Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Autores
Chumbimuni Torres, Karin Y.; Coronado, Ramon E.; Mfuh, Adelphe M.; Castro Guerrero, Carlos; Silva, María Fernanda; Negrete, George R.; Bizios, Rena; Garcia, Carlos D.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.
Fil: Chumbimuni Torres, Karin Y.. The University of Texas at San Antonio; Estados Unidos
Fil: Coronado, Ramon E.. The University of Texas at San Antonio; Estados Unidos
Fil: Mfuh, Adelphe M.. The University of Texas at San Antonio; Estados Unidos
Fil: Castro Guerrero, Carlos. The University of Texas at San Antonio; Estados Unidos
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Negrete, George R.. The University of Texas at San Antonio; Estados Unidos
Fil: Bizios, Rena. The University of Texas at San Antonio; Estados Unidos
Fil: Garcia, Carlos D.. The University of Texas at San Antonio; Estados Unidos
Materia
Proteins
Thin-Films
Pdms
Endothelial Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42439
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42439
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cellsChumbimuni Torres, Karin Y.Coronado, Ramon E.Mfuh, Adelphe M.Castro Guerrero, CarlosSilva, María FernandaNegrete, George R.Bizios, RenaGarcia, Carlos D.ProteinsThin-FilmsPdmsEndothelial Cellshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.Fil: Chumbimuni Torres, Karin Y.. The University of Texas at San Antonio; Estados UnidosFil: Coronado, Ramon E.. The University of Texas at San Antonio; Estados UnidosFil: Mfuh, Adelphe M.. The University of Texas at San Antonio; Estados UnidosFil: Castro Guerrero, Carlos. The University of Texas at San Antonio; Estados UnidosFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Negrete, George R.. The University of Texas at San Antonio; Estados UnidosFil: Bizios, Rena. The University of Texas at San Antonio; Estados UnidosFil: Garcia, Carlos D.. The University of Texas at San Antonio; Estados UnidosRoyal Society of Chemistry2011-08-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42439Chumbimuni Torres, Karin Y.; Coronado, Ramon E.; Mfuh, Adelphe M.; Castro Guerrero, Carlos; Silva, María Fernanda; et al.; Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells; Royal Society of Chemistry; RSC Advances; 1; 4; 31-8-2011; 706-7142046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c1ra00198ainfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2011/RA/c1ra00198ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:57Zoai:ri.conicet.gov.ar:11336/42439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:57.501CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
title Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
spellingShingle Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
Chumbimuni Torres, Karin Y.
Proteins
Thin-Films
Pdms
Endothelial Cells
title_short Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
title_full Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
title_fullStr Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
title_full_unstemmed Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
title_sort Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells
dc.creator.none.fl_str_mv Chumbimuni Torres, Karin Y.
Coronado, Ramon E.
Mfuh, Adelphe M.
Castro Guerrero, Carlos
Silva, María Fernanda
Negrete, George R.
Bizios, Rena
Garcia, Carlos D.
author Chumbimuni Torres, Karin Y.
author_facet Chumbimuni Torres, Karin Y.
Coronado, Ramon E.
Mfuh, Adelphe M.
Castro Guerrero, Carlos
Silva, María Fernanda
Negrete, George R.
Bizios, Rena
Garcia, Carlos D.
author_role author
author2 Coronado, Ramon E.
Mfuh, Adelphe M.
Castro Guerrero, Carlos
Silva, María Fernanda
Negrete, George R.
Bizios, Rena
Garcia, Carlos D.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteins
Thin-Films
Pdms
Endothelial Cells
topic Proteins
Thin-Films
Pdms
Endothelial Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.
Fil: Chumbimuni Torres, Karin Y.. The University of Texas at San Antonio; Estados Unidos
Fil: Coronado, Ramon E.. The University of Texas at San Antonio; Estados Unidos
Fil: Mfuh, Adelphe M.. The University of Texas at San Antonio; Estados Unidos
Fil: Castro Guerrero, Carlos. The University of Texas at San Antonio; Estados Unidos
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Negrete, George R.. The University of Texas at San Antonio; Estados Unidos
Fil: Bizios, Rena. The University of Texas at San Antonio; Estados Unidos
Fil: Garcia, Carlos D.. The University of Texas at San Antonio; Estados Unidos
description This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42439
Chumbimuni Torres, Karin Y.; Coronado, Ramon E.; Mfuh, Adelphe M.; Castro Guerrero, Carlos; Silva, María Fernanda; et al.; Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells; Royal Society of Chemistry; RSC Advances; 1; 4; 31-8-2011; 706-714
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42439
identifier_str_mv Chumbimuni Torres, Karin Y.; Coronado, Ramon E.; Mfuh, Adelphe M.; Castro Guerrero, Carlos; Silva, María Fernanda; et al.; Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells; Royal Society of Chemistry; RSC Advances; 1; 4; 31-8-2011; 706-714
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c1ra00198a
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2011/RA/c1ra00198a
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082855685324800
score 13.22299

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