Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol

Autores
Daddario, Juan Facundo Fabian; Bianchinotti, Maria Virginia; Anderson, Freda Elizabeth
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Saltcedars (Tamarix spp.), are among the invasive alien shrubs and trees present in Argentina causing major ecological and economic problems. As such, they have been selected as one of the pilot programmes of the National Strategy on Invasive Alien Species. A biological control (BC) project against Tamarix spp. was initiated in 2017, funded by the Ministry of Science, Technology and Productive Innovation with the main objective of assessing the feasibility of introducing the defoliating and stem damaging beetle Diorhabda sublineata, a BC agent successfully released against Tamarix spp. in the USA. It is common practice for these projects, to record the organisms associated with the target in its adventive range, to identify any that may either interfere with the BC agents intended for release, or that may themselves have potential as such. To this end, surveys were conducted to search for fungal pathogens in Tamarix populations in Argentina. T. ramosissima trees showing discoloration and death of branches were found at two sites located around 700 km apart. Several fungi were identified associated with these symptoms, among which Cytospora unilocularis and Neomicrophaeropsis tamaricicola, appeared as the most likely causal agents. Both were isolated on artificial culture media to provide inoculum for pathogenicity tests. Branches of six-month-old T. ramosissima potted plants were inoculated with C. unilocularis by either placing a drop of a conidial suspension in water into ad-hoc produced wounds or by spraying undamaged ones. Inoculation with N. tamaricicola was performed only on wounded branches. Control plants were treated with water alone. Wounded plants inoculated with C. unilocularis developed cankers, branch discoloration and death of branches after two months. Pycnidia in association with the symptoms were also observed on one of the plants. The fungus was re-isolated from the cankers allowing for the completion of Koch's postulates. To our knowledge, this is the first report of C. unilocularis causing disease on T. ramosissima. In this ongoing experiment, incipient symptoms have developed so far on the plants inoculated with N. tamaricicola. Both fungi have recently been described from the plant´s native range, suggesting they must have arrived in Argentina together with their host. Because C. unilocularis seems to infect only through wounds, the effect of this pathogen would be enhanced should D. sublineata eventually be introduced, thus contributing to a better control of populations where the pathogen is already present.
Fil: Daddario, Juan Facundo Fabian. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
XVI International Symposium on Biological Control of Weeds
Puerto Iguazú
Argentina
Fundación para el estudio de especies invasivas
Centro de Recursos Naturales Renovables de la Zona Semiárida
Materia
WOOD CANKER
BRANCH DEATH
BIOLOGICAL CONTROL
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258319
Acceder
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spelling Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrolDaddario, Juan Facundo FabianBianchinotti, Maria VirginiaAnderson, Freda ElizabethWOOD CANKERBRANCH DEATHBIOLOGICAL CONTROLARGENTINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Saltcedars (Tamarix spp.), are among the invasive alien shrubs and trees present in Argentina causing major ecological and economic problems. As such, they have been selected as one of the pilot programmes of the National Strategy on Invasive Alien Species. A biological control (BC) project against Tamarix spp. was initiated in 2017, funded by the Ministry of Science, Technology and Productive Innovation with the main objective of assessing the feasibility of introducing the defoliating and stem damaging beetle Diorhabda sublineata, a BC agent successfully released against Tamarix spp. in the USA. It is common practice for these projects, to record the organisms associated with the target in its adventive range, to identify any that may either interfere with the BC agents intended for release, or that may themselves have potential as such. To this end, surveys were conducted to search for fungal pathogens in Tamarix populations in Argentina. T. ramosissima trees showing discoloration and death of branches were found at two sites located around 700 km apart. Several fungi were identified associated with these symptoms, among which Cytospora unilocularis and Neomicrophaeropsis tamaricicola, appeared as the most likely causal agents. Both were isolated on artificial culture media to provide inoculum for pathogenicity tests. Branches of six-month-old T. ramosissima potted plants were inoculated with C. unilocularis by either placing a drop of a conidial suspension in water into ad-hoc produced wounds or by spraying undamaged ones. Inoculation with N. tamaricicola was performed only on wounded branches. Control plants were treated with water alone. Wounded plants inoculated with C. unilocularis developed cankers, branch discoloration and death of branches after two months. Pycnidia in association with the symptoms were also observed on one of the plants. The fungus was re-isolated from the cankers allowing for the completion of Koch's postulates. To our knowledge, this is the first report of C. unilocularis causing disease on T. ramosissima. In this ongoing experiment, incipient symptoms have developed so far on the plants inoculated with N. tamaricicola. Both fungi have recently been described from the plant´s native range, suggesting they must have arrived in Argentina together with their host. Because C. unilocularis seems to infect only through wounds, the effect of this pathogen would be enhanced should D. sublineata eventually be introduced, thus contributing to a better control of populations where the pathogen is already present.Fil: Daddario, Juan Facundo Fabian. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaXVI International Symposium on Biological Control of WeedsPuerto IguazúArgentinaFundación para el estudio de especies invasivasCentro de Recursos Naturales Renovables de la Zona SemiáridaInternational Symposium on biological control of weeds2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258319Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol; XVI International Symposium on Biological Control of Weeds; Puerto Iguazú; Argentina; 2023; 100-100CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.iobc-global.org/download/20230507-12_ISBCW_Iguazu_Proceedings.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:00Zoai:ri.conicet.gov.ar:11336/258319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:00.496CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
title Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
spellingShingle Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
Daddario, Juan Facundo Fabian
WOOD CANKER
BRANCH DEATH
BIOLOGICAL CONTROL
ARGENTINA
title_short Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
title_full Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
title_fullStr Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
title_full_unstemmed Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
title_sort Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol
dc.creator.none.fl_str_mv Daddario, Juan Facundo Fabian
Bianchinotti, Maria Virginia
Anderson, Freda Elizabeth
author Daddario, Juan Facundo Fabian
author_facet Daddario, Juan Facundo Fabian
Bianchinotti, Maria Virginia
Anderson, Freda Elizabeth
author_role author
author2 Bianchinotti, Maria Virginia
Anderson, Freda Elizabeth
author2_role author
author
dc.subject.none.fl_str_mv WOOD CANKER
BRANCH DEATH
BIOLOGICAL CONTROL
ARGENTINA
topic WOOD CANKER
BRANCH DEATH
BIOLOGICAL CONTROL
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Saltcedars (Tamarix spp.), are among the invasive alien shrubs and trees present in Argentina causing major ecological and economic problems. As such, they have been selected as one of the pilot programmes of the National Strategy on Invasive Alien Species. A biological control (BC) project against Tamarix spp. was initiated in 2017, funded by the Ministry of Science, Technology and Productive Innovation with the main objective of assessing the feasibility of introducing the defoliating and stem damaging beetle Diorhabda sublineata, a BC agent successfully released against Tamarix spp. in the USA. It is common practice for these projects, to record the organisms associated with the target in its adventive range, to identify any that may either interfere with the BC agents intended for release, or that may themselves have potential as such. To this end, surveys were conducted to search for fungal pathogens in Tamarix populations in Argentina. T. ramosissima trees showing discoloration and death of branches were found at two sites located around 700 km apart. Several fungi were identified associated with these symptoms, among which Cytospora unilocularis and Neomicrophaeropsis tamaricicola, appeared as the most likely causal agents. Both were isolated on artificial culture media to provide inoculum for pathogenicity tests. Branches of six-month-old T. ramosissima potted plants were inoculated with C. unilocularis by either placing a drop of a conidial suspension in water into ad-hoc produced wounds or by spraying undamaged ones. Inoculation with N. tamaricicola was performed only on wounded branches. Control plants were treated with water alone. Wounded plants inoculated with C. unilocularis developed cankers, branch discoloration and death of branches after two months. Pycnidia in association with the symptoms were also observed on one of the plants. The fungus was re-isolated from the cankers allowing for the completion of Koch's postulates. To our knowledge, this is the first report of C. unilocularis causing disease on T. ramosissima. In this ongoing experiment, incipient symptoms have developed so far on the plants inoculated with N. tamaricicola. Both fungi have recently been described from the plant´s native range, suggesting they must have arrived in Argentina together with their host. Because C. unilocularis seems to infect only through wounds, the effect of this pathogen would be enhanced should D. sublineata eventually be introduced, thus contributing to a better control of populations where the pathogen is already present.
Fil: Daddario, Juan Facundo Fabian. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Anderson, Freda Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
XVI International Symposium on Biological Control of Weeds
Puerto Iguazú
Argentina
Fundación para el estudio de especies invasivas
Centro de Recursos Naturales Renovables de la Zona Semiárida
description Saltcedars (Tamarix spp.), are among the invasive alien shrubs and trees present in Argentina causing major ecological and economic problems. As such, they have been selected as one of the pilot programmes of the National Strategy on Invasive Alien Species. A biological control (BC) project against Tamarix spp. was initiated in 2017, funded by the Ministry of Science, Technology and Productive Innovation with the main objective of assessing the feasibility of introducing the defoliating and stem damaging beetle Diorhabda sublineata, a BC agent successfully released against Tamarix spp. in the USA. It is common practice for these projects, to record the organisms associated with the target in its adventive range, to identify any that may either interfere with the BC agents intended for release, or that may themselves have potential as such. To this end, surveys were conducted to search for fungal pathogens in Tamarix populations in Argentina. T. ramosissima trees showing discoloration and death of branches were found at two sites located around 700 km apart. Several fungi were identified associated with these symptoms, among which Cytospora unilocularis and Neomicrophaeropsis tamaricicola, appeared as the most likely causal agents. Both were isolated on artificial culture media to provide inoculum for pathogenicity tests. Branches of six-month-old T. ramosissima potted plants were inoculated with C. unilocularis by either placing a drop of a conidial suspension in water into ad-hoc produced wounds or by spraying undamaged ones. Inoculation with N. tamaricicola was performed only on wounded branches. Control plants were treated with water alone. Wounded plants inoculated with C. unilocularis developed cankers, branch discoloration and death of branches after two months. Pycnidia in association with the symptoms were also observed on one of the plants. The fungus was re-isolated from the cankers allowing for the completion of Koch's postulates. To our knowledge, this is the first report of C. unilocularis causing disease on T. ramosissima. In this ongoing experiment, incipient symptoms have developed so far on the plants inoculated with N. tamaricicola. Both fungi have recently been described from the plant´s native range, suggesting they must have arrived in Argentina together with their host. Because C. unilocularis seems to infect only through wounds, the effect of this pathogen would be enhanced should D. sublineata eventually be introduced, thus contributing to a better control of populations where the pathogen is already present.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258319
Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol; XVI International Symposium on Biological Control of Weeds; Puerto Iguazú; Argentina; 2023; 100-100
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258319
identifier_str_mv Tamarix ramosissima wood canker and branch death in Argentina: assessment of the role of associated fungi and possible implications for classical biocontrol; XVI International Symposium on Biological Control of Weeds; Puerto Iguazú; Argentina; 2023; 100-100
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.iobc-global.org/download/20230507-12_ISBCW_Iguazu_Proceedings.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Symposium on biological control of weeds
publisher.none.fl_str_mv International Symposium on biological control of weeds
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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