Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat

Autores
Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel; Mason, J. Ian; Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Ortega, Hugo Hector
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of the present study was to characterise the expression and tissue distribution of steroid receptors (oestrogen receptor- and (ER, ER), androgen receptor (AR) and progesterone receptor (PR)) and steroidogenic enzymes (P450 aromatase (P450arom), 3-hydroxysteroid dehydrogenase (3-HSD) and steroidogenic acute regulatory protein (StAR)) in letrozole-induced polycystic ovaries of rats. Changes in serum hormone levels, protein expression in whole ovaries by western blot analysis and protein localisation by immunohistochemistry were determined in female rats treated with the aromatase inhibitor letrozole and compared with controls in proestrous and diestrous rats. Increases in the serum LH, FSH and testosterone concentrations were observed in letrozole-treated rats whereas serum oestradiol and progesterone levels were reduced. Protein expression as analysed by western immunoblot was consistent with the immunohistochemical data. Letrozole treatment induced an increase in the expression of AR, StAR and 3-HSD and a decrease in ER. ER, PR and P450arom showed partial changes in relation to some cycle stages. These results indicate that cystogenesis in this experimental model is characterised by changes in steroid receptors and steroidogenic enzyme expression that may be essential to proper ovarian functioning and are in agreement with similar changes observed in women with PCOS.
Fil: Zurvarra, Francisco Miguel. Universidad Nacional del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mason, J. Ian. Edinburgh Medical School, Centre For Reproductive Biology;
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Materia
-HYDROXYSTEROID DEHYDROGENASE
3Β
AROMATASE
POLYCYSTIC OVARIAN SYNDROME
STAR
STEROID RECEPTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97873
Acceder
id CONICETDig_4ad647a6d2f3cb9e6e008af318fdf16d
oai_identifier_str oai:ri.conicet.gov.ar:11336/97873
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in ratZurvarra, Francisco MiguelSalvetti, Natalia RaquelMason, J. IanVelázquez, Melisa María del LujánAlfaro, Natalia SoledadOrtega, Hugo Hector-HYDROXYSTEROID DEHYDROGENASE3ΒAROMATASEPOLYCYSTIC OVARIAN SYNDROMESTARSTEROID RECEPTORShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective of the present study was to characterise the expression and tissue distribution of steroid receptors (oestrogen receptor- and (ER, ER), androgen receptor (AR) and progesterone receptor (PR)) and steroidogenic enzymes (P450 aromatase (P450arom), 3-hydroxysteroid dehydrogenase (3-HSD) and steroidogenic acute regulatory protein (StAR)) in letrozole-induced polycystic ovaries of rats. Changes in serum hormone levels, protein expression in whole ovaries by western blot analysis and protein localisation by immunohistochemistry were determined in female rats treated with the aromatase inhibitor letrozole and compared with controls in proestrous and diestrous rats. Increases in the serum LH, FSH and testosterone concentrations were observed in letrozole-treated rats whereas serum oestradiol and progesterone levels were reduced. Protein expression as analysed by western immunoblot was consistent with the immunohistochemical data. Letrozole treatment induced an increase in the expression of AR, StAR and 3-HSD and a decrease in ER. ER, PR and P450arom showed partial changes in relation to some cycle stages. These results indicate that cystogenesis in this experimental model is characterised by changes in steroid receptors and steroidogenic enzyme expression that may be essential to proper ovarian functioning and are in agreement with similar changes observed in women with PCOS.Fil: Zurvarra, Francisco Miguel. Universidad Nacional del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mason, J. Ian. Edinburgh Medical School, Centre For Reproductive Biology; Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; ArgentinaFil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaCsiro Publishing2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97873Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel; Mason, J. Ian; Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; et al.; Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat; Csiro Publishing; Reproduction Fertility and Development; 21; 7; 12-2009; 827-8391031-3613CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1071/RD09026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:14:12Zoai:ri.conicet.gov.ar:11336/97873instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:14:12.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
title Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
spellingShingle Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
Zurvarra, Francisco Miguel
-HYDROXYSTEROID DEHYDROGENASE
3Β
AROMATASE
POLYCYSTIC OVARIAN SYNDROME
STAR
STEROID RECEPTORS
title_short Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
title_full Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
title_fullStr Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
title_full_unstemmed Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
title_sort Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
dc.creator.none.fl_str_mv Zurvarra, Francisco Miguel
Salvetti, Natalia Raquel
Mason, J. Ian
Velázquez, Melisa María del Luján
Alfaro, Natalia Soledad
Ortega, Hugo Hector
author Zurvarra, Francisco Miguel
author_facet Zurvarra, Francisco Miguel
Salvetti, Natalia Raquel
Mason, J. Ian
Velázquez, Melisa María del Luján
Alfaro, Natalia Soledad
Ortega, Hugo Hector
author_role author
author2 Salvetti, Natalia Raquel
Mason, J. Ian
Velázquez, Melisa María del Luján
Alfaro, Natalia Soledad
Ortega, Hugo Hector
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv -HYDROXYSTEROID DEHYDROGENASE
3Β
AROMATASE
POLYCYSTIC OVARIAN SYNDROME
STAR
STEROID RECEPTORS
topic -HYDROXYSTEROID DEHYDROGENASE
3Β
AROMATASE
POLYCYSTIC OVARIAN SYNDROME
STAR
STEROID RECEPTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of the present study was to characterise the expression and tissue distribution of steroid receptors (oestrogen receptor- and (ER, ER), androgen receptor (AR) and progesterone receptor (PR)) and steroidogenic enzymes (P450 aromatase (P450arom), 3-hydroxysteroid dehydrogenase (3-HSD) and steroidogenic acute regulatory protein (StAR)) in letrozole-induced polycystic ovaries of rats. Changes in serum hormone levels, protein expression in whole ovaries by western blot analysis and protein localisation by immunohistochemistry were determined in female rats treated with the aromatase inhibitor letrozole and compared with controls in proestrous and diestrous rats. Increases in the serum LH, FSH and testosterone concentrations were observed in letrozole-treated rats whereas serum oestradiol and progesterone levels were reduced. Protein expression as analysed by western immunoblot was consistent with the immunohistochemical data. Letrozole treatment induced an increase in the expression of AR, StAR and 3-HSD and a decrease in ER. ER, PR and P450arom showed partial changes in relation to some cycle stages. These results indicate that cystogenesis in this experimental model is characterised by changes in steroid receptors and steroidogenic enzyme expression that may be essential to proper ovarian functioning and are in agreement with similar changes observed in women with PCOS.
Fil: Zurvarra, Francisco Miguel. Universidad Nacional del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mason, J. Ian. Edinburgh Medical School, Centre For Reproductive Biology;
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
description The objective of the present study was to characterise the expression and tissue distribution of steroid receptors (oestrogen receptor- and (ER, ER), androgen receptor (AR) and progesterone receptor (PR)) and steroidogenic enzymes (P450 aromatase (P450arom), 3-hydroxysteroid dehydrogenase (3-HSD) and steroidogenic acute regulatory protein (StAR)) in letrozole-induced polycystic ovaries of rats. Changes in serum hormone levels, protein expression in whole ovaries by western blot analysis and protein localisation by immunohistochemistry were determined in female rats treated with the aromatase inhibitor letrozole and compared with controls in proestrous and diestrous rats. Increases in the serum LH, FSH and testosterone concentrations were observed in letrozole-treated rats whereas serum oestradiol and progesterone levels were reduced. Protein expression as analysed by western immunoblot was consistent with the immunohistochemical data. Letrozole treatment induced an increase in the expression of AR, StAR and 3-HSD and a decrease in ER. ER, PR and P450arom showed partial changes in relation to some cycle stages. These results indicate that cystogenesis in this experimental model is characterised by changes in steroid receptors and steroidogenic enzyme expression that may be essential to proper ovarian functioning and are in agreement with similar changes observed in women with PCOS.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97873
Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel; Mason, J. Ian; Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; et al.; Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat; Csiro Publishing; Reproduction Fertility and Development; 21; 7; 12-2009; 827-839
1031-3613
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97873
identifier_str_mv Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel; Mason, J. Ian; Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; et al.; Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat; Csiro Publishing; Reproduction Fertility and Development; 21; 7; 12-2009; 827-839
1031-3613
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1071/RD09026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1861214428020604928
score 12.720982

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