Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography

Autores
Lucero, Diego Martín; Zago, Valeria; López, Gustavo; Cacciagiú, Leonardo D.; Lopez, Graciela Ines; Wigdorovitz de Wikinski, Regina Luisa; Nakajima, Katsuyuki; Schreier, Laura Ester
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS). Design and methods: Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d < 1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL. Results: MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90. nm diameter) and large VLDL (60. nm) and slightly lower of typical VLDL (37 nm) (p < 0.04); increased FFA (p. =0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r. =0.58; p. =0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r. = - 0.40; p. =0.04). Conclusion: MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile. © 2012 Elsevier Inc.
Fil: Lucero, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Zago, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: López, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Cacciagiú, Leonardo D.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Lopez, Graciela Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Wigdorovitz de Wikinski, Regina Luisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Nakajima, Katsuyuki. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Gunma University; Japón
Fil: Schreier, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Materia
Adiponectin
Free Fatty Acids
Metabolic Syndrome
Size Exclusion Hplc
Triglyceride Rich Lipoproteins
Vldl
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67279
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67279
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatographyLucero, Diego MartínZago, ValeriaLópez, GustavoCacciagiú, Leonardo D.Lopez, Graciela InesWigdorovitz de Wikinski, Regina LuisaNakajima, KatsuyukiSchreier, Laura EsterAdiponectinFree Fatty AcidsMetabolic SyndromeSize Exclusion HplcTriglyceride Rich LipoproteinsVldlhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS). Design and methods: Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d < 1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL. Results: MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90. nm diameter) and large VLDL (60. nm) and slightly lower of typical VLDL (37 nm) (p < 0.04); increased FFA (p. =0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r. =0.58; p. =0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r. = - 0.40; p. =0.04). Conclusion: MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile. © 2012 Elsevier Inc.Fil: Lucero, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Zago, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: López, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Cacciagiú, Leonardo D.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Lopez, Graciela Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Wigdorovitz de Wikinski, Regina Luisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Nakajima, Katsuyuki. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Gunma University; JapónFil: Schreier, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaPergamon-Elsevier Science Ltd2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67279Lucero, Diego Martín; Zago, Valeria; López, Gustavo; Cacciagiú, Leonardo D.; Lopez, Graciela Ines; et al.; Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography; Pergamon-Elsevier Science Ltd; Clinical Biochemistry; 45; 4-5; 3-2012; 293-2970009-9120CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009912011027780info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinbiochem.2011.12.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:57Zoai:ri.conicet.gov.ar:11336/67279instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:57.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
title Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
spellingShingle Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
Lucero, Diego Martín
Adiponectin
Free Fatty Acids
Metabolic Syndrome
Size Exclusion Hplc
Triglyceride Rich Lipoproteins
Vldl
title_short Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
title_full Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
title_fullStr Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
title_full_unstemmed Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
title_sort Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography
dc.creator.none.fl_str_mv Lucero, Diego Martín
Zago, Valeria
López, Gustavo
Cacciagiú, Leonardo D.
Lopez, Graciela Ines
Wigdorovitz de Wikinski, Regina Luisa
Nakajima, Katsuyuki
Schreier, Laura Ester
author Lucero, Diego Martín
author_facet Lucero, Diego Martín
Zago, Valeria
López, Gustavo
Cacciagiú, Leonardo D.
Lopez, Graciela Ines
Wigdorovitz de Wikinski, Regina Luisa
Nakajima, Katsuyuki
Schreier, Laura Ester
author_role author
author2 Zago, Valeria
López, Gustavo
Cacciagiú, Leonardo D.
Lopez, Graciela Ines
Wigdorovitz de Wikinski, Regina Luisa
Nakajima, Katsuyuki
Schreier, Laura Ester
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adiponectin
Free Fatty Acids
Metabolic Syndrome
Size Exclusion Hplc
Triglyceride Rich Lipoproteins
Vldl
topic Adiponectin
Free Fatty Acids
Metabolic Syndrome
Size Exclusion Hplc
Triglyceride Rich Lipoproteins
Vldl
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS). Design and methods: Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d < 1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL. Results: MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90. nm diameter) and large VLDL (60. nm) and slightly lower of typical VLDL (37 nm) (p < 0.04); increased FFA (p. =0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r. =0.58; p. =0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r. = - 0.40; p. =0.04). Conclusion: MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile. © 2012 Elsevier Inc.
Fil: Lucero, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Zago, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: López, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Cacciagiú, Leonardo D.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Lopez, Graciela Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Wigdorovitz de Wikinski, Regina Luisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Nakajima, Katsuyuki. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Gunma University; Japón
Fil: Schreier, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
description Objective: To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS). Design and methods: Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d < 1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL. Results: MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90. nm diameter) and large VLDL (60. nm) and slightly lower of typical VLDL (37 nm) (p < 0.04); increased FFA (p. =0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r. =0.58; p. =0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r. = - 0.40; p. =0.04). Conclusion: MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile. © 2012 Elsevier Inc.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67279
Lucero, Diego Martín; Zago, Valeria; López, Gustavo; Cacciagiú, Leonardo D.; Lopez, Graciela Ines; et al.; Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography; Pergamon-Elsevier Science Ltd; Clinical Biochemistry; 45; 4-5; 3-2012; 293-297
0009-9120
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67279
identifier_str_mv Lucero, Diego Martín; Zago, Valeria; López, Gustavo; Cacciagiú, Leonardo D.; Lopez, Graciela Ines; et al.; Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography; Pergamon-Elsevier Science Ltd; Clinical Biochemistry; 45; 4-5; 3-2012; 293-297
0009-9120
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009912011027780
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.clinbiochem.2011.12.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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