Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes

Autores
Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; Dolghih, Elena; Turjanski, Adrian; Roitberg, Adrián; Priestap, Horacio; Perez, Mario; Mackenzie, Lucy; Li, Yiping; Noriega, Fernando G.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.
Fil: Mayoral, Jaime G.. Florida International University; Estados Unidos
Fil: Nouzova, Marcela. Florida International University; Estados Unidos
Fil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; Japón
Fil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; Japón
Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; México
Fil: Dolghih, Elena. University of Florida; Estados Unidos
Fil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roitberg, Adrián. University of Florida; Estados Unidos
Fil: Priestap, Horacio. Florida International University; Estados Unidos
Fil: Perez, Mario. Florida International University; Estados Unidos
Fil: Mackenzie, Lucy. Florida International University; Estados Unidos
Fil: Li, Yiping. Florida International University; Estados Unidos
Fil: Noriega, Fernando G.. Florida International University; Estados Unidos
Materia
Aedes Aegypti
Farnesoic Acid
Juvenile Hormone
Methyltransferase
Mosquito
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72309
Acceder
id CONICETDig_45dc9742d3a8a459a5f389bf31ea5695
oai_identifier_str oai:ri.conicet.gov.ar:11336/72309
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoesMayoral, Jaime G.Nouzova, MarcelaYoshiyama, MichiyoShinoda, TetsuroHernandez Martinez, SalvadorDolghih, ElenaTurjanski, AdrianRoitberg, AdriánPriestap, HoracioPerez, MarioMackenzie, LucyLi, YipingNoriega, Fernando G.Aedes AegyptiFarnesoic AcidJuvenile HormoneMethyltransferaseMosquitohttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.Fil: Mayoral, Jaime G.. Florida International University; Estados UnidosFil: Nouzova, Marcela. Florida International University; Estados UnidosFil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; JapónFil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; JapónFil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; MéxicoFil: Dolghih, Elena. University of Florida; Estados UnidosFil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Roitberg, Adrián. University of Florida; Estados UnidosFil: Priestap, Horacio. Florida International University; Estados UnidosFil: Perez, Mario. Florida International University; Estados UnidosFil: Mackenzie, Lucy. Florida International University; Estados UnidosFil: Li, Yiping. Florida International University; Estados UnidosFil: Noriega, Fernando G.. Florida International University; Estados UnidosPergamon-Elsevier Science Ltd2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72309Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-370965-1748CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2008.09.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174808001719info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727726/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:19Zoai:ri.conicet.gov.ar:11336/72309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:20.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
title Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
spellingShingle Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
Mayoral, Jaime G.
Aedes Aegypti
Farnesoic Acid
Juvenile Hormone
Methyltransferase
Mosquito
title_short Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
title_full Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
title_fullStr Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
title_full_unstemmed Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
title_sort Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes
dc.creator.none.fl_str_mv Mayoral, Jaime G.
Nouzova, Marcela
Yoshiyama, Michiyo
Shinoda, Tetsuro
Hernandez Martinez, Salvador
Dolghih, Elena
Turjanski, Adrian
Roitberg, Adrián
Priestap, Horacio
Perez, Mario
Mackenzie, Lucy
Li, Yiping
Noriega, Fernando G.
author Mayoral, Jaime G.
author_facet Mayoral, Jaime G.
Nouzova, Marcela
Yoshiyama, Michiyo
Shinoda, Tetsuro
Hernandez Martinez, Salvador
Dolghih, Elena
Turjanski, Adrian
Roitberg, Adrián
Priestap, Horacio
Perez, Mario
Mackenzie, Lucy
Li, Yiping
Noriega, Fernando G.
author_role author
author2 Nouzova, Marcela
Yoshiyama, Michiyo
Shinoda, Tetsuro
Hernandez Martinez, Salvador
Dolghih, Elena
Turjanski, Adrian
Roitberg, Adrián
Priestap, Horacio
Perez, Mario
Mackenzie, Lucy
Li, Yiping
Noriega, Fernando G.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aedes Aegypti
Farnesoic Acid
Juvenile Hormone
Methyltransferase
Mosquito
topic Aedes Aegypti
Farnesoic Acid
Juvenile Hormone
Methyltransferase
Mosquito
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.
Fil: Mayoral, Jaime G.. Florida International University; Estados Unidos
Fil: Nouzova, Marcela. Florida International University; Estados Unidos
Fil: Yoshiyama, Michiyo. National Institute of Agrobiological Sciences; Japón
Fil: Shinoda, Tetsuro. National Institute of Agrobiological Sciences; Japón
Fil: Hernandez Martinez, Salvador. Instituto Nacional de Salud Publica; México
Fil: Dolghih, Elena. University of Florida; Estados Unidos
Fil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roitberg, Adrián. University of Florida; Estados Unidos
Fil: Priestap, Horacio. Florida International University; Estados Unidos
Fil: Perez, Mario. Florida International University; Estados Unidos
Fil: Mackenzie, Lucy. Florida International University; Estados Unidos
Fil: Li, Yiping. Florida International University; Estados Unidos
Fil: Noriega, Fernando G.. Florida International University; Estados Unidos
description A juvenile hormone acid methyltransferase (JHAMT) was isolated as an abundant EST in a library of the corpora allata of the adult female mosquito Aedes aegypti. Its full length cDNA encodes a 278-aa protein that has 43% amino acid identity with BmJHAMT, a juvenile hormone acid methyltransferase previously cloned from Bombyx mori. Heterologous expression produced a recombinant protein that metabolizes farnesoic acid (FA) into methyl farnesoate, as well as juvenile hormone acid into juvenile hormone III (JH III) with exquisite stereo specificity. Real time PCR experiments showed that JHAMT mRNA levels are not an unequivocal indicator of JH III synthesis rates; the A. aegypti JHAMT gene, silent in female pupae, was transcriptionally activated just 4-6 h before adult eclosion. Radiochemical methyltransferase assays using active and inactive corpora allata glands (CA) dissected from sugar and blood-fed females respectively, clearly indicated that significant levels of JHAMT enzymatic activity are present when the CA shows very low spontaneous rates of JH III synthesis. Having the last enzymes of the JH synthetic pathway readily available all the time might be critical for the adult female mosquito to sustain rapid dynamic changes in JH III synthesis in response to nutritional changes or peripheral influences, such as mating or feeding. These results suggest that this gene has different roles in the regulation of JH synthesis in pupal and adult female mosquitoes, and support the hypothesis that the rate-limiting steps in JH III synthesis in adult female mosquitoes are located before entrance of FA into the synthetic pathway.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72309
Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-37
0965-1748
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72309
identifier_str_mv Mayoral, Jaime G.; Nouzova, Marcela; Yoshiyama, Michiyo; Shinoda, Tetsuro; Hernandez Martinez, Salvador; et al.; Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes; Pergamon-Elsevier Science Ltd; Insect Biochem. Molec. Biol.; 39; 1; 1-2009; 31-37
0965-1748
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibmb.2008.09.010
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0965174808001719
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727726/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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