Alternation between dietary protein depletion and normal feeding cause liver damage in mouse

Autores
Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Giudici, Ana Marcela; Crupkin, Andrea Carina; Barbeito, Claudio Gustavo; Ronchi, Virginia Paola; Chisari, Andrea Nancy; Conde, Ruben Danilo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions.
Fil: Caballero, Veronica Jorgelina. Universidad Nacional de Mar del Plata; Argentina
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Giudici, Ana Marcela. Universidad Nacional de Mar del Plata; Argentina
Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina
Fil: Ronchi, Virginia Paola. Universidad Nacional de Mar del Plata; Argentina
Fil: Chisari, Andrea Nancy. Universidad Nacional de Mar del Plata; Argentina
Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
CYCLES OF PROTEIN DEPLETION
LIVER DAMAGE
MOUSE LIVER
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/126494
Acceder
id CONICETDig_489a8bd6787a9eb5ead9713deca6ca65
oai_identifier_str oai:ri.conicet.gov.ar:11336/126494
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alternation between dietary protein depletion and normal feeding cause liver damage in mouseCaballero, Veronica JorgelinaMendieta, Julieta ReneeGiudici, Ana MarcelaCrupkin, Andrea CarinaBarbeito, Claudio GustavoRonchi, Virginia PaolaChisari, Andrea NancyConde, Ruben DaniloCYCLES OF PROTEIN DEPLETIONLIVER DAMAGEMOUSE LIVEROXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions.Fil: Caballero, Veronica Jorgelina. Universidad Nacional de Mar del Plata; ArgentinaFil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Giudici, Ana Marcela. Universidad Nacional de Mar del Plata; ArgentinaFil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; ArgentinaFil: Ronchi, Virginia Paola. Universidad Nacional de Mar del Plata; ArgentinaFil: Chisari, Andrea Nancy. Universidad Nacional de Mar del Plata; ArgentinaFil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaServicio Publicaciones Universidad Navarra2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/126494Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Giudici, Ana Marcela; Crupkin, Andrea Carina; Barbeito, Claudio Gustavo; et al.; Alternation between dietary protein depletion and normal feeding cause liver damage in mouse; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 67; 1; 3-2011; 43-521138-7548CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13105-010-0047-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13105-010-0047-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:04Zoai:ri.conicet.gov.ar:11336/126494instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:04.628CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
spellingShingle Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
Caballero, Veronica Jorgelina
CYCLES OF PROTEIN DEPLETION
LIVER DAMAGE
MOUSE LIVER
OXIDATIVE STRESS
title_short Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_full Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_fullStr Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_full_unstemmed Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
title_sort Alternation between dietary protein depletion and normal feeding cause liver damage in mouse
dc.creator.none.fl_str_mv Caballero, Veronica Jorgelina
Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea Nancy
Conde, Ruben Danilo
author Caballero, Veronica Jorgelina
author_facet Caballero, Veronica Jorgelina
Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea Nancy
Conde, Ruben Danilo
author_role author
author2 Mendieta, Julieta Renee
Giudici, Ana Marcela
Crupkin, Andrea Carina
Barbeito, Claudio Gustavo
Ronchi, Virginia Paola
Chisari, Andrea Nancy
Conde, Ruben Danilo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CYCLES OF PROTEIN DEPLETION
LIVER DAMAGE
MOUSE LIVER
OXIDATIVE STRESS
topic CYCLES OF PROTEIN DEPLETION
LIVER DAMAGE
MOUSE LIVER
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions.
Fil: Caballero, Veronica Jorgelina. Universidad Nacional de Mar del Plata; Argentina
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Giudici, Ana Marcela. Universidad Nacional de Mar del Plata; Argentina
Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina
Fil: Ronchi, Virginia Paola. Universidad Nacional de Mar del Plata; Argentina
Fil: Chisari, Andrea Nancy. Universidad Nacional de Mar del Plata; Argentina
Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The effect of frequent protein malnutrition on liver function has not been intensively examined. Thus, the effects of alternating 5 days of a protein and amino acid-free diet followed by 5 days of a complete diet repeated three times (3 PFD-CD) on female mouse liver were examined. The expression of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) in liver were assessed by proteomics, reverse transcriptase-polymerase chain reaction and Northern blotting. The activities of liver GSTs, glutathione reductase (GR) and catalase (CAT), as well as serum glutamic-oxaloacetic transaminase (SGOT) and glutamic-pyruvic transaminase (SGPT) were also tested. Additionally, oxidative damage was examined by measuring of protein carbonylation and lipid peroxidation. Liver histology was examined by light and electron microscopy. Compared with control mice, 3 PFD-CD increased the content of FAS protein (+90%) and FAS mRNA (+30%), while the levels of CAIII and CAIII mRNAs were decreased (-48% and -64%, respectively). In addition, 3 PFD-CD did not significantly change the content of GSTP1 but produced an increase in its mRNA level (+20%), while it decreased the activities of both CAT (-66%) and GSTs (-26%). After 3 PFD-CD, liver protein carbonylation and lipid peroxidation were increased by +55% and +95%, respectively. In serum, 3 PFD-CD increased the activities of both SGOT (+30%) and SGPT (+61%). In addition, 3 PFD-CD showed a histological pattern characteristic of hepatic damage. All together, these data suggest that frequent dietary amino acid deprivation causes hepatic metabolic and ultrastructural changes in a fashion similar to precancerous or cancerous conditions.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/126494
Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Giudici, Ana Marcela; Crupkin, Andrea Carina; Barbeito, Claudio Gustavo; et al.; Alternation between dietary protein depletion and normal feeding cause liver damage in mouse; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 67; 1; 3-2011; 43-52
1138-7548
CONICET Digital
CONICET
url http://hdl.handle.net/11336/126494
identifier_str_mv Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Giudici, Ana Marcela; Crupkin, Andrea Carina; Barbeito, Claudio Gustavo; et al.; Alternation between dietary protein depletion and normal feeding cause liver damage in mouse; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 67; 1; 3-2011; 43-52
1138-7548
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13105-010-0047-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s13105-010-0047-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Servicio Publicaciones Universidad Navarra
publisher.none.fl_str_mv Servicio Publicaciones Universidad Navarra
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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