MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

• Autores: Dueholm, Morten Kam Dahl; Nierychlo, Marta; Andersen, Kasper Skytte; Rudkjøbing, Vibeke; Knutsson, Simon; Arriaga, Sonia; Bakke, Rune; Boon, Nico; Bux, Faizal; Christensson, Magnus; Chua, Adeline Seak May; Curtis, Thomas P.; Cytryn, Eddie; Erijman, Leonardo; Etchebehere, Claudia; Fatta Kassinos, Despo; Frigon, Dominic; Garcia Chaves, Maria Carolina; Gu, April Z.; Horn, Harald; Jenkins, David; Kreuzinger, Norbert; Kumari, Sheena; Lanham, Ana; Law, Yingyu; Leiknes, TorOve; Morgenroth, Eberhard; Muszyński, Adam; Petrovski, Steve; Pijuan, Maite; Pillai, Suraj Babu; Reis, Maria A. M.; Rong, Qi; Rossetti, Simona; Seviour, Robert; Tooker, Nick; Vainio, Pirjo; van Loosdrecht, Mark; Vikraman, R.; Wanner, Jiří; Weissbrodt, David; Wen, Xianghua; Zhang, Tong; Nielsen, Per H.; Albertsen, Mads; Nielsen, Per Halkjær
• Año de publicación: 2022
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Activated sludge; 16S rRNA gene database
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’ database. MiDAS 4 is an amplicon sequence variant resolved, full-length 16S rRNA gene reference database with a comprehensive taxonomy from domain to species level for all sequences. We use an independent dataset (269 WWTPs) to show that MiDAS 4, compared to commonly used universal reference databases, provides a better coverage for WWTP bacteria and an improved rate of genus and species level classification. Taking advantage of MiDAS 4, we carry out an amplicon-based, global-scale microbial community profiling of activated sludge plants using two common sets of primers targeting regions of the 16S rRNA gene, revealing how environmental conditions and biogeography shape the activated sludge microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 966 genera and 1530 species that represent approximately 80% and 50% of the accumulated read abundance, respectively. Finally, we show that for well-studied functional guilds, such as nitrifiers or polyphosphate-accumulating organisms, the same genera are prevalent worldwide, with only a few abundant species in each genus.
Fil: Dueholm, Morten Kam Dahl. Aalborg University; Dinamarca
Fil: Nierychlo, Marta. Aalborg University; Dinamarca
Fil: Andersen, Kasper Skytte. Aalborg University; Dinamarca
Fil: Rudkjøbing, Vibeke. Aalborg University; Dinamarca
Fil: Knutsson, Simon. Aalborg University; Dinamarca
Fil: Arriaga, Sonia. Instituto Potosino de Investigación Científica y Tecnológica; México
Fil: Bakke, Rune. University College of Southeast Norway; Noruega
Fil: Boon, Nico. University of Ghent; Bélgica
Fil: Bux, Faizal. Durban University of Technology; Sudáfrica
Fil: Christensson, Magnus. Veolia Water Technologies Ab; Suecia
Fil: Chua, Adeline Seak May. University Malaya; Malasia
Fil: Curtis, Thomas P.. University of Newcastle; Reino Unido
Fil: Cytryn, Eddie. Agricultural Research Organization Of Israel; Israel
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
Fil: Etchebehere, Claudia. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Fatta Kassinos, Despo. University of Cyprus; Chipre
Fil: Frigon, Dominic. McGill University; Canadá
Fil: Garcia Chaves, Maria Carolina. Universidad de Antioquia; Colombia
Fil: Gu, April Z.. Cornell University; Estados Unidos
Fil: Horn, Harald. Karlsruher Institut Für Technologie; Alemania
Fil: Jenkins, David. David Jenkins & Associates Inc; Estados Unidos
Fil: Kreuzinger, Norbert. Tu Wien; Austria
Fil: Kumari, Sheena. Durban University of Technology; Sudáfrica
Fil: Lanham, Ana. University of Bath; Reino Unido
Fil: Law, Yingyu. Singapore Centre For Environmental Life Sciences Engineering; Singapur
Fil: Leiknes, TorOve. King Abdullah University of Science and Technology; Arabia Saudita
Fil: Morgenroth, Eberhard. Eth Zürich; Suiza
Fil: Muszyński, Adam. Politechnika Warszawska; Polonia
Fil: Petrovski, Steve. La Trobe University; Australia
Fil: Pijuan, Maite. Catalan Institute For Water Research; España
Fil: Pillai, Suraj Babu. Va Tech Wabag Ltd; India
Fil: Reis, Maria A. M.. Universidade Nova de Lisboa; Portugal
Fil: Rong, Qi. Chinese Academy of Sciences; China
Fil: Rossetti, Simona. Istituto Di Ricerca Sulle Acque (irsa) ; Consiglio Nazionale Delle Ricerche;
Fil: Seviour, Robert. La Trobe University; Australia
Fil: Tooker, Nick. University of Massachussets; Estados Unidos
Fil: Vainio, Pirjo. Espoo R&D Center; Finlandia
Fil: van Loosdrecht, Mark. Delft University of Technology; Países Bajos
Fil: Vikraman, R.. VA Tech Wabag, Philippines Inc; Filipinas
Fil: Wanner, Jiří. University of Chemistry And Technology; República Checa
Fil: Weissbrodt, David. Delft University of Technology; Países Bajos
Fil: Wen, Xianghua. Tsinghua University; China
Fil: Zhang, Tong. The University of Hong Kong; Hong Kong
Fil: Nielsen, Per H.. Aalborg University; Dinamarca
Fil: Albertsen, Mads. Aalborg University; Dinamarca
Fil: Nielsen, Per Halkjær. Aalborg University; Dinamarca