Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella

Autores
Ibáñez, María Marta; Cerminati, Sebastián; Checa, Susana Karina; Soncini, Fernando Carlos
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two homologous transcription factors, CueR and GolS, that belong to the MerR metalloregulatory family are responsible for Salmonella Cu and Au sensing and resistance, respectively. They share similarities not only in their sequences, but also in their target transcription binding sites. While CueR responds similarly to Au, Ag, or Cu to induce the expression of its target genes, GolS shows higher activation by Au than by Ag or Cu. We showed that the ability of GolS to distinguish Au from Cu resides in the metal-binding loop motif. Here, we identify the amino acids within the motif that determine in vivo metal selectivity. We show that residues at positions 113 and 118 within the metal-binding loop are the main contributors to metal selectivity. The presence of a Pro residue at position 113 favors the detection of Cu, while the presence of Pro at position 118 disfavors it. Our results highlight the molecular bases that allow these regulators to coordinate the correct metal ion directing the response to a particular metal injury.
Fil: Ibáñez, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cerminati, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Checa, Susana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Selectividad Metalica
Reguladores Merr
Regulacion Transcripcional
Iones Metalicos Monovalentes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7606
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in SalmonellaIbáñez, María MartaCerminati, SebastiánCheca, Susana KarinaSoncini, Fernando CarlosSelectividad MetalicaReguladores MerrRegulacion TranscripcionalIones Metalicos Monovalenteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two homologous transcription factors, CueR and GolS, that belong to the MerR metalloregulatory family are responsible for Salmonella Cu and Au sensing and resistance, respectively. They share similarities not only in their sequences, but also in their target transcription binding sites. While CueR responds similarly to Au, Ag, or Cu to induce the expression of its target genes, GolS shows higher activation by Au than by Ag or Cu. We showed that the ability of GolS to distinguish Au from Cu resides in the metal-binding loop motif. Here, we identify the amino acids within the motif that determine in vivo metal selectivity. We show that residues at positions 113 and 118 within the metal-binding loop are the main contributors to metal selectivity. The presence of a Pro residue at position 113 favors the detection of Cu, while the presence of Pro at position 118 disfavors it. Our results highlight the molecular bases that allow these regulators to coordinate the correct metal ion directing the response to a particular metal injury.Fil: Ibáñez, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Cerminati, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Checa, Susana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaAmerican Society For Microbiology2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7606Ibáñez, María Marta; Cerminati, Sebastián; Checa, Susana Karina; Soncini, Fernando Carlos; Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella; American Society For Microbiology; Journal Of Bacteriology; 195; 13; 7-2013; 3084-30920021-9193enginfo:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/195/13/3084.longinfo:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00153-13info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697532/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:58Zoai:ri.conicet.gov.ar:11336/7606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:58.814CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
title Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
spellingShingle Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
Ibáñez, María Marta
Selectividad Metalica
Reguladores Merr
Regulacion Transcripcional
Iones Metalicos Monovalentes
title_short Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
title_full Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
title_fullStr Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
title_full_unstemmed Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
title_sort Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella
dc.creator.none.fl_str_mv Ibáñez, María Marta
Cerminati, Sebastián
Checa, Susana Karina
Soncini, Fernando Carlos
author Ibáñez, María Marta
author_facet Ibáñez, María Marta
Cerminati, Sebastián
Checa, Susana Karina
Soncini, Fernando Carlos
author_role author
author2 Cerminati, Sebastián
Checa, Susana Karina
Soncini, Fernando Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv Selectividad Metalica
Reguladores Merr
Regulacion Transcripcional
Iones Metalicos Monovalentes
topic Selectividad Metalica
Reguladores Merr
Regulacion Transcripcional
Iones Metalicos Monovalentes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two homologous transcription factors, CueR and GolS, that belong to the MerR metalloregulatory family are responsible for Salmonella Cu and Au sensing and resistance, respectively. They share similarities not only in their sequences, but also in their target transcription binding sites. While CueR responds similarly to Au, Ag, or Cu to induce the expression of its target genes, GolS shows higher activation by Au than by Ag or Cu. We showed that the ability of GolS to distinguish Au from Cu resides in the metal-binding loop motif. Here, we identify the amino acids within the motif that determine in vivo metal selectivity. We show that residues at positions 113 and 118 within the metal-binding loop are the main contributors to metal selectivity. The presence of a Pro residue at position 113 favors the detection of Cu, while the presence of Pro at position 118 disfavors it. Our results highlight the molecular bases that allow these regulators to coordinate the correct metal ion directing the response to a particular metal injury.
Fil: Ibáñez, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Cerminati, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Checa, Susana Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Two homologous transcription factors, CueR and GolS, that belong to the MerR metalloregulatory family are responsible for Salmonella Cu and Au sensing and resistance, respectively. They share similarities not only in their sequences, but also in their target transcription binding sites. While CueR responds similarly to Au, Ag, or Cu to induce the expression of its target genes, GolS shows higher activation by Au than by Ag or Cu. We showed that the ability of GolS to distinguish Au from Cu resides in the metal-binding loop motif. Here, we identify the amino acids within the motif that determine in vivo metal selectivity. We show that residues at positions 113 and 118 within the metal-binding loop are the main contributors to metal selectivity. The presence of a Pro residue at position 113 favors the detection of Cu, while the presence of Pro at position 118 disfavors it. Our results highlight the molecular bases that allow these regulators to coordinate the correct metal ion directing the response to a particular metal injury.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7606
Ibáñez, María Marta; Cerminati, Sebastián; Checa, Susana Karina; Soncini, Fernando Carlos; Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella; American Society For Microbiology; Journal Of Bacteriology; 195; 13; 7-2013; 3084-3092
0021-9193
url http://hdl.handle.net/11336/7606
identifier_str_mv Ibáñez, María Marta; Cerminati, Sebastián; Checa, Susana Karina; Soncini, Fernando Carlos; Dissecting the Metal Selectivity of MerR Monovalent Metal Ion Sensors in Salmonella; American Society For Microbiology; Journal Of Bacteriology; 195; 13; 7-2013; 3084-3092
0021-9193
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/195/13/3084.long
info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.00153-13
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697532/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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