The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility

Autores
Alvarez, Diego Ezequiel; Agaisse, Herve
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.
Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Yale. School of Medicine; Estados Unidos
Fil: Agaisse, Herve. University of Yale. School of Medicine; Estados Unidos
Materia
VACCINIA VIRUS
FORMIN
ACTIN
RAC1
FHOD1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24356

id CONICETDig_4ab86d3c4d5277c72d0690e2dfa36156
oai_identifier_str oai:ri.conicet.gov.ar:11336/24356
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motilityAlvarez, Diego EzequielAgaisse, HerveVACCINIA VIRUSFORMINACTINRAC1FHOD1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Yale. School of Medicine; Estados UnidosFil: Agaisse, Herve. University of Yale. School of Medicine; Estados UnidosRockefeller Univ Press2013-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24356Alvarez, Diego Ezequiel; Agaisse, Herve; The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility; Rockefeller Univ Press; Journal Of Cell Biology; 202; 7; 23-9-2013; 1075-10900021-9525CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/202/7/1075info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201303055info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:46Zoai:ri.conicet.gov.ar:11336/24356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:46.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
title The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
spellingShingle The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
Alvarez, Diego Ezequiel
VACCINIA VIRUS
FORMIN
ACTIN
RAC1
FHOD1
title_short The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
title_full The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
title_fullStr The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
title_full_unstemmed The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
title_sort The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
dc.creator.none.fl_str_mv Alvarez, Diego Ezequiel
Agaisse, Herve
author Alvarez, Diego Ezequiel
author_facet Alvarez, Diego Ezequiel
Agaisse, Herve
author_role author
author2 Agaisse, Herve
author2_role author
dc.subject.none.fl_str_mv VACCINIA VIRUS
FORMIN
ACTIN
RAC1
FHOD1
topic VACCINIA VIRUS
FORMIN
ACTIN
RAC1
FHOD1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.
Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Yale. School of Medicine; Estados Unidos
Fil: Agaisse, Herve. University of Yale. School of Medicine; Estados Unidos
description Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24356
Alvarez, Diego Ezequiel; Agaisse, Herve; The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility; Rockefeller Univ Press; Journal Of Cell Biology; 202; 7; 23-9-2013; 1075-1090
0021-9525
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24356
identifier_str_mv Alvarez, Diego Ezequiel; Agaisse, Herve; The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility; Rockefeller Univ Press; Journal Of Cell Biology; 202; 7; 23-9-2013; 1075-1090
0021-9525
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/202/7/1075
info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201303055
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller Univ Press
publisher.none.fl_str_mv Rockefeller Univ Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613290432397312
score 13.070432