Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)

Autores
Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.
Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
DNA extraction
feces
DNA typing
Primates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152403

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spelling Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)Oklander, Luciana InésMarino, Miguel EduardoZunino, Gabriel EduardoCorach, DanielDNA extractionfecesDNA typingPrimateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioOne2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152403Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-631413-4705CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:59Zoai:ri.conicet.gov.ar:11336/152403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:59.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
title Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
spellingShingle Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
Oklander, Luciana Inés
DNA extraction
feces
DNA typing
Primates
title_short Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
title_full Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
title_fullStr Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
title_full_unstemmed Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
title_sort Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
dc.creator.none.fl_str_mv Oklander, Luciana Inés
Marino, Miguel Eduardo
Zunino, Gabriel Eduardo
Corach, Daniel
author Oklander, Luciana Inés
author_facet Oklander, Luciana Inés
Marino, Miguel Eduardo
Zunino, Gabriel Eduardo
Corach, Daniel
author_role author
author2 Marino, Miguel Eduardo
Zunino, Gabriel Eduardo
Corach, Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv DNA extraction
feces
DNA typing
Primates
topic DNA extraction
feces
DNA typing
Primates
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.
Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.
publishDate 2004
dc.date.none.fl_str_mv 2004-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152403
Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-63
1413-4705
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152403
identifier_str_mv Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-63
1413-4705
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioOne
publisher.none.fl_str_mv BioOne
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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