Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation

Autores
Ramirez, Daniela Andrea; Beretta, Hebe Vanesa; Torres Palazzolo, Carolina Andrea; Camargo, Alejandra Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Radishes (Raphanus sativus L.) are cruciferous vegetables with remarkable nutraceutical properties given their distinctive isothiocyanates (ITCs) profile. These compounds are formed after glucosinolates-Myrosinase enzymolysis. Although it is important to characterize radishes´ ITCs levels, it is also necessary to evaluate the bioactive compounds´ physiological fate after radishes ingestion. To do so, the extraction techniques should adapt to such conditions of high aqueous environment. In this work, we studied the bioaccessibility of ITCs and indole-3-carbinol (I3C) in radishes taproots considering the analytical implications of this biological process. Results showed that ITCs and indole profiles in the radish´s taproots after aqueous-myrosinase hydrolysis followed by Dispersive Liquid-Liquid Microextraction were distinctively different from other reports. After in vitro digestion, raphasatin showed the highest bioaccessibility despite its low quantitative yields. Notably, I3C and S-Sulforaphene become promising phytochemicals, due to their bioaccessibility and their considerable remaining amounts after digestion.
Fil: Ramirez, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Torres Palazzolo, Carolina Andrea. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
ANALITYCAL METHODOLOGY
BIOACCESSIBILITY
ISOTHIOCYANATES
RADISHES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/222271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretationRamirez, Daniela AndreaBeretta, Hebe VanesaTorres Palazzolo, Carolina AndreaCamargo, Alejandra BeatrizANALITYCAL METHODOLOGYBIOACCESSIBILITYISOTHIOCYANATESRADISHEShttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Radishes (Raphanus sativus L.) are cruciferous vegetables with remarkable nutraceutical properties given their distinctive isothiocyanates (ITCs) profile. These compounds are formed after glucosinolates-Myrosinase enzymolysis. Although it is important to characterize radishes´ ITCs levels, it is also necessary to evaluate the bioactive compounds´ physiological fate after radishes ingestion. To do so, the extraction techniques should adapt to such conditions of high aqueous environment. In this work, we studied the bioaccessibility of ITCs and indole-3-carbinol (I3C) in radishes taproots considering the analytical implications of this biological process. Results showed that ITCs and indole profiles in the radish´s taproots after aqueous-myrosinase hydrolysis followed by Dispersive Liquid-Liquid Microextraction were distinctively different from other reports. After in vitro digestion, raphasatin showed the highest bioaccessibility despite its low quantitative yields. Notably, I3C and S-Sulforaphene become promising phytochemicals, due to their bioaccessibility and their considerable remaining amounts after digestion.Fil: Ramirez, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Torres Palazzolo, Carolina Andrea. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaWiley2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222271Ramirez, Daniela Andrea; Beretta, Hebe Vanesa; Torres Palazzolo, Carolina Andrea; Camargo, Alejandra Beatriz; Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation; Wiley; Food Safety and Health; 7-2023; 1-92835-10962835-1096CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/fsh3.12014info:eu-repo/semantics/altIdentifier/doi/10.1002/fsh3.12014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:23Zoai:ri.conicet.gov.ar:11336/222271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:23.836CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
title Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
spellingShingle Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
Ramirez, Daniela Andrea
ANALITYCAL METHODOLOGY
BIOACCESSIBILITY
ISOTHIOCYANATES
RADISHES
title_short Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
title_full Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
title_fullStr Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
title_full_unstemmed Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
title_sort Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation
dc.creator.none.fl_str_mv Ramirez, Daniela Andrea
Beretta, Hebe Vanesa
Torres Palazzolo, Carolina Andrea
Camargo, Alejandra Beatriz
author Ramirez, Daniela Andrea
author_facet Ramirez, Daniela Andrea
Beretta, Hebe Vanesa
Torres Palazzolo, Carolina Andrea
Camargo, Alejandra Beatriz
author_role author
author2 Beretta, Hebe Vanesa
Torres Palazzolo, Carolina Andrea
Camargo, Alejandra Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv ANALITYCAL METHODOLOGY
BIOACCESSIBILITY
ISOTHIOCYANATES
RADISHES
topic ANALITYCAL METHODOLOGY
BIOACCESSIBILITY
ISOTHIOCYANATES
RADISHES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Radishes (Raphanus sativus L.) are cruciferous vegetables with remarkable nutraceutical properties given their distinctive isothiocyanates (ITCs) profile. These compounds are formed after glucosinolates-Myrosinase enzymolysis. Although it is important to characterize radishes´ ITCs levels, it is also necessary to evaluate the bioactive compounds´ physiological fate after radishes ingestion. To do so, the extraction techniques should adapt to such conditions of high aqueous environment. In this work, we studied the bioaccessibility of ITCs and indole-3-carbinol (I3C) in radishes taproots considering the analytical implications of this biological process. Results showed that ITCs and indole profiles in the radish´s taproots after aqueous-myrosinase hydrolysis followed by Dispersive Liquid-Liquid Microextraction were distinctively different from other reports. After in vitro digestion, raphasatin showed the highest bioaccessibility despite its low quantitative yields. Notably, I3C and S-Sulforaphene become promising phytochemicals, due to their bioaccessibility and their considerable remaining amounts after digestion.
Fil: Ramirez, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Torres Palazzolo, Carolina Andrea. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Radishes (Raphanus sativus L.) are cruciferous vegetables with remarkable nutraceutical properties given their distinctive isothiocyanates (ITCs) profile. These compounds are formed after glucosinolates-Myrosinase enzymolysis. Although it is important to characterize radishes´ ITCs levels, it is also necessary to evaluate the bioactive compounds´ physiological fate after radishes ingestion. To do so, the extraction techniques should adapt to such conditions of high aqueous environment. In this work, we studied the bioaccessibility of ITCs and indole-3-carbinol (I3C) in radishes taproots considering the analytical implications of this biological process. Results showed that ITCs and indole profiles in the radish´s taproots after aqueous-myrosinase hydrolysis followed by Dispersive Liquid-Liquid Microextraction were distinctively different from other reports. After in vitro digestion, raphasatin showed the highest bioaccessibility despite its low quantitative yields. Notably, I3C and S-Sulforaphene become promising phytochemicals, due to their bioaccessibility and their considerable remaining amounts after digestion.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222271
Ramirez, Daniela Andrea; Beretta, Hebe Vanesa; Torres Palazzolo, Carolina Andrea; Camargo, Alejandra Beatriz; Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation; Wiley; Food Safety and Health; 7-2023; 1-9
2835-1096
2835-1096
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222271
identifier_str_mv Ramirez, Daniela Andrea; Beretta, Hebe Vanesa; Torres Palazzolo, Carolina Andrea; Camargo, Alejandra Beatriz; Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation; Wiley; Food Safety and Health; 7-2023; 1-9
2835-1096
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/fsh3.12014
info:eu-repo/semantics/altIdentifier/doi/10.1002/fsh3.12014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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