Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy
- Autores
- Wenz, Jorge Javier
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- By exploiting the sensitivity of the NIR spectrum to hydrogen bonds, particularly in the first overtone of water around 1450/55 nm, the influence of temperature and phospholipid head group on the H-bond network and water polymerization in membranes of DMPA and DMPC was studied. Principal components analysis of the spectra was used to disclose subtle spectral changes that mirror alterations in the vibrational energy of the water O-H bonds, as a measure of the H-bond network. Increasing temperatures drastically diminished the number of strongly H-bonded water molecules and increase the number of weakly H-bonded waters. This dominating effect of temperature was missing after the subtraction of the pure water spectra from the lipid-containing ones, suggesting the presence of two water populations. An intriguing secondary effect of temperature was also revealed. At the water:lipid interface, phospholipids exhibited an effect qualitatively similar to that of the temperature. DMPA, and particularly DMPC, disrupted the H-bond network in the neighboring water, reducing water polymerization and strengthening the water O-H bonds. The type of the polar head affects the H-bonds more than duplicate the concentration of the lipid from 250 to 500 μM. A relation between head group structure and the effect on the H-bonds network and the existence of two populations of water molecules are discussed.
Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina De Investigación Clínica
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina De Inmunología
Sociedad Argentina De Andrología
Sociedad Argentina De Biofísica
Sociedad Argentina De Biología
Sociedad Argentina De Farmacología Experimental
Sociedad Argentina De Fisiología
Sociedad Argentina De Hematología
Sociedad Argentina De Protozoología - Materia
-
MEMBRANES
WATER
NEAR INFRARED SPECTROSCOPY
CHEMOMETRICS
PRINCIPAL COMPONENT ANALYSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/244021
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Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopyWenz, Jorge JavierMEMBRANESWATERNEAR INFRARED SPECTROSCOPYCHEMOMETRICSPRINCIPAL COMPONENT ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1By exploiting the sensitivity of the NIR spectrum to hydrogen bonds, particularly in the first overtone of water around 1450/55 nm, the influence of temperature and phospholipid head group on the H-bond network and water polymerization in membranes of DMPA and DMPC was studied. Principal components analysis of the spectra was used to disclose subtle spectral changes that mirror alterations in the vibrational energy of the water O-H bonds, as a measure of the H-bond network. Increasing temperatures drastically diminished the number of strongly H-bonded water molecules and increase the number of weakly H-bonded waters. This dominating effect of temperature was missing after the subtraction of the pure water spectra from the lipid-containing ones, suggesting the presence of two water populations. An intriguing secondary effect of temperature was also revealed. At the water:lipid interface, phospholipids exhibited an effect qualitatively similar to that of the temperature. DMPA, and particularly DMPC, disrupted the H-bond network in the neighboring water, reducing water polymerization and strengthening the water O-H bonds. The type of the polar head affects the H-bonds more than duplicate the concentration of the lipid from 250 to 500 μM. A relation between head group structure and the effect on the H-bonds network and the existence of two populations of water molecules are discussed.Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de ProtozoologíaBuenos AiresArgentinaSociedad Argentina De Investigación ClínicaSociedad Argentina De Investigación Bioquímica Y Biología MolecularSociedad Argentina De InmunologíaSociedad Argentina De AndrologíaSociedad Argentina De BiofísicaSociedad Argentina De BiologíaSociedad Argentina De Farmacología ExperimentalSociedad Argentina De FisiologíaSociedad Argentina De HematologíaSociedad Argentina De ProtozoologíaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244021Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 412-4120076-60461669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol77-17/Vol.77SuplementoI-2017.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:24Zoai:ri.conicet.gov.ar:11336/244021instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:24.627CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
title |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
spellingShingle |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy Wenz, Jorge Javier MEMBRANES WATER NEAR INFRARED SPECTROSCOPY CHEMOMETRICS PRINCIPAL COMPONENT ANALYSIS |
title_short |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
title_full |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
title_fullStr |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
title_full_unstemmed |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
title_sort |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy |
dc.creator.none.fl_str_mv |
Wenz, Jorge Javier |
author |
Wenz, Jorge Javier |
author_facet |
Wenz, Jorge Javier |
author_role |
author |
dc.subject.none.fl_str_mv |
MEMBRANES WATER NEAR INFRARED SPECTROSCOPY CHEMOMETRICS PRINCIPAL COMPONENT ANALYSIS |
topic |
MEMBRANES WATER NEAR INFRARED SPECTROSCOPY CHEMOMETRICS PRINCIPAL COMPONENT ANALYSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
By exploiting the sensitivity of the NIR spectrum to hydrogen bonds, particularly in the first overtone of water around 1450/55 nm, the influence of temperature and phospholipid head group on the H-bond network and water polymerization in membranes of DMPA and DMPC was studied. Principal components analysis of the spectra was used to disclose subtle spectral changes that mirror alterations in the vibrational energy of the water O-H bonds, as a measure of the H-bond network. Increasing temperatures drastically diminished the number of strongly H-bonded water molecules and increase the number of weakly H-bonded waters. This dominating effect of temperature was missing after the subtraction of the pure water spectra from the lipid-containing ones, suggesting the presence of two water populations. An intriguing secondary effect of temperature was also revealed. At the water:lipid interface, phospholipids exhibited an effect qualitatively similar to that of the temperature. DMPA, and particularly DMPC, disrupted the H-bond network in the neighboring water, reducing water polymerization and strengthening the water O-H bonds. The type of the polar head affects the H-bonds more than duplicate the concentration of the lipid from 250 to 500 μM. A relation between head group structure and the effect on the H-bonds network and the existence of two populations of water molecules are discussed. Fil: Wenz, Jorge Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología Buenos Aires Argentina Sociedad Argentina De Investigación Clínica Sociedad Argentina De Investigación Bioquímica Y Biología Molecular Sociedad Argentina De Inmunología Sociedad Argentina De Andrología Sociedad Argentina De Biofísica Sociedad Argentina De Biología Sociedad Argentina De Farmacología Experimental Sociedad Argentina De Fisiología Sociedad Argentina De Hematología Sociedad Argentina De Protozoología |
description |
By exploiting the sensitivity of the NIR spectrum to hydrogen bonds, particularly in the first overtone of water around 1450/55 nm, the influence of temperature and phospholipid head group on the H-bond network and water polymerization in membranes of DMPA and DMPC was studied. Principal components analysis of the spectra was used to disclose subtle spectral changes that mirror alterations in the vibrational energy of the water O-H bonds, as a measure of the H-bond network. Increasing temperatures drastically diminished the number of strongly H-bonded water molecules and increase the number of weakly H-bonded waters. This dominating effect of temperature was missing after the subtraction of the pure water spectra from the lipid-containing ones, suggesting the presence of two water populations. An intriguing secondary effect of temperature was also revealed. At the water:lipid interface, phospholipids exhibited an effect qualitatively similar to that of the temperature. DMPA, and particularly DMPC, disrupted the H-bond network in the neighboring water, reducing water polymerization and strengthening the water O-H bonds. The type of the polar head affects the H-bonds more than duplicate the concentration of the lipid from 250 to 500 μM. A relation between head group structure and the effect on the H-bonds network and the existence of two populations of water molecules are discussed. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/244021 Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 412-412 0076-6046 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/244021 |
identifier_str_mv |
Water and hydrogen bonds in DMPA and DMPC membranes seen from near infrared spectroscopy; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 412-412 0076-6046 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol77-17/Vol.77SuplementoI-2017.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Fundación Revista Medicina |
publisher.none.fl_str_mv |
Fundación Revista Medicina |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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