The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics
- Autores
- Schulze, Stefan; Adams, Zachary; Cerletti, Micaela; de Castro, Rosana Esther; Ferreira Cerca, Sébastien; Fufezan, Christian; Gimenez, Maria Ines; Hippler, Michael; Jevtic, Zivojin; Knüppel, Robert; Legerme, Georgio; Lenz, Christof; Marchfelder, Anita; Maupin Furlow, Julie; Paggi, Roberto Alejandro; Pfeiffer, Friedhelm; Poetsch, Ansgar; Urlaub, Henning; Pohlschroder, Mechthild
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying > 72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics.
Fil: Schulze, Stefan. University of Pennsylvania; Estados Unidos
Fil: Adams, Zachary. University of Florida; Estados Unidos
Fil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Ferreira Cerca, Sébastien. Universitat Regensburg; Alemania
Fil: Fufezan, Christian. Heidelberg University; Alemania
Fil: Gimenez, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hippler, Michael. University Of Münster; Alemania
Fil: Jevtic, Zivojin. Max Planck Institute For Biophysical Chemistry; Alemania
Fil: Knüppel, Robert. Universitat Regensburg; Alemania
Fil: Legerme, Georgio. University of Pennsylvania; Estados Unidos
Fil: Lenz, Christof. Max Planck Institute For Biophysical Chemistry; Alemania
Fil: Marchfelder, Anita. Universitat Ulm; Alemania
Fil: Maupin Furlow, Julie. University of Florida; Estados Unidos
Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pfeiffer, Friedhelm. Max Planck Institute Of Biochemistry.; Alemania
Fil: Poetsch, Ansgar. Ruhr University Bochum; Alemania
Fil: Urlaub, Henning. Max Planck Institute For Biophysical Chemistry; Alemania
Fil: Pohlschroder, Mechthild. University of Pennsylvania; Estados Unidos - Materia
-
ARCHAEA
PROTEOME PROJECT
ARCHAEAL BIOLOGY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140819
Ver los metadatos del registro completo
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The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomicsSchulze, StefanAdams, ZacharyCerletti, Micaelade Castro, Rosana EstherFerreira Cerca, SébastienFufezan, ChristianGimenez, Maria InesHippler, MichaelJevtic, ZivojinKnüppel, RobertLegerme, GeorgioLenz, ChristofMarchfelder, AnitaMaupin Furlow, JuliePaggi, Roberto AlejandroPfeiffer, FriedhelmPoetsch, AnsgarUrlaub, HenningPohlschroder, MechthildARCHAEAPROTEOME PROJECTARCHAEAL BIOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying > 72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics.Fil: Schulze, Stefan. University of Pennsylvania; Estados UnidosFil: Adams, Zachary. University of Florida; Estados UnidosFil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Ferreira Cerca, Sébastien. Universitat Regensburg; AlemaniaFil: Fufezan, Christian. Heidelberg University; AlemaniaFil: Gimenez, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hippler, Michael. University Of Münster; AlemaniaFil: Jevtic, Zivojin. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Knüppel, Robert. Universitat Regensburg; AlemaniaFil: Legerme, Georgio. University of Pennsylvania; Estados UnidosFil: Lenz, Christof. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Marchfelder, Anita. Universitat Ulm; AlemaniaFil: Maupin Furlow, Julie. University of Florida; Estados UnidosFil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pfeiffer, Friedhelm. Max Planck Institute Of Biochemistry.; AlemaniaFil: Poetsch, Ansgar. Ruhr University Bochum; AlemaniaFil: Urlaub, Henning. Max Planck Institute For Biophysical Chemistry; AlemaniaFil: Pohlschroder, Mechthild. University of Pennsylvania; Estados UnidosNature Publishing Group2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140819Schulze, Stefan; Adams, Zachary; Cerletti, Micaela; de Castro, Rosana Esther; Ferreira Cerca, Sébastien; et al.; The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics; Nature Publishing Group; Nature Communications; 11; 1; 6-2020; 1-142041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41467-020-16784-7info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-020-16784-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:50Zoai:ri.conicet.gov.ar:11336/140819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:50.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| title |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| spellingShingle |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics Schulze, Stefan ARCHAEA PROTEOME PROJECT ARCHAEAL BIOLOGY |
| title_short |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| title_full |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| title_fullStr |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| title_full_unstemmed |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| title_sort |
The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
| dc.creator.none.fl_str_mv |
Schulze, Stefan Adams, Zachary Cerletti, Micaela de Castro, Rosana Esther Ferreira Cerca, Sébastien Fufezan, Christian Gimenez, Maria Ines Hippler, Michael Jevtic, Zivojin Knüppel, Robert Legerme, Georgio Lenz, Christof Marchfelder, Anita Maupin Furlow, Julie Paggi, Roberto Alejandro Pfeiffer, Friedhelm Poetsch, Ansgar Urlaub, Henning Pohlschroder, Mechthild |
| author |
Schulze, Stefan |
| author_facet |
Schulze, Stefan Adams, Zachary Cerletti, Micaela de Castro, Rosana Esther Ferreira Cerca, Sébastien Fufezan, Christian Gimenez, Maria Ines Hippler, Michael Jevtic, Zivojin Knüppel, Robert Legerme, Georgio Lenz, Christof Marchfelder, Anita Maupin Furlow, Julie Paggi, Roberto Alejandro Pfeiffer, Friedhelm Poetsch, Ansgar Urlaub, Henning Pohlschroder, Mechthild |
| author_role |
author |
| author2 |
Adams, Zachary Cerletti, Micaela de Castro, Rosana Esther Ferreira Cerca, Sébastien Fufezan, Christian Gimenez, Maria Ines Hippler, Michael Jevtic, Zivojin Knüppel, Robert Legerme, Georgio Lenz, Christof Marchfelder, Anita Maupin Furlow, Julie Paggi, Roberto Alejandro Pfeiffer, Friedhelm Poetsch, Ansgar Urlaub, Henning Pohlschroder, Mechthild |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ARCHAEA PROTEOME PROJECT ARCHAEAL BIOLOGY |
| topic |
ARCHAEA PROTEOME PROJECT ARCHAEAL BIOLOGY |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying > 72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics. Fil: Schulze, Stefan. University of Pennsylvania; Estados Unidos Fil: Adams, Zachary. University of Florida; Estados Unidos Fil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Ferreira Cerca, Sébastien. Universitat Regensburg; Alemania Fil: Fufezan, Christian. Heidelberg University; Alemania Fil: Gimenez, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Hippler, Michael. University Of Münster; Alemania Fil: Jevtic, Zivojin. Max Planck Institute For Biophysical Chemistry; Alemania Fil: Knüppel, Robert. Universitat Regensburg; Alemania Fil: Legerme, Georgio. University of Pennsylvania; Estados Unidos Fil: Lenz, Christof. Max Planck Institute For Biophysical Chemistry; Alemania Fil: Marchfelder, Anita. Universitat Ulm; Alemania Fil: Maupin Furlow, Julie. University of Florida; Estados Unidos Fil: Paggi, Roberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Pfeiffer, Friedhelm. Max Planck Institute Of Biochemistry.; Alemania Fil: Poetsch, Ansgar. Ruhr University Bochum; Alemania Fil: Urlaub, Henning. Max Planck Institute For Biophysical Chemistry; Alemania Fil: Pohlschroder, Mechthild. University of Pennsylvania; Estados Unidos |
| description |
While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying > 72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/140819 Schulze, Stefan; Adams, Zachary; Cerletti, Micaela; de Castro, Rosana Esther; Ferreira Cerca, Sébastien; et al.; The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics; Nature Publishing Group; Nature Communications; 11; 1; 6-2020; 1-14 2041-1723 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/140819 |
| identifier_str_mv |
Schulze, Stefan; Adams, Zachary; Cerletti, Micaela; de Castro, Rosana Esther; Ferreira Cerca, Sébastien; et al.; The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics; Nature Publishing Group; Nature Communications; 11; 1; 6-2020; 1-14 2041-1723 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41467-020-16784-7 info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-020-16784-7 |
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Nature Publishing Group |
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Nature Publishing Group |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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