures as a platform for viral vaccine production

Autores
Paillet, Cristian; Forno, Angela Guillermina; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.
Fil: Paillet, Cristian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Forno, Angela Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Materia
Vero Cells
Vaccines
Suspension culture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103974
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Suspension-Vero cell cultures as a platform for viral vaccine productionPaillet, CristianForno, Angela GuillerminaKratje, Ricardo BertoldoEtcheverrigaray, MarinaVero CellsVaccinesSuspension culturehttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.Fil: Paillet, Cristian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Forno, Angela Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaElsevier2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103974Paillet, Cristian; Forno, Angela Guillermina; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Suspension-Vero cell cultures as a platform for viral vaccine production; Elsevier; Vaccine; 27; 46; 10-2009; 6464-64670264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=1245480340&_sort=r&view=c&_acct=C000054198&_version=1&_urlVersion=0&_userid=3602825&md5=c7b0d5dfa81749494f9011f55c2c4e6dinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2009.06.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:05Zoai:ri.conicet.gov.ar:11336/103974instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:05.904CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Suspension-Vero cell cultures as a platform for viral vaccine production
title Suspension-Vero cell cultures as a platform for viral vaccine production
spellingShingle Suspension-Vero cell cultures as a platform for viral vaccine production
Paillet, Cristian
Vero Cells
Vaccines
Suspension culture
title_short Suspension-Vero cell cultures as a platform for viral vaccine production
title_full Suspension-Vero cell cultures as a platform for viral vaccine production
title_fullStr Suspension-Vero cell cultures as a platform for viral vaccine production
title_full_unstemmed Suspension-Vero cell cultures as a platform for viral vaccine production
title_sort Suspension-Vero cell cultures as a platform for viral vaccine production
dc.creator.none.fl_str_mv Paillet, Cristian
Forno, Angela Guillermina
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
author Paillet, Cristian
author_facet Paillet, Cristian
Forno, Angela Guillermina
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
author_role author
author2 Forno, Angela Guillermina
Kratje, Ricardo Bertoldo
Etcheverrigaray, Marina
author2_role author
author
author
dc.subject.none.fl_str_mv Vero Cells
Vaccines
Suspension culture
topic Vero Cells
Vaccines
Suspension culture
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.
Fil: Paillet, Cristian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Forno, Angela Guillermina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
description Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103974
Paillet, Cristian; Forno, Angela Guillermina; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Suspension-Vero cell cultures as a platform for viral vaccine production; Elsevier; Vaccine; 27; 46; 10-2009; 6464-6467
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103974
identifier_str_mv Paillet, Cristian; Forno, Angela Guillermina; Kratje, Ricardo Bertoldo; Etcheverrigaray, Marina; Suspension-Vero cell cultures as a platform for viral vaccine production; Elsevier; Vaccine; 27; 46; 10-2009; 6464-6467
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=1245480340&_sort=r&view=c&_acct=C000054198&_version=1&_urlVersion=0&_userid=3602825&md5=c7b0d5dfa81749494f9011f55c2c4e6d
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2009.06.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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