A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage

Autores
Enrique, Ramón Atanasio; Budeguer, Florencia; Noguera, Aldo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Obtaining transgenic sugarcane lines in vitro is a laborious, expensive, and time-consuming process. To minimize these disadvantages, the presence of transgenes must be checked as soon as possible. Here, we describe a method for direct PCR amplification of DNA from sugarcane plantlets, using smaller quantities of vegetal material, low-cost lab reagents, and rapid processing. By using this methodology, we were able to identify 100% of the transgenic lines generated. The outlined protocol enabled us to perform an earlier checking of the transgene insertions on genomes of transformed lines reducing costs and labor at in vitro stages.
Fil: Enrique, Ramón Atanasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Budeguer, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Noguera, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Materia
SUGARCANE
PSEUDO-COLONY PCR
TRANSGENES INSERTIONS
IN VITRO CULTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261955
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/261955
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stageEnrique, Ramón AtanasioBudeguer, FlorenciaNoguera, AldoSUGARCANEPSEUDO-COLONY PCRTRANSGENES INSERTIONSIN VITRO CULTUREhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Obtaining transgenic sugarcane lines in vitro is a laborious, expensive, and time-consuming process. To minimize these disadvantages, the presence of transgenes must be checked as soon as possible. Here, we describe a method for direct PCR amplification of DNA from sugarcane plantlets, using smaller quantities of vegetal material, low-cost lab reagents, and rapid processing. By using this methodology, we were able to identify 100% of the transgenic lines generated. The outlined protocol enabled us to perform an earlier checking of the transgene insertions on genomes of transformed lines reducing costs and labor at in vitro stages.Fil: Enrique, Ramón Atanasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Budeguer, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Noguera, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaBrazilian Soc Plant Breeding2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261955Enrique, Ramón Atanasio; Budeguer, Florencia; Noguera, Aldo; A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage; Brazilian Soc Plant Breeding; Crop Breeding And Applied Biotechnology; 24; 3; 6-2024; 1-41518-78531984-7033CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/cbab/a/3MkGZHsttVb7rX7WFXZmFNc/info:eu-repo/semantics/altIdentifier/doi/10.1590/1984-70332024v24n3n34info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:03Zoai:ri.conicet.gov.ar:11336/261955instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:03.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
title A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
spellingShingle A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
Enrique, Ramón Atanasio
SUGARCANE
PSEUDO-COLONY PCR
TRANSGENES INSERTIONS
IN VITRO CULTURE
title_short A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
title_full A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
title_fullStr A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
title_full_unstemmed A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
title_sort A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage
dc.creator.none.fl_str_mv Enrique, Ramón Atanasio
Budeguer, Florencia
Noguera, Aldo
author Enrique, Ramón Atanasio
author_facet Enrique, Ramón Atanasio
Budeguer, Florencia
Noguera, Aldo
author_role author
author2 Budeguer, Florencia
Noguera, Aldo
author2_role author
author
dc.subject.none.fl_str_mv SUGARCANE
PSEUDO-COLONY PCR
TRANSGENES INSERTIONS
IN VITRO CULTURE
topic SUGARCANE
PSEUDO-COLONY PCR
TRANSGENES INSERTIONS
IN VITRO CULTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Obtaining transgenic sugarcane lines in vitro is a laborious, expensive, and time-consuming process. To minimize these disadvantages, the presence of transgenes must be checked as soon as possible. Here, we describe a method for direct PCR amplification of DNA from sugarcane plantlets, using smaller quantities of vegetal material, low-cost lab reagents, and rapid processing. By using this methodology, we were able to identify 100% of the transgenic lines generated. The outlined protocol enabled us to perform an earlier checking of the transgene insertions on genomes of transformed lines reducing costs and labor at in vitro stages.
Fil: Enrique, Ramón Atanasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Budeguer, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Noguera, Aldo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina
description Obtaining transgenic sugarcane lines in vitro is a laborious, expensive, and time-consuming process. To minimize these disadvantages, the presence of transgenes must be checked as soon as possible. Here, we describe a method for direct PCR amplification of DNA from sugarcane plantlets, using smaller quantities of vegetal material, low-cost lab reagents, and rapid processing. By using this methodology, we were able to identify 100% of the transgenic lines generated. The outlined protocol enabled us to perform an earlier checking of the transgene insertions on genomes of transformed lines reducing costs and labor at in vitro stages.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261955
Enrique, Ramón Atanasio; Budeguer, Florencia; Noguera, Aldo; A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage; Brazilian Soc Plant Breeding; Crop Breeding And Applied Biotechnology; 24; 3; 6-2024; 1-4
1518-7853
1984-7033
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261955
identifier_str_mv Enrique, Ramón Atanasio; Budeguer, Florencia; Noguera, Aldo; A pseudo-colony PCR method for rapid identification of transgenic sugarcane lines at in vitro stage; Brazilian Soc Plant Breeding; Crop Breeding And Applied Biotechnology; 24; 3; 6-2024; 1-4
1518-7853
1984-7033
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/cbab/a/3MkGZHsttVb7rX7WFXZmFNc/
info:eu-repo/semantics/altIdentifier/doi/10.1590/1984-70332024v24n3n34
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Soc Plant Breeding
publisher.none.fl_str_mv Brazilian Soc Plant Breeding
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082658183938048
score 13.22299

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