Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations

Autores
Martínez Rincón, Raúl O.; Rivera Pérez, Crisalejandra; Diambra, Luis Anibal; Noriega, Fernando Gabriel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Juvenile hormone (JH) regulates development and reproductive maturation in insects. Thecorpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, whichhave been linked to the ovarian development and are influenced by nutritional signals. Therate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, whichis the outcome of a complex interplay of changes in precursor pools and enzyme levels. Acomprehensive study of the changes in enzymatic activities and precursor pool sizes havebeen previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In thepresent studies, we used two different quantitative approaches to describe and predict howchanges in the individual metabolic reactions in the pathway affect JH synthesis. First, weconstructed generalized additive models (GAMs) that described the association betweenchanges in specific metabolite concentrations with changes in enzymatic activities and sub-strate concentrations. Changes in substrate concentrations explained 50% or more of themodel deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzy-matic activities almost always improved the fitness of GAMs built solely based on substrateconcentrations. GAMs were validated using experimental data that were not included whenthe model was built. In addition, a system of ordinary differential equations (ODE) wasdeveloped to describe the instantaneous changes in metabolites as a function of the levelsof enzymatic catalytic activities. The results demonstrated the ability of the models to predictchanges in the flux of metabolites in the JH pathway, and can be used in the future to designand validate experimental manipulations of JH synthesis.
Fil: Martínez Rincón, Raúl O.. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
Fil: Rivera Pérez, Crisalejandra. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
Fil: Diambra, Luis Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Noriega, Fernando Gabriel. Florida International University; Estados Unidos
Materia
JUVENILE HORMONE SYNTHESIS
MODELING BIOSYNTHESIS
AEDES AEGYPTI
MEVALONIC ACID PATHWAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78966
Acceder
id CONICETDig_19c4f147ac4b6e94b751961ea71f3bee
oai_identifier_str oai:ri.conicet.gov.ar:11336/78966
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equationsMartínez Rincón, Raúl O.Rivera Pérez, CrisalejandraDiambra, Luis AnibalNoriega, Fernando GabrielJUVENILE HORMONE SYNTHESISMODELING BIOSYNTHESISAEDES AEGYPTIMEVALONIC ACID PATHWAYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Juvenile hormone (JH) regulates development and reproductive maturation in insects. Thecorpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, whichhave been linked to the ovarian development and are influenced by nutritional signals. Therate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, whichis the outcome of a complex interplay of changes in precursor pools and enzyme levels. Acomprehensive study of the changes in enzymatic activities and precursor pool sizes havebeen previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In thepresent studies, we used two different quantitative approaches to describe and predict howchanges in the individual metabolic reactions in the pathway affect JH synthesis. First, weconstructed generalized additive models (GAMs) that described the association betweenchanges in specific metabolite concentrations with changes in enzymatic activities and sub-strate concentrations. Changes in substrate concentrations explained 50% or more of themodel deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzy-matic activities almost always improved the fitness of GAMs built solely based on substrateconcentrations. GAMs were validated using experimental data that were not included whenthe model was built. In addition, a system of ordinary differential equations (ODE) wasdeveloped to describe the instantaneous changes in metabolites as a function of the levelsof enzymatic catalytic activities. The results demonstrated the ability of the models to predictchanges in the flux of metabolites in the JH pathway, and can be used in the future to designand validate experimental manipulations of JH synthesis.Fil: Martínez Rincón, Raúl O.. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; MéxicoFil: Rivera Pérez, Crisalejandra. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; MéxicoFil: Diambra, Luis Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Noriega, Fernando Gabriel. Florida International University; Estados UnidosPublic Library of Science2017-02-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78966Martínez Rincón, Raúl O.; Rivera Pérez, Crisalejandra; Diambra, Luis Anibal; Noriega, Fernando Gabriel; Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations; Public Library of Science; Plos One; 12; 2; 3-2-2017; 1-211932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1371/journal.pone.0171516info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171516info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:12Zoai:ri.conicet.gov.ar:11336/78966instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:12.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
title Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
spellingShingle Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
Martínez Rincón, Raúl O.
JUVENILE HORMONE SYNTHESIS
MODELING BIOSYNTHESIS
AEDES AEGYPTI
MEVALONIC ACID PATHWAY
title_short Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
title_full Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
title_fullStr Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
title_full_unstemmed Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
title_sort Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations
dc.creator.none.fl_str_mv Martínez Rincón, Raúl O.
Rivera Pérez, Crisalejandra
Diambra, Luis Anibal
Noriega, Fernando Gabriel
author Martínez Rincón, Raúl O.
author_facet Martínez Rincón, Raúl O.
Rivera Pérez, Crisalejandra
Diambra, Luis Anibal
Noriega, Fernando Gabriel
author_role author
author2 Rivera Pérez, Crisalejandra
Diambra, Luis Anibal
Noriega, Fernando Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv JUVENILE HORMONE SYNTHESIS
MODELING BIOSYNTHESIS
AEDES AEGYPTI
MEVALONIC ACID PATHWAY
topic JUVENILE HORMONE SYNTHESIS
MODELING BIOSYNTHESIS
AEDES AEGYPTI
MEVALONIC ACID PATHWAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Juvenile hormone (JH) regulates development and reproductive maturation in insects. Thecorpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, whichhave been linked to the ovarian development and are influenced by nutritional signals. Therate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, whichis the outcome of a complex interplay of changes in precursor pools and enzyme levels. Acomprehensive study of the changes in enzymatic activities and precursor pool sizes havebeen previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In thepresent studies, we used two different quantitative approaches to describe and predict howchanges in the individual metabolic reactions in the pathway affect JH synthesis. First, weconstructed generalized additive models (GAMs) that described the association betweenchanges in specific metabolite concentrations with changes in enzymatic activities and sub-strate concentrations. Changes in substrate concentrations explained 50% or more of themodel deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzy-matic activities almost always improved the fitness of GAMs built solely based on substrateconcentrations. GAMs were validated using experimental data that were not included whenthe model was built. In addition, a system of ordinary differential equations (ODE) wasdeveloped to describe the instantaneous changes in metabolites as a function of the levelsof enzymatic catalytic activities. The results demonstrated the ability of the models to predictchanges in the flux of metabolites in the JH pathway, and can be used in the future to designand validate experimental manipulations of JH synthesis.
Fil: Martínez Rincón, Raúl O.. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
Fil: Rivera Pérez, Crisalejandra. Consejo Nacional de Ciencia y Tecnología; México. Centro de Investigaciones Biológicas del Noroeste; México
Fil: Diambra, Luis Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Noriega, Fernando Gabriel. Florida International University; Estados Unidos
description Juvenile hormone (JH) regulates development and reproductive maturation in insects. Thecorpora allata (CA) from female adult mosquitoes synthesize fluctuating levels of JH, whichhave been linked to the ovarian development and are influenced by nutritional signals. Therate of JH biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, whichis the outcome of a complex interplay of changes in precursor pools and enzyme levels. Acomprehensive study of the changes in enzymatic activities and precursor pool sizes havebeen previously reported for the mosquito Aedes aegypti JH biosynthesis pathway. In thepresent studies, we used two different quantitative approaches to describe and predict howchanges in the individual metabolic reactions in the pathway affect JH synthesis. First, weconstructed generalized additive models (GAMs) that described the association betweenchanges in specific metabolite concentrations with changes in enzymatic activities and sub-strate concentrations. Changes in substrate concentrations explained 50% or more of themodel deviances in 7 of the 13 metabolic steps analyzed. Addition of information on enzy-matic activities almost always improved the fitness of GAMs built solely based on substrateconcentrations. GAMs were validated using experimental data that were not included whenthe model was built. In addition, a system of ordinary differential equations (ODE) wasdeveloped to describe the instantaneous changes in metabolites as a function of the levelsof enzymatic catalytic activities. The results demonstrated the ability of the models to predictchanges in the flux of metabolites in the JH pathway, and can be used in the future to designand validate experimental manipulations of JH synthesis.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78966
Martínez Rincón, Raúl O.; Rivera Pérez, Crisalejandra; Diambra, Luis Anibal; Noriega, Fernando Gabriel; Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations; Public Library of Science; Plos One; 12; 2; 3-2-2017; 1-21
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78966
identifier_str_mv Martínez Rincón, Raúl O.; Rivera Pérez, Crisalejandra; Diambra, Luis Anibal; Noriega, Fernando Gabriel; Modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations; Public Library of Science; Plos One; 12; 2; 3-2-2017; 1-21
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1371/journal.pone.0171516
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171516
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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