The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method
- Autores
- Adur, Javier Fernando; D´Souza Li, Lilia; Pedroni, Marcus Vinícius; Steiner, Carlos E.; Pelagati, Vitor B.; de Thomaz, Andre A.; Carvalho, Hernandes F.; Cesar, Carlos L.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The confirmatory diagnosis of Osteogenesis Imperfecta (OI) requires invasive, commonly bone biopsy, time consuming and destructive methods. This paper proposes an alternative method using a combination of two-photon excitation fluorescence (TPEF) and second-harmonic generation (SHG) microscopies from easily obtained human skin biopsies. We show that this method can distinguish subtypes of human OI. Methodology/Principal Findings: Different aspects of collagen microstructure of skin fresh biopsies and standard H&E-stained sections of normal and OI patients (mild and severe forms) were distinguished by TPEF and SHG images. Moreover, important differences between subtypes of OI were identified using different methods of quantification such as collagen density, ratio between collagen and elastic tissue, and gray-level co-occurrence matrix (GLCM) image-pattern analysis. Collagen density was lower in OI dermis, while the SHG/autofluorescence index of the dermis was significantly higher in OI as compared to that of the normal skin. We also showed that the energy value of GLCM texture analysis is useful to discriminate mild from severe OI and from normal skin. Conclusions/Significance: This work demonstrated that nonlinear microscopy techniques in combination with image-analysis approaches represent a powerful tool to investigate the collagen organization in skin dermis in patients with OI and has the potential to distinguish the different types of OI. The procedure outlined in this paper requires a skin biopsy, which is almost painless as compared to the bone biopsy commonly used in conventional methods. The data presented here complement existing clinical diagnostic techniques and can be used as a diagnostic procedure to confirm the disease, evaluate its severity and treatment efficacy.
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Estadual de Campinas; Brasil
Fil: D´Souza Li, Lilia. Universidade Estadual de Campinas; Brasil
Fil: Pedroni, Marcus Vinícius. Universidade Estadual de Campinas; Brasil
Fil: Steiner, Carlos E.. Universidade Estadual de Campinas; Brasil
Fil: Pelagati, Vitor B.. Universidade Estadual de Campinas; Brasil
Fil: de Thomaz, Andre A.. Universidade Estadual de Campinas; Brasil
Fil: Carvalho, Hernandes F.. Universidade Estadual de Campinas; Brasil
Fil: Cesar, Carlos L.. Universidade Estadual de Campinas; Brasil - Materia
-
OSTEOGENESIS IMPERFECTA
NONLINEAR MICROSCOPY
TEXTURE ANALYSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/23785
Ver los metadatos del registro completo
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The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic methodAdur, Javier FernandoD´Souza Li, LiliaPedroni, Marcus ViníciusSteiner, Carlos E.Pelagati, Vitor B.de Thomaz, Andre A.Carvalho, Hernandes F.Cesar, Carlos L.OSTEOGENESIS IMPERFECTANONLINEAR MICROSCOPYTEXTURE ANALYSIShttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Background: The confirmatory diagnosis of Osteogenesis Imperfecta (OI) requires invasive, commonly bone biopsy, time consuming and destructive methods. This paper proposes an alternative method using a combination of two-photon excitation fluorescence (TPEF) and second-harmonic generation (SHG) microscopies from easily obtained human skin biopsies. We show that this method can distinguish subtypes of human OI. Methodology/Principal Findings: Different aspects of collagen microstructure of skin fresh biopsies and standard H&E-stained sections of normal and OI patients (mild and severe forms) were distinguished by TPEF and SHG images. Moreover, important differences between subtypes of OI were identified using different methods of quantification such as collagen density, ratio between collagen and elastic tissue, and gray-level co-occurrence matrix (GLCM) image-pattern analysis. Collagen density was lower in OI dermis, while the SHG/autofluorescence index of the dermis was significantly higher in OI as compared to that of the normal skin. We also showed that the energy value of GLCM texture analysis is useful to discriminate mild from severe OI and from normal skin. Conclusions/Significance: This work demonstrated that nonlinear microscopy techniques in combination with image-analysis approaches represent a powerful tool to investigate the collagen organization in skin dermis in patients with OI and has the potential to distinguish the different types of OI. The procedure outlined in this paper requires a skin biopsy, which is almost painless as compared to the bone biopsy commonly used in conventional methods. The data presented here complement existing clinical diagnostic techniques and can be used as a diagnostic procedure to confirm the disease, evaluate its severity and treatment efficacy.Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Estadual de Campinas; BrasilFil: D´Souza Li, Lilia. Universidade Estadual de Campinas; BrasilFil: Pedroni, Marcus Vinícius. Universidade Estadual de Campinas; BrasilFil: Steiner, Carlos E.. Universidade Estadual de Campinas; BrasilFil: Pelagati, Vitor B.. Universidade Estadual de Campinas; BrasilFil: de Thomaz, Andre A.. Universidade Estadual de Campinas; BrasilFil: Carvalho, Hernandes F.. Universidade Estadual de Campinas; BrasilFil: Cesar, Carlos L.. Universidade Estadual de Campinas; BrasilPublic Library of Science2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23785Adur, Javier Fernando; D´Souza Li, Lilia; Pedroni, Marcus Vinícius; Steiner, Carlos E.; Pelagati, Vitor B.; et al.; The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method; Public Library of Science; Plos One; 8; 7; 7-2013; 1-111932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069186info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0069186info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:37Zoai:ri.conicet.gov.ar:11336/23785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:37.843CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
title |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
spellingShingle |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method Adur, Javier Fernando OSTEOGENESIS IMPERFECTA NONLINEAR MICROSCOPY TEXTURE ANALYSIS |
title_short |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
title_full |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
title_fullStr |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
title_full_unstemmed |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
title_sort |
The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method |
dc.creator.none.fl_str_mv |
Adur, Javier Fernando D´Souza Li, Lilia Pedroni, Marcus Vinícius Steiner, Carlos E. Pelagati, Vitor B. de Thomaz, Andre A. Carvalho, Hernandes F. Cesar, Carlos L. |
author |
Adur, Javier Fernando |
author_facet |
Adur, Javier Fernando D´Souza Li, Lilia Pedroni, Marcus Vinícius Steiner, Carlos E. Pelagati, Vitor B. de Thomaz, Andre A. Carvalho, Hernandes F. Cesar, Carlos L. |
author_role |
author |
author2 |
D´Souza Li, Lilia Pedroni, Marcus Vinícius Steiner, Carlos E. Pelagati, Vitor B. de Thomaz, Andre A. Carvalho, Hernandes F. Cesar, Carlos L. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
OSTEOGENESIS IMPERFECTA NONLINEAR MICROSCOPY TEXTURE ANALYSIS |
topic |
OSTEOGENESIS IMPERFECTA NONLINEAR MICROSCOPY TEXTURE ANALYSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: The confirmatory diagnosis of Osteogenesis Imperfecta (OI) requires invasive, commonly bone biopsy, time consuming and destructive methods. This paper proposes an alternative method using a combination of two-photon excitation fluorescence (TPEF) and second-harmonic generation (SHG) microscopies from easily obtained human skin biopsies. We show that this method can distinguish subtypes of human OI. Methodology/Principal Findings: Different aspects of collagen microstructure of skin fresh biopsies and standard H&E-stained sections of normal and OI patients (mild and severe forms) were distinguished by TPEF and SHG images. Moreover, important differences between subtypes of OI were identified using different methods of quantification such as collagen density, ratio between collagen and elastic tissue, and gray-level co-occurrence matrix (GLCM) image-pattern analysis. Collagen density was lower in OI dermis, while the SHG/autofluorescence index of the dermis was significantly higher in OI as compared to that of the normal skin. We also showed that the energy value of GLCM texture analysis is useful to discriminate mild from severe OI and from normal skin. Conclusions/Significance: This work demonstrated that nonlinear microscopy techniques in combination with image-analysis approaches represent a powerful tool to investigate the collagen organization in skin dermis in patients with OI and has the potential to distinguish the different types of OI. The procedure outlined in this paper requires a skin biopsy, which is almost painless as compared to the bone biopsy commonly used in conventional methods. The data presented here complement existing clinical diagnostic techniques and can be used as a diagnostic procedure to confirm the disease, evaluate its severity and treatment efficacy. Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Estadual de Campinas; Brasil Fil: D´Souza Li, Lilia. Universidade Estadual de Campinas; Brasil Fil: Pedroni, Marcus Vinícius. Universidade Estadual de Campinas; Brasil Fil: Steiner, Carlos E.. Universidade Estadual de Campinas; Brasil Fil: Pelagati, Vitor B.. Universidade Estadual de Campinas; Brasil Fil: de Thomaz, Andre A.. Universidade Estadual de Campinas; Brasil Fil: Carvalho, Hernandes F.. Universidade Estadual de Campinas; Brasil Fil: Cesar, Carlos L.. Universidade Estadual de Campinas; Brasil |
description |
Background: The confirmatory diagnosis of Osteogenesis Imperfecta (OI) requires invasive, commonly bone biopsy, time consuming and destructive methods. This paper proposes an alternative method using a combination of two-photon excitation fluorescence (TPEF) and second-harmonic generation (SHG) microscopies from easily obtained human skin biopsies. We show that this method can distinguish subtypes of human OI. Methodology/Principal Findings: Different aspects of collagen microstructure of skin fresh biopsies and standard H&E-stained sections of normal and OI patients (mild and severe forms) were distinguished by TPEF and SHG images. Moreover, important differences between subtypes of OI were identified using different methods of quantification such as collagen density, ratio between collagen and elastic tissue, and gray-level co-occurrence matrix (GLCM) image-pattern analysis. Collagen density was lower in OI dermis, while the SHG/autofluorescence index of the dermis was significantly higher in OI as compared to that of the normal skin. We also showed that the energy value of GLCM texture analysis is useful to discriminate mild from severe OI and from normal skin. Conclusions/Significance: This work demonstrated that nonlinear microscopy techniques in combination with image-analysis approaches represent a powerful tool to investigate the collagen organization in skin dermis in patients with OI and has the potential to distinguish the different types of OI. The procedure outlined in this paper requires a skin biopsy, which is almost painless as compared to the bone biopsy commonly used in conventional methods. The data presented here complement existing clinical diagnostic techniques and can be used as a diagnostic procedure to confirm the disease, evaluate its severity and treatment efficacy. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/23785 Adur, Javier Fernando; D´Souza Li, Lilia; Pedroni, Marcus Vinícius; Steiner, Carlos E.; Pelagati, Vitor B.; et al.; The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method; Public Library of Science; Plos One; 8; 7; 7-2013; 1-11 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/23785 |
identifier_str_mv |
Adur, Javier Fernando; D´Souza Li, Lilia; Pedroni, Marcus Vinícius; Steiner, Carlos E.; Pelagati, Vitor B.; et al.; The severity of Osteogenesis imperfecta and type I collagen pattern in human skin as determined by nonlinear microscopy: proof of principle of a diagnostic method; Public Library of Science; Plos One; 8; 7; 7-2013; 1-11 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069186 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0069186 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |