Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries
- Autores
- Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina - Materia
-
Polycystic Ovaries
Lectin Binding Pattern
Rats
Glycoconjugates - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/3430
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Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovariesBarbeito, Claudio GustavoOrtega, Hugo HectorMatiller, ValentinaGimeno, Eduardo JuanSalvetti, Natalia RaquelPolycystic OvariesLectin Binding PatternRatsGlycoconjugateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics.Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaFil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; ArgentinaWiley Blackwell Publishing, Inc2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3430Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-8570936-6768enginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12174info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:10Zoai:ri.conicet.gov.ar:11336/3430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:10.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
title |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
spellingShingle |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries Barbeito, Claudio Gustavo Polycystic Ovaries Lectin Binding Pattern Rats Glycoconjugates |
title_short |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
title_full |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
title_fullStr |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
title_full_unstemmed |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
title_sort |
Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries |
dc.creator.none.fl_str_mv |
Barbeito, Claudio Gustavo Ortega, Hugo Hector Matiller, Valentina Gimeno, Eduardo Juan Salvetti, Natalia Raquel |
author |
Barbeito, Claudio Gustavo |
author_facet |
Barbeito, Claudio Gustavo Ortega, Hugo Hector Matiller, Valentina Gimeno, Eduardo Juan Salvetti, Natalia Raquel |
author_role |
author |
author2 |
Ortega, Hugo Hector Matiller, Valentina Gimeno, Eduardo Juan Salvetti, Natalia Raquel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Polycystic Ovaries Lectin Binding Pattern Rats Glycoconjugates |
topic |
Polycystic Ovaries Lectin Binding Pattern Rats Glycoconjugates |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics. Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina Fil: Matiller, Valentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico La Plata; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Investigaciones Histológicas Aplicadas. Cátedra de Biología Celular; Argentina |
description |
Contents: Numerous experimental models in different species have been developed for the study of polycystic ovarian syndrome. In this study, we used a model of induction of polycystic ovaries (PO) in rats by exposure to constant light to study the distribution and variations of glycosylated residues present in the different ovarian structures. Seven biotinylated lectins were used (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) on tissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed an increase in affinity for Con-A in the granulosa and theca interna of growing follicles and cysts in animals with PO in relation to the control group. Follicular cysts showed higher affinity for WGA and RCA-I which growing follicles in the same group, and there was a decrease in affinity for PNA in the cysts in relation to the growth of follicles in both groups. Atretic follicles in both groups showed greater labelling with lectins PNA, SBA and RCA-I in relation to healthy follicles. It could also be noted that the zona pellucida of cystic follicles lost the affinity for the lectin Con-A. There was no staining on follicles in any category with the lectins DBA and UEA-I, although it was staining in the corpus luteum (control group) and in the mesothelium and interstitial glands of both groups with DBA. These observations probably reflect changes in the glycosaminoglycans present in the different ovarian compartments or in the glycosylation of cellular components essential for proper follicular dynamics. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/3430 Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-857 0936-6768 |
url |
http://hdl.handle.net/11336/3430 |
identifier_str_mv |
Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Matiller, Valentina; Gimeno, Eduardo Juan; Salvetti, Natalia Raquel; Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 48; 5; 10-2013; 850-857 0936-6768 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/rda.12174 info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/rda.12174 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083500941246464 |
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13.22299 |