Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature
- Autores
- Paulucci, Natalia Soledad; Medeot, Daniela Beatriz; Woelke, Mariela Rosana; Dardanelli, Marta Susana; de Lema, M. G.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process. Methods and Results: Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain. Conclusions: In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down-regulated by temperature to maintain membrane fluidity under stressful conditions. Significance and Impact of the Study: This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture.
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medeot, Daniela Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Woelke, Mariela Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Lema, M. G.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina - Materia
-
Aerobic Mechanism
Bradyrhizobium Sp.
Desaturase Gene
Nodulating Peanut
Unsaturated Fatty Acids - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/22120
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/22120 |
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network_name_str |
CONICET Digital (CONICET) |
spelling |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperaturePaulucci, Natalia SoledadMedeot, Daniela BeatrizWoelke, Mariela RosanaDardanelli, Marta Susanade Lema, M. G.Aerobic MechanismBradyrhizobium Sp.Desaturase GeneNodulating PeanutUnsaturated Fatty Acidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process. Methods and Results: Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain. Conclusions: In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down-regulated by temperature to maintain membrane fluidity under stressful conditions. Significance and Impact of the Study: This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture.Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medeot, Daniela Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Woelke, Mariela Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Lema, M. G.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaWiley2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22120Paulucci, Natalia Soledad; Medeot, Daniela Beatriz; Woelke, Mariela Rosana; Dardanelli, Marta Susana; de Lema, M. G.; Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature; Wiley; Journal of Applied Microbiology; 114; 5; 3-2013; 1457-14671364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12155info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12155/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:35Zoai:ri.conicet.gov.ar:11336/22120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:36.028CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
title |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
spellingShingle |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature Paulucci, Natalia Soledad Aerobic Mechanism Bradyrhizobium Sp. Desaturase Gene Nodulating Peanut Unsaturated Fatty Acids |
title_short |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
title_full |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
title_fullStr |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
title_full_unstemmed |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
title_sort |
Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature |
dc.creator.none.fl_str_mv |
Paulucci, Natalia Soledad Medeot, Daniela Beatriz Woelke, Mariela Rosana Dardanelli, Marta Susana de Lema, M. G. |
author |
Paulucci, Natalia Soledad |
author_facet |
Paulucci, Natalia Soledad Medeot, Daniela Beatriz Woelke, Mariela Rosana Dardanelli, Marta Susana de Lema, M. G. |
author_role |
author |
author2 |
Medeot, Daniela Beatriz Woelke, Mariela Rosana Dardanelli, Marta Susana de Lema, M. G. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Aerobic Mechanism Bradyrhizobium Sp. Desaturase Gene Nodulating Peanut Unsaturated Fatty Acids |
topic |
Aerobic Mechanism Bradyrhizobium Sp. Desaturase Gene Nodulating Peanut Unsaturated Fatty Acids |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aims: The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process. Methods and Results: Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain. Conclusions: In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down-regulated by temperature to maintain membrane fluidity under stressful conditions. Significance and Impact of the Study: This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture. Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Medeot, Daniela Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Woelke, Mariela Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: de Lema, M. G.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina |
description |
Aims: The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process. Methods and Results: Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT-PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain. Conclusions: In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down-regulated by temperature to maintain membrane fluidity under stressful conditions. Significance and Impact of the Study: This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/22120 Paulucci, Natalia Soledad; Medeot, Daniela Beatriz; Woelke, Mariela Rosana; Dardanelli, Marta Susana; de Lema, M. G.; Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature; Wiley; Journal of Applied Microbiology; 114; 5; 3-2013; 1457-1467 1364-5072 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/22120 |
identifier_str_mv |
Paulucci, Natalia Soledad; Medeot, Daniela Beatriz; Woelke, Mariela Rosana; Dardanelli, Marta Susana; de Lema, M. G.; Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature; Wiley; Journal of Applied Microbiology; 114; 5; 3-2013; 1457-1467 1364-5072 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12155 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12155/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.069144 |