Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)
- Autores
- Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.
Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
DNA extraction
feces
DNA typing
Primates - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/152403
Ver los metadatos del registro completo
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Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya)Oklander, Luciana InésMarino, Miguel EduardoZunino, Gabriel EduardoCorach, DanielDNA extractionfecesDNA typingPrimateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines.Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioOne2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152403Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-631413-4705CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:59Zoai:ri.conicet.gov.ar:11336/152403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:59.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
title |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
spellingShingle |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) Oklander, Luciana Inés DNA extraction feces DNA typing Primates |
title_short |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
title_full |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
title_fullStr |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
title_full_unstemmed |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
title_sort |
Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya) |
dc.creator.none.fl_str_mv |
Oklander, Luciana Inés Marino, Miguel Eduardo Zunino, Gabriel Eduardo Corach, Daniel |
author |
Oklander, Luciana Inés |
author_facet |
Oklander, Luciana Inés Marino, Miguel Eduardo Zunino, Gabriel Eduardo Corach, Daniel |
author_role |
author |
author2 |
Marino, Miguel Eduardo Zunino, Gabriel Eduardo Corach, Daniel |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
DNA extraction feces DNA typing Primates |
topic |
DNA extraction feces DNA typing Primates |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines. Fil: Oklander, Luciana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina Fil: Marino, Miguel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zunino, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Techniques of molecular genetics are increasingly used tostudy various aspects of the social systems of human andwild non-human primates (Altmann et al., 1996; Gagneuxet al., 1999; Nievergelt et al., 2000; Paabo, 2003;Di Fiore, 2003). In the past, studies of primate moleculargenetics were limited by the availability of blood ortissue samples for DNA extraction. Today, samples such ashair and feces, obtainable through non-invasive methods,are preferred for genetic analysis. This strategy avoids thecapture of the animals, minimizing any undesirable impact ontheir behaviour as well as preventing injuries and infectiousdiseases either to the animal or the sample collector (Constableet al., 2001; Sibal and Samson, 2001). As a result, it is becomingsafer and easier to obtain information on kinship, sexratio, effective population size and gene flow in undisturbedpopulations of arboreal and threatened species.Although a number of recent studies have used non-invasivesampling to examine aspects of the social structure of severalOld World primates (Gagneux et al., 1999; Gerloff et al.,1999; Goossens et al., 2000; Constable et al., 1995, 2001;Vigilant, 2002), only a handful of studies have used feces fromNew World monkeys as a source of DNA for molecular studies(Surridge et al., 2002; Escobar-Paramo, 2000; Böhle andZischler, 2002). The main goal of this study was to test alternativemethods for preserving and subsequently extractingDNA from fecal samples of a New World primate, in order toidentify a low-cost solution that might be broadly applied inmolecular ecological research on platyrrhines. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/152403 Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-63 1413-4705 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/152403 |
identifier_str_mv |
Oklander, Luciana Inés; Marino, Miguel Eduardo; Zunino, Gabriel Eduardo; Corach, Daniel; Preservation and Extration of DNA from Feces in Howler Monkeys (Aluatta caraya); BioOne; Neotropical Primates; 12; 8-2004; 59-63 1413-4705 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioOne |
publisher.none.fl_str_mv |
BioOne |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614524774121472 |
score |
13.070432 |