Long term stability and infectivity of herpesviruses in water

Autores
Dayaram, Anisha; Franz, Mathias; Schattschneider, Alexander; Damiani, Armando Mario; Bischofberger, Sebastian; Osterrieder, Nikolaus; Greenwood, Alex D.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
For viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. There are many factors including pH, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. Equine herpesvirus type-1 (EHV-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. EHV-1 and recombinants of EHV-1 and EHV-9 are able to cause infections in non-equid animal species, particularly in captive settings. Many of the captive non-equid mammals are not naturally sympatric with equids and do not share enclosures, however, in many cases water sources may overlap. Similarly, in the wild, equids encounter many species at waterholes in times of seasonal drought. Therefore, we hypothesized that EHV-1 is stable in water and that water may act as a vector for EHV-1. In order to establish the conditions promoting or hindering EHV-1 longevity, infectivity and genomic stability in water; we exposed EHV-1 to varied water environments (pH, salinity, temperature, and turbidity) in controlled experiments over 21 days. The presence and infectivity of the virus was confirmed by both qPCR and cell culture experiments. Our results show that EHV-1 remains stable and infectious under many conditions in water for up to three weeks.
Fil: Dayaram, Anisha. Leibniz Institute For Zoo And Wildlife Research; Alemania
Fil: Franz, Mathias. Leibniz-institute For Zoo And Wildlife Research; Alemania
Fil: Schattschneider, Alexander. Leibniz-institute For Zoo And Wildlife Research; Alemania
Fil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Freie University Berlin; Alemania
Fil: Bischofberger, Sebastian. Freie University Berlin; Alemania
Fil: Osterrieder, Nikolaus. Freie University Berlin; Alemania
Fil: Greenwood, Alex D.. Leibniz-institute For Zoo And Wildlife Research; Alemania
Materia
EHV-1
stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49746
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49746
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long term stability and infectivity of herpesviruses in waterDayaram, AnishaFranz, MathiasSchattschneider, AlexanderDamiani, Armando MarioBischofberger, SebastianOsterrieder, NikolausGreenwood, Alex D.EHV-1stabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1For viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. There are many factors including pH, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. Equine herpesvirus type-1 (EHV-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. EHV-1 and recombinants of EHV-1 and EHV-9 are able to cause infections in non-equid animal species, particularly in captive settings. Many of the captive non-equid mammals are not naturally sympatric with equids and do not share enclosures, however, in many cases water sources may overlap. Similarly, in the wild, equids encounter many species at waterholes in times of seasonal drought. Therefore, we hypothesized that EHV-1 is stable in water and that water may act as a vector for EHV-1. In order to establish the conditions promoting or hindering EHV-1 longevity, infectivity and genomic stability in water; we exposed EHV-1 to varied water environments (pH, salinity, temperature, and turbidity) in controlled experiments over 21 days. The presence and infectivity of the virus was confirmed by both qPCR and cell culture experiments. Our results show that EHV-1 remains stable and infectious under many conditions in water for up to three weeks.Fil: Dayaram, Anisha. Leibniz Institute For Zoo And Wildlife Research; AlemaniaFil: Franz, Mathias. Leibniz-institute For Zoo And Wildlife Research; AlemaniaFil: Schattschneider, Alexander. Leibniz-institute For Zoo And Wildlife Research; AlemaniaFil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Freie University Berlin; AlemaniaFil: Bischofberger, Sebastian. Freie University Berlin; AlemaniaFil: Osterrieder, Nikolaus. Freie University Berlin; AlemaniaFil: Greenwood, Alex D.. Leibniz-institute For Zoo And Wildlife Research; AlemaniaNature Publishing Group2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49746Dayaram, Anisha; Franz, Mathias; Schattschneider, Alexander; Damiani, Armando Mario; Bischofberger, Sebastian; et al.; Long term stability and infectivity of herpesviruses in water; Nature Publishing Group; Scientific Reports; 7; 4-2017; 1-102045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/srep46559info:eu-repo/semantics/altIdentifier/doi/10.1038/srep46559info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:05:13Zoai:ri.conicet.gov.ar:11336/49746instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:05:14.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long term stability and infectivity of herpesviruses in water
title Long term stability and infectivity of herpesviruses in water
spellingShingle Long term stability and infectivity of herpesviruses in water
Dayaram, Anisha
EHV-1
stability
title_short Long term stability and infectivity of herpesviruses in water
title_full Long term stability and infectivity of herpesviruses in water
title_fullStr Long term stability and infectivity of herpesviruses in water
title_full_unstemmed Long term stability and infectivity of herpesviruses in water
title_sort Long term stability and infectivity of herpesviruses in water
dc.creator.none.fl_str_mv Dayaram, Anisha
Franz, Mathias
Schattschneider, Alexander
Damiani, Armando Mario
Bischofberger, Sebastian
Osterrieder, Nikolaus
Greenwood, Alex D.
author Dayaram, Anisha
author_facet Dayaram, Anisha
Franz, Mathias
Schattschneider, Alexander
Damiani, Armando Mario
Bischofberger, Sebastian
Osterrieder, Nikolaus
Greenwood, Alex D.
author_role author
author2 Franz, Mathias
Schattschneider, Alexander
Damiani, Armando Mario
Bischofberger, Sebastian
Osterrieder, Nikolaus
Greenwood, Alex D.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EHV-1
stability
topic EHV-1
stability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv For viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. There are many factors including pH, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. Equine herpesvirus type-1 (EHV-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. EHV-1 and recombinants of EHV-1 and EHV-9 are able to cause infections in non-equid animal species, particularly in captive settings. Many of the captive non-equid mammals are not naturally sympatric with equids and do not share enclosures, however, in many cases water sources may overlap. Similarly, in the wild, equids encounter many species at waterholes in times of seasonal drought. Therefore, we hypothesized that EHV-1 is stable in water and that water may act as a vector for EHV-1. In order to establish the conditions promoting or hindering EHV-1 longevity, infectivity and genomic stability in water; we exposed EHV-1 to varied water environments (pH, salinity, temperature, and turbidity) in controlled experiments over 21 days. The presence and infectivity of the virus was confirmed by both qPCR and cell culture experiments. Our results show that EHV-1 remains stable and infectious under many conditions in water for up to three weeks.
Fil: Dayaram, Anisha. Leibniz Institute For Zoo And Wildlife Research; Alemania
Fil: Franz, Mathias. Leibniz-institute For Zoo And Wildlife Research; Alemania
Fil: Schattschneider, Alexander. Leibniz-institute For Zoo And Wildlife Research; Alemania
Fil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Freie University Berlin; Alemania
Fil: Bischofberger, Sebastian. Freie University Berlin; Alemania
Fil: Osterrieder, Nikolaus. Freie University Berlin; Alemania
Fil: Greenwood, Alex D.. Leibniz-institute For Zoo And Wildlife Research; Alemania
description For viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. There are many factors including pH, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. Equine herpesvirus type-1 (EHV-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. EHV-1 and recombinants of EHV-1 and EHV-9 are able to cause infections in non-equid animal species, particularly in captive settings. Many of the captive non-equid mammals are not naturally sympatric with equids and do not share enclosures, however, in many cases water sources may overlap. Similarly, in the wild, equids encounter many species at waterholes in times of seasonal drought. Therefore, we hypothesized that EHV-1 is stable in water and that water may act as a vector for EHV-1. In order to establish the conditions promoting or hindering EHV-1 longevity, infectivity and genomic stability in water; we exposed EHV-1 to varied water environments (pH, salinity, temperature, and turbidity) in controlled experiments over 21 days. The presence and infectivity of the virus was confirmed by both qPCR and cell culture experiments. Our results show that EHV-1 remains stable and infectious under many conditions in water for up to three weeks.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49746
Dayaram, Anisha; Franz, Mathias; Schattschneider, Alexander; Damiani, Armando Mario; Bischofberger, Sebastian; et al.; Long term stability and infectivity of herpesviruses in water; Nature Publishing Group; Scientific Reports; 7; 4-2017; 1-10
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49746
identifier_str_mv Dayaram, Anisha; Franz, Mathias; Schattschneider, Alexander; Damiani, Armando Mario; Bischofberger, Sebastian; et al.; Long term stability and infectivity of herpesviruses in water; Nature Publishing Group; Scientific Reports; 7; 4-2017; 1-10
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/srep46559
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep46559
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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