Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries

Autores
Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Materia
Polycystic Ovaries
Lectin Binding Pattern
Rats
Glycoconjugates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3430

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network_name_str CONICET Digital (CONICET)
spelling Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovariesBarbeito, Claudio GustavoOrtega, Hugo HectorMatiller, ValentinaGimeno, Eduardo JuanSalvetti, Natalia RaquelPolycystic OvariesLectin Binding PatternRatsGlycoconjugateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaFil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaWiley Blackwell Publishing, Inc2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3430Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-8570936-6768enginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12174info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:10Zoai:ri.conicet.gov.ar:11336/3430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:10.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
title Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
spellingShingle Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
Barbeito, Claudio Gustavo
Polycystic Ovaries
Lectin Binding Pattern
Rats
Glycoconjugates
title_short Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
title_full Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
title_fullStr Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
title_full_unstemmed Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
title_sort Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
dc.creator.none.fl_str_mv Barbeito, Claudio Gustavo
Ortega, Hugo Hector
Matiller, Valentina
Gimeno, Eduardo Juan
Salvetti, Natalia Raquel
author Barbeito, Claudio Gustavo
author_facet Barbeito, Claudio Gustavo
Ortega, Hugo Hector
Matiller, Valentina
Gimeno, Eduardo Juan
Salvetti, Natalia Raquel
author_role author
author2 Ortega, Hugo Hector
Matiller, Valentina
Gimeno, Eduardo Juan
Salvetti, Natalia Raquel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polycystic Ovaries
Lectin Binding Pattern
Rats
Glycoconjugates
topic Polycystic Ovaries
Lectin Binding Pattern
Rats
Glycoconjugates
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
description Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3430
Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-857
0936-6768
url http://hdl.handle.net/11336/3430
identifier_str_mv Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-857
0936-6768
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12174
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12174
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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