Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation

Autores
Keller, L. A. M.; Gonzalez Pereyra, Maria Laura; Keller, K. M.; Alonso, Veronica Andrea; Oliveira, A. A.; Almeida, T. X.; Barbosa, T. S.; Nunes, L. M. T.; Cavaglieri, Lilia Reneé; Rosa, C. A. R.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Silage is a widespread practice to preserve forage. Poor storage conditions can lead to mold contamination and mycotoxin production. The aim of this study was to establish the occurrence of toxigenic fungal species and to determine aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 (FB1) and deoxinivalenol (DON) in corn silage intended for bovines before and after fermentation in farms located in São Paulo and Rio de Janeiro States, Brazil. Fungal counts were done by surface-spread method. Toxigenic ability of isolates was evaluated in vitro. AFs natural contamination was determined by TLC and HPLC. Total fungal counts were generally high. Aspergillus flavus, Penicillium citrinum, and Fusarium verticillioides were the prevalent species. Toxigenic strains were isolated. Aflatoxin levels differed (P < 0.0001) from 2 to 45 μg g-1 and from 2 to 100 μg g-1 in pre and post-fermentation samples, respectively. Ochratoxin A, FB1 and DON levels found in pre-fermentation samples were higher than in post-fermentation (P < 0.0001). Mycotoxins and toxigenic fungi were present before and after fermentation in corn silage intended for bovines in Brazil. Aflatoxin B1 (AFB1) increased during storage whereas OTA, FB1 and DON decreased. Determination of mycotoxin levels and their occurrence in corn silage are important to provide information so that the assessments of risk for animal feed and livestock environment could be made.
Fil: Keller, L. A. M.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Keller, K. M.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil
Fil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Oliveira, A. A.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil
Fil: Almeida, T. X.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro;
Fil: Barbosa, T. S.. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Nunes, L. M. T.. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rosa, C. A. R.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Materia
Aflatoxin B1
Corn Silage
Deoxinivalenol
Fumonisin B1
Fungi
Ochratoxin A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentationKeller, L. A. M.Gonzalez Pereyra, Maria LauraKeller, K. M.Alonso, Veronica AndreaOliveira, A. A.Almeida, T. X.Barbosa, T. S.Nunes, L. M. T.Cavaglieri, Lilia ReneéRosa, C. A. R.Aflatoxin B1Corn SilageDeoxinivalenolFumonisin B1FungiOchratoxin Ahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Silage is a widespread practice to preserve forage. Poor storage conditions can lead to mold contamination and mycotoxin production. The aim of this study was to establish the occurrence of toxigenic fungal species and to determine aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 (FB1) and deoxinivalenol (DON) in corn silage intended for bovines before and after fermentation in farms located in São Paulo and Rio de Janeiro States, Brazil. Fungal counts were done by surface-spread method. Toxigenic ability of isolates was evaluated in vitro. AFs natural contamination was determined by TLC and HPLC. Total fungal counts were generally high. Aspergillus flavus, Penicillium citrinum, and Fusarium verticillioides were the prevalent species. Toxigenic strains were isolated. Aflatoxin levels differed (P < 0.0001) from 2 to 45 μg g-1 and from 2 to 100 μg g-1 in pre and post-fermentation samples, respectively. Ochratoxin A, FB1 and DON levels found in pre-fermentation samples were higher than in post-fermentation (P < 0.0001). Mycotoxins and toxigenic fungi were present before and after fermentation in corn silage intended for bovines in Brazil. Aflatoxin B1 (AFB1) increased during storage whereas OTA, FB1 and DON decreased. Determination of mycotoxin levels and their occurrence in corn silage are important to provide information so that the assessments of risk for animal feed and livestock environment could be made.Fil: Keller, L. A. M.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Keller, K. M.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; BrasilFil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Oliveira, A. A.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; BrasilFil: Almeida, T. X.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro;Fil: Barbosa, T. S.. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Nunes, L. M. T.. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Rosa, C. A. R.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; BrasilPergamon-Elsevier Science Ltd2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82002Keller, L. A. M.; Gonzalez Pereyra, Maria Laura; Keller, K. M.; Alonso, Veronica Andrea; Oliveira, A. A.; et al.; Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation; Pergamon-Elsevier Science Ltd; Journal of Stored Products Research; 52; 1-2013; 42-470022-474XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022474X12000732info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jspr.2012.09.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:33Zoai:ri.conicet.gov.ar:11336/82002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:33.739CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
title Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
spellingShingle Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
Keller, L. A. M.
Aflatoxin B1
Corn Silage
Deoxinivalenol
Fumonisin B1
Fungi
Ochratoxin A
title_short Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
title_full Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
title_fullStr Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
title_full_unstemmed Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
title_sort Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation
dc.creator.none.fl_str_mv Keller, L. A. M.
Gonzalez Pereyra, Maria Laura
Keller, K. M.
Alonso, Veronica Andrea
Oliveira, A. A.
Almeida, T. X.
Barbosa, T. S.
Nunes, L. M. T.
Cavaglieri, Lilia Reneé
Rosa, C. A. R.
author Keller, L. A. M.
author_facet Keller, L. A. M.
Gonzalez Pereyra, Maria Laura
Keller, K. M.
Alonso, Veronica Andrea
Oliveira, A. A.
Almeida, T. X.
Barbosa, T. S.
Nunes, L. M. T.
Cavaglieri, Lilia Reneé
Rosa, C. A. R.
author_role author
author2 Gonzalez Pereyra, Maria Laura
Keller, K. M.
Alonso, Veronica Andrea
Oliveira, A. A.
Almeida, T. X.
Barbosa, T. S.
Nunes, L. M. T.
Cavaglieri, Lilia Reneé
Rosa, C. A. R.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aflatoxin B1
Corn Silage
Deoxinivalenol
Fumonisin B1
Fungi
Ochratoxin A
topic Aflatoxin B1
Corn Silage
Deoxinivalenol
Fumonisin B1
Fungi
Ochratoxin A
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Silage is a widespread practice to preserve forage. Poor storage conditions can lead to mold contamination and mycotoxin production. The aim of this study was to establish the occurrence of toxigenic fungal species and to determine aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 (FB1) and deoxinivalenol (DON) in corn silage intended for bovines before and after fermentation in farms located in São Paulo and Rio de Janeiro States, Brazil. Fungal counts were done by surface-spread method. Toxigenic ability of isolates was evaluated in vitro. AFs natural contamination was determined by TLC and HPLC. Total fungal counts were generally high. Aspergillus flavus, Penicillium citrinum, and Fusarium verticillioides were the prevalent species. Toxigenic strains were isolated. Aflatoxin levels differed (P < 0.0001) from 2 to 45 μg g-1 and from 2 to 100 μg g-1 in pre and post-fermentation samples, respectively. Ochratoxin A, FB1 and DON levels found in pre-fermentation samples were higher than in post-fermentation (P < 0.0001). Mycotoxins and toxigenic fungi were present before and after fermentation in corn silage intended for bovines in Brazil. Aflatoxin B1 (AFB1) increased during storage whereas OTA, FB1 and DON decreased. Determination of mycotoxin levels and their occurrence in corn silage are important to provide information so that the assessments of risk for animal feed and livestock environment could be made.
Fil: Keller, L. A. M.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Gonzalez Pereyra, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Keller, K. M.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil
Fil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Oliveira, A. A.. Universidade Federal Rural Do Rio de Janeiro; Brasil. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil
Fil: Almeida, T. X.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro;
Fil: Barbosa, T. S.. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Nunes, L. M. T.. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rosa, C. A. R.. Conselho Nacional de Desenvolvimiento Científico y Tecnológico; Brasil. Universidade Federal Rural Do Rio de Janeiro; Brasil
description Silage is a widespread practice to preserve forage. Poor storage conditions can lead to mold contamination and mycotoxin production. The aim of this study was to establish the occurrence of toxigenic fungal species and to determine aflatoxins (AFs), ochratoxin A (OTA), fumonisin B1 (FB1) and deoxinivalenol (DON) in corn silage intended for bovines before and after fermentation in farms located in São Paulo and Rio de Janeiro States, Brazil. Fungal counts were done by surface-spread method. Toxigenic ability of isolates was evaluated in vitro. AFs natural contamination was determined by TLC and HPLC. Total fungal counts were generally high. Aspergillus flavus, Penicillium citrinum, and Fusarium verticillioides were the prevalent species. Toxigenic strains were isolated. Aflatoxin levels differed (P < 0.0001) from 2 to 45 μg g-1 and from 2 to 100 μg g-1 in pre and post-fermentation samples, respectively. Ochratoxin A, FB1 and DON levels found in pre-fermentation samples were higher than in post-fermentation (P < 0.0001). Mycotoxins and toxigenic fungi were present before and after fermentation in corn silage intended for bovines in Brazil. Aflatoxin B1 (AFB1) increased during storage whereas OTA, FB1 and DON decreased. Determination of mycotoxin levels and their occurrence in corn silage are important to provide information so that the assessments of risk for animal feed and livestock environment could be made.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82002
Keller, L. A. M.; Gonzalez Pereyra, Maria Laura; Keller, K. M.; Alonso, Veronica Andrea; Oliveira, A. A.; et al.; Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation; Pergamon-Elsevier Science Ltd; Journal of Stored Products Research; 52; 1-2013; 42-47
0022-474X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82002
identifier_str_mv Keller, L. A. M.; Gonzalez Pereyra, Maria Laura; Keller, K. M.; Alonso, Veronica Andrea; Oliveira, A. A.; et al.; Fungal and mycotoxins contamination in corn silage: Monitoring risk before and after fermentation; Pergamon-Elsevier Science Ltd; Journal of Stored Products Research; 52; 1-2013; 42-47
0022-474X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022474X12000732
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jspr.2012.09.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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