Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics

Autores
Torres, Carola Analía; Nuñez, María Beatriz; Isla, Maria Ines; Castro, Marcela Paola; González, Ana María; Zampini, Iris Catiana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Context: Some species of Bignoniaceae are widely used in medicinal practice by the natives of South America. Aims: Tinctures and infusions from twelve tree species of this family were evaluated for in vitro antibacterial activity against pathogenic bacteria. The effect of interactions between the four most active extracts and conventional antibiotics was also evaluated. Methods and Material: Bioautography and disc diffusion methods were used to select the most active extracts, then agar macrodilution and broth microdilution method were used to determine the minimal inhibitory and minimal bactericidal concentration (MIC and MBC). Time-kill assay and checkerboard method were employed to determine the type of antimicrobial effect and synergism, respectively. Results: It could be determined that tinctures from Catalpa bignonioides, Handroanthus pulcherrimus, Tabebuia nodosa and Tecoma stans were able to inhibit bacterial growth. The MIC and MBC observed were between 125-1000 μg GAE/ml and 500-1000 μg GAE/ml, respectively. The tested extracts were more effective against Gram-positive microorganisms. Time-kill experiments indicated bacteriostatic activity. Phytochemical screening showed terpenoids,phenols and flavonoids. Alkaloids were detected only in Tecoma stans. Among these combinations, the best was Tabebuia nodosa extract plus gentamicin. In most cases, MIC values were reduced 16-32 times for antibiotics, and even 8-16 times for extracts. Conclusion: These results revealed that some of the selected combinations could efficiently inhibit the growth of tested strains at lower concentrations than those required for the lonely use of the antimicrobial. These extracts would improve the efficacy of Antibiotics against resistant bacteria, hence they could be used for anti-infective therapy.
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Castro, Marcela Paola. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Materia
CHECKERBOARD METHOD
FRACTIONAL INHIBITORY CONCENTRATION
GENTAMICIN
TABEBUIA NODOSA
TIME KILL EXPERIMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27078
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/27078
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibioticsTorres, Carola AnalíaNuñez, María BeatrizIsla, Maria InesCastro, Marcela PaolaGonzález, Ana MaríaZampini, Iris CatianaCHECKERBOARD METHODFRACTIONAL INHIBITORY CONCENTRATIONGENTAMICINTABEBUIA NODOSATIME KILL EXPERIMENTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Context: Some species of Bignoniaceae are widely used in medicinal practice by the natives of South America. Aims: Tinctures and infusions from twelve tree species of this family were evaluated for in vitro antibacterial activity against pathogenic bacteria. The effect of interactions between the four most active extracts and conventional antibiotics was also evaluated. Methods and Material: Bioautography and disc diffusion methods were used to select the most active extracts, then agar macrodilution and broth microdilution method were used to determine the minimal inhibitory and minimal bactericidal concentration (MIC and MBC). Time-kill assay and checkerboard method were employed to determine the type of antimicrobial effect and synergism, respectively. Results: It could be determined that tinctures from Catalpa bignonioides, Handroanthus pulcherrimus, Tabebuia nodosa and Tecoma stans were able to inhibit bacterial growth. The MIC and MBC observed were between 125-1000 μg GAE/ml and 500-1000 μg GAE/ml, respectively. The tested extracts were more effective against Gram-positive microorganisms. Time-kill experiments indicated bacteriostatic activity. Phytochemical screening showed terpenoids,phenols and flavonoids. Alkaloids were detected only in Tecoma stans. Among these combinations, the best was Tabebuia nodosa extract plus gentamicin. In most cases, MIC values were reduced 16-32 times for antibiotics, and even 8-16 times for extracts. Conclusion: These results revealed that some of the selected combinations could efficiently inhibit the growth of tested strains at lower concentrations than those required for the lonely use of the antimicrobial. These extracts would improve the efficacy of Antibiotics against resistant bacteria, hence they could be used for anti-infective therapy.Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Castro, Marcela Paola. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaPharmacognosy Network Worldwide2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27078Torres, Carola Analía; Nuñez, María Beatriz; Isla, Maria Ines; Castro, Marcela Paola; González, Ana María; et al.; Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics; Pharmacognosy Network Worldwide; Pharmacognosy Journal; 7; 6; 12-2015; 400-4050975-3575CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.phcogj.com/article/84info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:44:12Zoai:ri.conicet.gov.ar:11336/27078instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:44:12.62CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
title Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
spellingShingle Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
Torres, Carola Analía
CHECKERBOARD METHOD
FRACTIONAL INHIBITORY CONCENTRATION
GENTAMICIN
TABEBUIA NODOSA
TIME KILL EXPERIMENTS
title_short Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
title_full Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
title_fullStr Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
title_full_unstemmed Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
title_sort Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics
dc.creator.none.fl_str_mv Torres, Carola Analía
Nuñez, María Beatriz
Isla, Maria Ines
Castro, Marcela Paola
González, Ana María
Zampini, Iris Catiana
author Torres, Carola Analía
author_facet Torres, Carola Analía
Nuñez, María Beatriz
Isla, Maria Ines
Castro, Marcela Paola
González, Ana María
Zampini, Iris Catiana
author_role author
author2 Nuñez, María Beatriz
Isla, Maria Ines
Castro, Marcela Paola
González, Ana María
Zampini, Iris Catiana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CHECKERBOARD METHOD
FRACTIONAL INHIBITORY CONCENTRATION
GENTAMICIN
TABEBUIA NODOSA
TIME KILL EXPERIMENTS
topic CHECKERBOARD METHOD
FRACTIONAL INHIBITORY CONCENTRATION
GENTAMICIN
TABEBUIA NODOSA
TIME KILL EXPERIMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Context: Some species of Bignoniaceae are widely used in medicinal practice by the natives of South America. Aims: Tinctures and infusions from twelve tree species of this family were evaluated for in vitro antibacterial activity against pathogenic bacteria. The effect of interactions between the four most active extracts and conventional antibiotics was also evaluated. Methods and Material: Bioautography and disc diffusion methods were used to select the most active extracts, then agar macrodilution and broth microdilution method were used to determine the minimal inhibitory and minimal bactericidal concentration (MIC and MBC). Time-kill assay and checkerboard method were employed to determine the type of antimicrobial effect and synergism, respectively. Results: It could be determined that tinctures from Catalpa bignonioides, Handroanthus pulcherrimus, Tabebuia nodosa and Tecoma stans were able to inhibit bacterial growth. The MIC and MBC observed were between 125-1000 μg GAE/ml and 500-1000 μg GAE/ml, respectively. The tested extracts were more effective against Gram-positive microorganisms. Time-kill experiments indicated bacteriostatic activity. Phytochemical screening showed terpenoids,phenols and flavonoids. Alkaloids were detected only in Tecoma stans. Among these combinations, the best was Tabebuia nodosa extract plus gentamicin. In most cases, MIC values were reduced 16-32 times for antibiotics, and even 8-16 times for extracts. Conclusion: These results revealed that some of the selected combinations could efficiently inhibit the growth of tested strains at lower concentrations than those required for the lonely use of the antimicrobial. These extracts would improve the efficacy of Antibiotics against resistant bacteria, hence they could be used for anti-infective therapy.
Fil: Torres, Carola Analía. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nuñez, María Beatriz. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Castro, Marcela Paola. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
description Context: Some species of Bignoniaceae are widely used in medicinal practice by the natives of South America. Aims: Tinctures and infusions from twelve tree species of this family were evaluated for in vitro antibacterial activity against pathogenic bacteria. The effect of interactions between the four most active extracts and conventional antibiotics was also evaluated. Methods and Material: Bioautography and disc diffusion methods were used to select the most active extracts, then agar macrodilution and broth microdilution method were used to determine the minimal inhibitory and minimal bactericidal concentration (MIC and MBC). Time-kill assay and checkerboard method were employed to determine the type of antimicrobial effect and synergism, respectively. Results: It could be determined that tinctures from Catalpa bignonioides, Handroanthus pulcherrimus, Tabebuia nodosa and Tecoma stans were able to inhibit bacterial growth. The MIC and MBC observed were between 125-1000 μg GAE/ml and 500-1000 μg GAE/ml, respectively. The tested extracts were more effective against Gram-positive microorganisms. Time-kill experiments indicated bacteriostatic activity. Phytochemical screening showed terpenoids,phenols and flavonoids. Alkaloids were detected only in Tecoma stans. Among these combinations, the best was Tabebuia nodosa extract plus gentamicin. In most cases, MIC values were reduced 16-32 times for antibiotics, and even 8-16 times for extracts. Conclusion: These results revealed that some of the selected combinations could efficiently inhibit the growth of tested strains at lower concentrations than those required for the lonely use of the antimicrobial. These extracts would improve the efficacy of Antibiotics against resistant bacteria, hence they could be used for anti-infective therapy.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27078
Torres, Carola Analía; Nuñez, María Beatriz; Isla, Maria Ines; Castro, Marcela Paola; González, Ana María; et al.; Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics; Pharmacognosy Network Worldwide; Pharmacognosy Journal; 7; 6; 12-2015; 400-405
0975-3575
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27078
identifier_str_mv Torres, Carola Analía; Nuñez, María Beatriz; Isla, Maria Ines; Castro, Marcela Paola; González, Ana María; et al.; Antibacterial activity of tinctures from Tree leaves belonging to the Bignoniaceae family and their synergistic effect with antibiotics; Pharmacognosy Network Worldwide; Pharmacognosy Journal; 7; 6; 12-2015; 400-405
0975-3575
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.phcogj.com/article/84
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pharmacognosy Network Worldwide
publisher.none.fl_str_mv Pharmacognosy Network Worldwide
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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