Brain somatostatin receptors are up-regulated in somatostatin-deficient mice

Autores
Ramírez, José L.; Mouchantaf, Rania; Kumar, Ujendra; Otero Corchon, Veronica; Rubinstein, Marcelo; Low, Malcolm J.; Patel, Yogesh C.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The peptide somatostatin (SST) is widely synthesized in the brain and periphery and acts through a family of five receptors (SSTR1-5) to exert numerous effects. A gene product related to SST, cortistatin (CST), also interacts with SSTR1-5. Here we have investigated the regulation of SSTR1-5 and of CST in SST knockout (SSTKO) mice. The five SSTRs were quantitated individually by subtype-selective binding analysis, by immunocytochemistry, and by mRNA measurement and showed, in the brain of SSTKO mice, up-regulation of subtypes 1, 2, 4, and 5, and down-regulation of SSTR3. Peripheral tissues displayed both subtype- and tissue-specific changes in SSTR1-5 mRNA levels of expression. Lack of SST did not up-regulate normal CST expression in brain nor did it induce its expression in the periphery. SST-like immunoreactivity, however, was induced in the proximal midgut in SSTKO animals, suggesting intestinal expression of a novel SST-like gene.
Fil: Ramírez, José L.. McGill University; Canadá
Fil: Mouchantaf, Rania. McGill University; Canadá
Fil: Kumar, Ujendra. McGill University; Canadá
Fil: Otero Corchon, Veronica. Oregon Health and Science University; Estados Unidos
Fil: Rubinstein, Marcelo. Oregon Health and Science University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Patel, Yogesh C.. McGill University; Canadá
Materia
Somatostatina
Ratón Knockout
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79786
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79786
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brain somatostatin receptors are up-regulated in somatostatin-deficient miceRamírez, José L.Mouchantaf, RaniaKumar, UjendraOtero Corchon, VeronicaRubinstein, MarceloLow, Malcolm J.Patel, Yogesh C.SomatostatinaRatón Knockouthttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The peptide somatostatin (SST) is widely synthesized in the brain and periphery and acts through a family of five receptors (SSTR1-5) to exert numerous effects. A gene product related to SST, cortistatin (CST), also interacts with SSTR1-5. Here we have investigated the regulation of SSTR1-5 and of CST in SST knockout (SSTKO) mice. The five SSTRs were quantitated individually by subtype-selective binding analysis, by immunocytochemistry, and by mRNA measurement and showed, in the brain of SSTKO mice, up-regulation of subtypes 1, 2, 4, and 5, and down-regulation of SSTR3. Peripheral tissues displayed both subtype- and tissue-specific changes in SSTR1-5 mRNA levels of expression. Lack of SST did not up-regulate normal CST expression in brain nor did it induce its expression in the periphery. SST-like immunoreactivity, however, was induced in the proximal midgut in SSTKO animals, suggesting intestinal expression of a novel SST-like gene.Fil: Ramírez, José L.. McGill University; CanadáFil: Mouchantaf, Rania. McGill University; CanadáFil: Kumar, Ujendra. McGill University; CanadáFil: Otero Corchon, Veronica. Oregon Health and Science University; Estados UnidosFil: Rubinstein, Marcelo. Oregon Health and Science University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Low, Malcolm J.. Oregon Health and Science University; Estados UnidosFil: Patel, Yogesh C.. McGill University; CanadáEndocrine Society2002-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79786Ramírez, José L.; Mouchantaf, Rania; Kumar, Ujendra; Otero Corchon, Veronica; Rubinstein, Marcelo; et al.; Brain somatostatin receptors are up-regulated in somatostatin-deficient mice; Endocrine Society; Molecular Endocrinology; 16; 8; 8-2002; 1951-19630888-8809CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12145348info:eu-repo/semantics/altIdentifier/doi/10.1210/me.2002-0068info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mend/article/16/8/1951/2741841info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:54Zoai:ri.conicet.gov.ar:11336/79786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:54.584CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
title Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
spellingShingle Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
Ramírez, José L.
Somatostatina
Ratón Knockout
title_short Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
title_full Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
title_fullStr Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
title_full_unstemmed Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
title_sort Brain somatostatin receptors are up-regulated in somatostatin-deficient mice
dc.creator.none.fl_str_mv Ramírez, José L.
Mouchantaf, Rania
Kumar, Ujendra
Otero Corchon, Veronica
Rubinstein, Marcelo
Low, Malcolm J.
Patel, Yogesh C.
author Ramírez, José L.
author_facet Ramírez, José L.
Mouchantaf, Rania
Kumar, Ujendra
Otero Corchon, Veronica
Rubinstein, Marcelo
Low, Malcolm J.
Patel, Yogesh C.
author_role author
author2 Mouchantaf, Rania
Kumar, Ujendra
Otero Corchon, Veronica
Rubinstein, Marcelo
Low, Malcolm J.
Patel, Yogesh C.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Somatostatina
Ratón Knockout
topic Somatostatina
Ratón Knockout
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The peptide somatostatin (SST) is widely synthesized in the brain and periphery and acts through a family of five receptors (SSTR1-5) to exert numerous effects. A gene product related to SST, cortistatin (CST), also interacts with SSTR1-5. Here we have investigated the regulation of SSTR1-5 and of CST in SST knockout (SSTKO) mice. The five SSTRs were quantitated individually by subtype-selective binding analysis, by immunocytochemistry, and by mRNA measurement and showed, in the brain of SSTKO mice, up-regulation of subtypes 1, 2, 4, and 5, and down-regulation of SSTR3. Peripheral tissues displayed both subtype- and tissue-specific changes in SSTR1-5 mRNA levels of expression. Lack of SST did not up-regulate normal CST expression in brain nor did it induce its expression in the periphery. SST-like immunoreactivity, however, was induced in the proximal midgut in SSTKO animals, suggesting intestinal expression of a novel SST-like gene.
Fil: Ramírez, José L.. McGill University; Canadá
Fil: Mouchantaf, Rania. McGill University; Canadá
Fil: Kumar, Ujendra. McGill University; Canadá
Fil: Otero Corchon, Veronica. Oregon Health and Science University; Estados Unidos
Fil: Rubinstein, Marcelo. Oregon Health and Science University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Patel, Yogesh C.. McGill University; Canadá
description The peptide somatostatin (SST) is widely synthesized in the brain and periphery and acts through a family of five receptors (SSTR1-5) to exert numerous effects. A gene product related to SST, cortistatin (CST), also interacts with SSTR1-5. Here we have investigated the regulation of SSTR1-5 and of CST in SST knockout (SSTKO) mice. The five SSTRs were quantitated individually by subtype-selective binding analysis, by immunocytochemistry, and by mRNA measurement and showed, in the brain of SSTKO mice, up-regulation of subtypes 1, 2, 4, and 5, and down-regulation of SSTR3. Peripheral tissues displayed both subtype- and tissue-specific changes in SSTR1-5 mRNA levels of expression. Lack of SST did not up-regulate normal CST expression in brain nor did it induce its expression in the periphery. SST-like immunoreactivity, however, was induced in the proximal midgut in SSTKO animals, suggesting intestinal expression of a novel SST-like gene.
publishDate 2002
dc.date.none.fl_str_mv 2002-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79786
Ramírez, José L.; Mouchantaf, Rania; Kumar, Ujendra; Otero Corchon, Veronica; Rubinstein, Marcelo; et al.; Brain somatostatin receptors are up-regulated in somatostatin-deficient mice; Endocrine Society; Molecular Endocrinology; 16; 8; 8-2002; 1951-1963
0888-8809
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79786
identifier_str_mv Ramírez, José L.; Mouchantaf, Rania; Kumar, Ujendra; Otero Corchon, Veronica; Rubinstein, Marcelo; et al.; Brain somatostatin receptors are up-regulated in somatostatin-deficient mice; Endocrine Society; Molecular Endocrinology; 16; 8; 8-2002; 1951-1963
0888-8809
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12145348
info:eu-repo/semantics/altIdentifier/doi/10.1210/me.2002-0068
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mend/article/16/8/1951/2741841
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269429285519360
score 13.13397

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