Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
- Autores
- Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; Dolghih, Elena; Turjanski, Adrian; Roitberg, Adrián; Priestap, Horacio; Perez, Mario; Mackenzie, Lucy; Li, Yiping; Noriega, Fernando G.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.
Fil: Mayoral, Jaime G.. Florida International University; Estados Unidos
Fil: Nouzova, Marcela. Florida International University; Estados Unidos
Fil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; Japón
Fil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; Japón
Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; México
Fil: Dolghih, Elena. University of Florida; Estados Unidos
Fil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roitberg, Adrián. University of Florida; Estados Unidos
Fil: Priestap, Horacio. Florida International University; Estados Unidos
Fil: Perez, Mario. Florida International University; Estados Unidos
Fil: Mackenzie, Lucy. Florida International University; Estados Unidos
Fil: Li, Yiping. Florida International University; Estados Unidos
Fil: Noriega, Fernando G.. Florida International University; Estados Unidos - Materia
-
Aedes Aegypti
Farnesoic Acid
Juvenile Hormone
Methyltransferase
Mosquito - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/72309
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/72309 |
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CONICETDig |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoesMayoral, Jaime G.Nouzova, MarcelaYoshiyama, MichiyoShinoda, TetsuroHernandez Martinez, SalvadorDolghih, ElenaTurjanski, AdrianRoitberg, AdriánPriestap, HoracioPerez, MarioMackenzie, LucyLi, YipingNoriega, Fernando G.Aedes AegyptiFarnesoic AcidJuvenile HormoneMethyltransferaseMosquitohttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.Fil: Mayoral, Jaime G.. Florida International University; Estados UnidosFil: Nouzova, Marcela. Florida International University; Estados UnidosFil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; JapónFil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; JapónFil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; MéxicoFil: Dolghih, Elena. University of Florida; Estados UnidosFil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Roitberg, Adrián. University of Florida; Estados UnidosFil: Priestap, Horacio. Florida International University; Estados UnidosFil: Perez, Mario. Florida International University; Estados UnidosFil: Mackenzie, Lucy. Florida International University; Estados UnidosFil: Li, Yiping. Florida International University; Estados UnidosFil: Noriega, Fernando G.. Florida International University; Estados UnidosPergamon-Elsevier Science Ltd2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72309Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-370965-1748CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2008.09.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174808001719info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727726/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:30Zoai:ri.conicet.gov.ar:11336/72309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:30.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
title |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
spellingShingle |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes Mayoral, Jaime G. Aedes Aegypti Farnesoic Acid Juvenile Hormone Methyltransferase Mosquito |
title_short |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
title_full |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
title_fullStr |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
title_full_unstemmed |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
title_sort |
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes |
dc.creator.none.fl_str_mv |
Mayoral, Jaime G. Nouzova, Marcela Yoshiyama, Michiyo Shinoda, Tetsuro Hernandez Martinez, Salvador Dolghih, Elena Turjanski, Adrian Roitberg, Adrián Priestap, Horacio Perez, Mario Mackenzie, Lucy Li, Yiping Noriega, Fernando G. |
author |
Mayoral, Jaime G. |
author_facet |
Mayoral, Jaime G. Nouzova, Marcela Yoshiyama, Michiyo Shinoda, Tetsuro Hernandez Martinez, Salvador Dolghih, Elena Turjanski, Adrian Roitberg, Adrián Priestap, Horacio Perez, Mario Mackenzie, Lucy Li, Yiping Noriega, Fernando G. |
author_role |
author |
author2 |
Nouzova, Marcela Yoshiyama, Michiyo Shinoda, Tetsuro Hernandez Martinez, Salvador Dolghih, Elena Turjanski, Adrian Roitberg, Adrián Priestap, Horacio Perez, Mario Mackenzie, Lucy Li, Yiping Noriega, Fernando G. |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Aedes Aegypti Farnesoic Acid Juvenile Hormone Methyltransferase Mosquito |
topic |
Aedes Aegypti Farnesoic Acid Juvenile Hormone Methyltransferase Mosquito |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway. Fil: Mayoral, Jaime G.. Florida International University; Estados Unidos Fil: Nouzova, Marcela. Florida International University; Estados Unidos Fil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; Japón Fil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; Japón Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; México Fil: Dolghih, Elena. University of Florida; Estados Unidos Fil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Roitberg, Adrián. University of Florida; Estados Unidos Fil: Priestap, Horacio. Florida International University; Estados Unidos Fil: Perez, Mario. Florida International University; Estados Unidos Fil: Mackenzie, Lucy. Florida International University; Estados Unidos Fil: Li, Yiping. Florida International University; Estados Unidos Fil: Noriega, Fernando G.. Florida International University; Estados Unidos |
description |
A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/72309 Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-37 0965-1748 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/72309 |
identifier_str_mv |
Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-37 0965-1748 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2008.09.010 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174808001719 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727726/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613451986501632 |
score |
13.070432 |