Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)

Autores
Regensburger, Cristian Daniel; Gos, Maria Laura; Ctibor, J.; Moré, Gastón Andrés
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. Sarcocystis spp. producing macroscopical cysts in these animals have been nominated as S. aucheniae, S. tilopodi, and S. guanicoecanis. The aim of the present study was to characterize morphologically and molecularly Sarcocystis spp. producing macroscopical cysts in guanacos (Lama guanicoe). Methods: Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies. Results: Cyst wall villar protrusions were irregular with a “tree” or “cauliflower-like” shape which measured 3-4.5 µm height by 2.5-3.5 µm width. A consensus 18S rRNA full gene sequence of 1875 bp was obtained and showed the highest identity (≥ 99 %) with sequences of S. aucheniae reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and 18S rRNA gene obtained from guanacos were identical and consistent with the descriptions of S. aucheniae. Conclusion: This study confirms that guanacos are infected by the same Sarcocystis spp. affecting other SAC, named S. aucheniae, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.
Fil: Regensburger, Cristian Daniel. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Ctibor, J.. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; Argentina
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Sarcocystis
Polymerase Chain Reaction
Sequence Analysis
DNA
Microscopy
Electron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55022
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)Regensburger, Cristian DanielGos, Maria LauraCtibor, J.Moré, Gastón AndrésSarcocystisPolymerase Chain ReactionSequence AnalysisDNAMicroscopyElectronhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. Sarcocystis spp. producing macroscopical cysts in these animals have been nominated as S. aucheniae, S. tilopodi, and S. guanicoecanis. The aim of the present study was to characterize morphologically and molecularly Sarcocystis spp. producing macroscopical cysts in guanacos (Lama guanicoe). Methods: Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies. Results: Cyst wall villar protrusions were irregular with a “tree” or “cauliflower-like” shape which measured 3-4.5 µm height by 2.5-3.5 µm width. A consensus 18S rRNA full gene sequence of 1875 bp was obtained and showed the highest identity (≥ 99 %) with sequences of S. aucheniae reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and 18S rRNA gene obtained from guanacos were identical and consistent with the descriptions of S. aucheniae. Conclusion: This study confirms that guanacos are infected by the same Sarcocystis spp. affecting other SAC, named S. aucheniae, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.Fil: Regensburger, Cristian Daniel. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; ArgentinaFil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Ctibor, J.. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; ArgentinaFil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaShahid Sadoughi University of Medical Sciences2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55022Regensburger, Cristian Daniel; Gos, Maria Laura; Ctibor, J.; Moré, Gastón Andrés; Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe); Shahid Sadoughi University of Medical Sciences; Journal of Food Quality and Hazards Control; 2; 4; 12-2015; 118-1212345-685X2345-6825CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-115&slc_lang=fa&sid=1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:07Zoai:ri.conicet.gov.ar:11336/55022instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:07.976CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
title Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
spellingShingle Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
Regensburger, Cristian Daniel
Sarcocystis
Polymerase Chain Reaction
Sequence Analysis
DNA
Microscopy
Electron
title_short Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
title_full Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
title_fullStr Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
title_full_unstemmed Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
title_sort Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe)
dc.creator.none.fl_str_mv Regensburger, Cristian Daniel
Gos, Maria Laura
Ctibor, J.
Moré, Gastón Andrés
author Regensburger, Cristian Daniel
author_facet Regensburger, Cristian Daniel
Gos, Maria Laura
Ctibor, J.
Moré, Gastón Andrés
author_role author
author2 Gos, Maria Laura
Ctibor, J.
Moré, Gastón Andrés
author2_role author
author
author
dc.subject.none.fl_str_mv Sarcocystis
Polymerase Chain Reaction
Sequence Analysis
DNA
Microscopy
Electron
topic Sarcocystis
Polymerase Chain Reaction
Sequence Analysis
DNA
Microscopy
Electron
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. Sarcocystis spp. producing macroscopical cysts in these animals have been nominated as S. aucheniae, S. tilopodi, and S. guanicoecanis. The aim of the present study was to characterize morphologically and molecularly Sarcocystis spp. producing macroscopical cysts in guanacos (Lama guanicoe). Methods: Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies. Results: Cyst wall villar protrusions were irregular with a “tree” or “cauliflower-like” shape which measured 3-4.5 µm height by 2.5-3.5 µm width. A consensus 18S rRNA full gene sequence of 1875 bp was obtained and showed the highest identity (≥ 99 %) with sequences of S. aucheniae reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and 18S rRNA gene obtained from guanacos were identical and consistent with the descriptions of S. aucheniae. Conclusion: This study confirms that guanacos are infected by the same Sarcocystis spp. affecting other SAC, named S. aucheniae, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.
Fil: Regensburger, Cristian Daniel. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; Argentina
Fil: Gos, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Ctibor, J.. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; Argentina
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Background: Sarcocystosis in South American camelids (SAC) is an important parasitic disease which results in economical loss due to carcass condemnation. Meat products from camelids are significant source of animal protein in several American countries. Sarcocystis spp. producing macroscopical cysts in these animals have been nominated as S. aucheniae, S. tilopodi, and S. guanicoecanis. The aim of the present study was to characterize morphologically and molecularly Sarcocystis spp. producing macroscopical cysts in guanacos (Lama guanicoe). Methods: Samples of macroscopical cysts were dissected from guanaco muscles slaughtered in Santa Cruz province, Argentina. The samples were processed by transmission electron microscopy, polymerase chain reaction (PCR), cloning and sequencing studies. Results: Cyst wall villar protrusions were irregular with a “tree” or “cauliflower-like” shape which measured 3-4.5 µm height by 2.5-3.5 µm width. A consensus 18S rRNA full gene sequence of 1875 bp was obtained and showed the highest identity (≥ 99 %) with sequences of S. aucheniae reported in GenBank. The ultra structure of the cyst wall from macroscopical cysts and 18S rRNA gene obtained from guanacos were identical and consistent with the descriptions of S. aucheniae. Conclusion: This study confirms that guanacos are infected by the same Sarcocystis spp. affecting other SAC, named S. aucheniae, and reinforce the suggestion that nomination of new species should contain morphological and molecular data to reach validity. In future, this data could be useful to obtain proper identifications in post-mortem inspection and helpful to improve meat safety.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55022
Regensburger, Cristian Daniel; Gos, Maria Laura; Ctibor, J.; Moré, Gastón Andrés; Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe); Shahid Sadoughi University of Medical Sciences; Journal of Food Quality and Hazards Control; 2; 4; 12-2015; 118-121
2345-685X
2345-6825
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55022
identifier_str_mv Regensburger, Cristian Daniel; Gos, Maria Laura; Ctibor, J.; Moré, Gastón Andrés; Morphological and Molecular Characteristics of Sarcocystis aucheniae Isolated from Meat of Guanaco (Lama guanicoe); Shahid Sadoughi University of Medical Sciences; Journal of Food Quality and Hazards Control; 2; 4; 12-2015; 118-121
2345-685X
2345-6825
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-1-115&slc_lang=fa&sid=1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Shahid Sadoughi University of Medical Sciences
publisher.none.fl_str_mv Shahid Sadoughi University of Medical Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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