Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein

Autores
Montanari, Sonia; Wang, Xing-Hua; Yannarelli, Gustavo Gabriel; Dayan, Victor; Berger, Thorsten; Zocche, Larissa; Kobayashi, Eiji; Viswanathan, Sowmya; Keating, Armand
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We generated and characterized a novel inbred Tg Lewis rat strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg rat skin on wild-type rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation
Fil: Montanari, Sonia. University Health Network. Toronto; Canadá. University of Toronto; Canadá. Princess Margaret Cancer Centre. Toronto; Canadá
Fil: Wang, Xing-Hua. University Health Network. Toronto; Canadá
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Health Network. Toronto; Canadá
Fil: Dayan, Victor. University Health Network. Toronto; Canadá
Fil: Berger, Thorsten. University Health Network. Toronto; Canadá
Fil: Zocche, Larissa. University Health Network. Toronto; Canadá
Fil: Kobayashi, Eiji. Jichi Medical School. Tochigi; Japón
Fil: Viswanathan, Sowmya. University Health Network. Toronto; Canadá
Fil: Keating, Armand. University of Toronto; Canadá. University Health Network. Toronto; Canadá
Materia
Mesenchymal Stromal Cells
Dsred Monomeric Protein
Heterotopic Heart Transplantation
Immunogenicity
Skin Grafting
Transgenic Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36511
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36511
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent proteinMontanari, SoniaWang, Xing-HuaYannarelli, Gustavo GabrielDayan, VictorBerger, ThorstenZocche, LarissaKobayashi, EijiViswanathan, SowmyaKeating, ArmandMesenchymal Stromal CellsDsred Monomeric ProteinHeterotopic Heart TransplantationImmunogenicitySkin GraftingTransgenic Rathttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We generated and characterized a novel inbred Tg Lewis rat strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg rat skin on wild-type rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantationFil: Montanari, Sonia. University Health Network. Toronto; Canadá. University of Toronto; Canadá. Princess Margaret Cancer Centre. Toronto; CanadáFil: Wang, Xing-Hua. University Health Network. Toronto; CanadáFil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Health Network. Toronto; CanadáFil: Dayan, Victor. University Health Network. Toronto; CanadáFil: Berger, Thorsten. University Health Network. Toronto; CanadáFil: Zocche, Larissa. University Health Network. Toronto; CanadáFil: Kobayashi, Eiji. Jichi Medical School. Tochigi; JapónFil: Viswanathan, Sowmya. University Health Network. Toronto; CanadáFil: Keating, Armand. University of Toronto; Canadá. University Health Network. Toronto; CanadáSpringer2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36511Montanari, Sonia; Wang, Xing-Hua; Yannarelli, Gustavo Gabriel; Dayan, Victor; Berger, Thorsten; et al.; Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein; Springer; Transgenic Research; 23; 5; 10-2014; 779-7930962-8819CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11248-014-9814-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11248-014-9814-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:44Zoai:ri.conicet.gov.ar:11336/36511instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:44.69CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
title Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
spellingShingle Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
Montanari, Sonia
Mesenchymal Stromal Cells
Dsred Monomeric Protein
Heterotopic Heart Transplantation
Immunogenicity
Skin Grafting
Transgenic Rat
title_short Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
title_full Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
title_fullStr Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
title_full_unstemmed Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
title_sort Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein
dc.creator.none.fl_str_mv Montanari, Sonia
Wang, Xing-Hua
Yannarelli, Gustavo Gabriel
Dayan, Victor
Berger, Thorsten
Zocche, Larissa
Kobayashi, Eiji
Viswanathan, Sowmya
Keating, Armand
author Montanari, Sonia
author_facet Montanari, Sonia
Wang, Xing-Hua
Yannarelli, Gustavo Gabriel
Dayan, Victor
Berger, Thorsten
Zocche, Larissa
Kobayashi, Eiji
Viswanathan, Sowmya
Keating, Armand
author_role author
author2 Wang, Xing-Hua
Yannarelli, Gustavo Gabriel
Dayan, Victor
Berger, Thorsten
Zocche, Larissa
Kobayashi, Eiji
Viswanathan, Sowmya
Keating, Armand
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mesenchymal Stromal Cells
Dsred Monomeric Protein
Heterotopic Heart Transplantation
Immunogenicity
Skin Grafting
Transgenic Rat
topic Mesenchymal Stromal Cells
Dsred Monomeric Protein
Heterotopic Heart Transplantation
Immunogenicity
Skin Grafting
Transgenic Rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We generated and characterized a novel inbred Tg Lewis rat strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg rat skin on wild-type rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation
Fil: Montanari, Sonia. University Health Network. Toronto; Canadá. University of Toronto; Canadá. Princess Margaret Cancer Centre. Toronto; Canadá
Fil: Wang, Xing-Hua. University Health Network. Toronto; Canadá
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Health Network. Toronto; Canadá
Fil: Dayan, Victor. University Health Network. Toronto; Canadá
Fil: Berger, Thorsten. University Health Network. Toronto; Canadá
Fil: Zocche, Larissa. University Health Network. Toronto; Canadá
Fil: Kobayashi, Eiji. Jichi Medical School. Tochigi; Japón
Fil: Viswanathan, Sowmya. University Health Network. Toronto; Canadá
Fil: Keating, Armand. University of Toronto; Canadá. University Health Network. Toronto; Canadá
description The inbred rat is a suitable model for studying human disease and because of its larger size is more amenable to complex surgical manipulation than the mouse. While the rodent fulfills many of the criteria for transplantation research, an important requirement is the ability to mark and track donors cells and assess organ viability. However, tracking ability is limited by the availability of transgenic (Tg) rats that express suitable luminescent or fluorescent proteins. Red fluorescent protein cloned from Discosoma coral (DsRed) has several advantages over other fluorescent proteins, including in vivo detection in the whole animal and ex vivo visualization in organs as there is no interference with autofluorescence. We generated and characterized a novel inbred Tg Lewis rat strain expressing DsRed monomeric (DsRed mono) fluorescent protein under the control of a ubiquitously expressed ROSA26 promoter. DsRed mono Tg rats ubiquitously expressed the marker gene as detected by RT-PCR but the protein was expressed at varying levels in different organs. Conventional skin grafting experiments showed acceptance of DsRed monomeric Tg rat skin on wild-type rats for more than 30 days. Cardiac transplantation of DsRed monomeric Tg rat hearts into wild-type recipients further showed graft acceptance and long-term organ viability (>6 months). The DsRed monomeric Tg rat provides marked cells and/or organs that can be followed for long periods without immune rejection and therefore is a suitable model to investigate cell tracking and organ transplantation
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36511
Montanari, Sonia; Wang, Xing-Hua; Yannarelli, Gustavo Gabriel; Dayan, Victor; Berger, Thorsten; et al.; Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein; Springer; Transgenic Research; 23; 5; 10-2014; 779-793
0962-8819
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36511
identifier_str_mv Montanari, Sonia; Wang, Xing-Hua; Yannarelli, Gustavo Gabriel; Dayan, Victor; Berger, Thorsten; et al.; Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein; Springer; Transgenic Research; 23; 5; 10-2014; 779-793
0962-8819
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11248-014-9814-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11248-014-9814-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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