Use of a new combination of xylazine-midazolam for rats sedation

Autores
Zeni Coronel, Estefania Magali; Bonanno, Marina Soledad; Seijo, Mariana; Zeni, Susana Noemi
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
One of the most used and routinely established anesthetic modalities in rodents, is ketamine (K) combined with benzodiazepines; however, K induces secondary undesired effects, added to that its acquisition in Argentina is difficult due to being considered an abuse drug. To avoid these adverse, bureaucratic and economic obstacles, we evaluated the possibility of replacing ketamine/xylazine (KX) by an anesthesia protocol that could provide a quick and effective surgical level of anesthesia and allow full access to the oral cavity. Wistar rats (n=24) were subject to the application of saline solution 3 times a week and weekly replacement of the periodontal ligature (PL) under a combination of 20 mg X/Kg and 5 mg midazolam (M)/Kg. After 21 days, animals were sacrificed and soft organs (liver, kidneys and duodenum) were extracted for histology and pharmacological combination safety verification. The time for sedation (in seconds) was evaluated and compared against the KX combination. Results: average ± SD (min-max): ataxia 113±60 (62-325); lateral decubitus 164±62 (76-359); foot reflex 306±112 (129-537) and ocular reflex 527±215 (145-1063). Mortality and respiratory distress were not observed. Ataxia marked the beginning of the induction period and was obtained after less than 2±1 min compared to 8.8±4.0 min with KX. After the completion of the dental procedure with XM, foot reflex recovery required 38±14 min and ocular reflex 43±19 min. Sedation lasted: 77±10min versus 20-40 min induced by intramuscular/intraperitoneal K/X (40-90 mg/kg/5-15mg/kg, respectively). Variability in depth of anesthesia was not observed in XM as compared to the marked variability observed with KX combination. Conclusion: Induction to the anesthetic plane was significantly shorter with XM than KX combination. The tested combination appears to be a suitable alternative to replace KX for minor oral procedures, as well as for longer surgical interventions due to its prolonged anesthesia effect.
Fil: Zeni Coronel, Estefania Magali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Seijo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
XYLAZINE
MIDAZOLAM
ANESTHESIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223541
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Use of a new combination of xylazine-midazolam for rats sedationZeni Coronel, Estefania MagaliBonanno, Marina SoledadSeijo, MarianaZeni, Susana NoemiXYLAZINEMIDAZOLAMANESTHESIAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4One of the most used and routinely established anesthetic modalities in rodents, is ketamine (K) combined with benzodiazepines; however, K induces secondary undesired effects, added to that its acquisition in Argentina is difficult due to being considered an abuse drug. To avoid these adverse, bureaucratic and economic obstacles, we evaluated the possibility of replacing ketamine/xylazine (KX) by an anesthesia protocol that could provide a quick and effective surgical level of anesthesia and allow full access to the oral cavity. Wistar rats (n=24) were subject to the application of saline solution 3 times a week and weekly replacement of the periodontal ligature (PL) under a combination of 20 mg X/Kg and 5 mg midazolam (M)/Kg. After 21 days, animals were sacrificed and soft organs (liver, kidneys and duodenum) were extracted for histology and pharmacological combination safety verification. The time for sedation (in seconds) was evaluated and compared against the KX combination. Results: average ± SD (min-max): ataxia 113±60 (62-325); lateral decubitus 164±62 (76-359); foot reflex 306±112 (129-537) and ocular reflex 527±215 (145-1063). Mortality and respiratory distress were not observed. Ataxia marked the beginning of the induction period and was obtained after less than 2±1 min compared to 8.8±4.0 min with KX. After the completion of the dental procedure with XM, foot reflex recovery required 38±14 min and ocular reflex 43±19 min. Sedation lasted: 77±10min versus 20-40 min induced by intramuscular/intraperitoneal K/X (40-90 mg/kg/5-15mg/kg, respectively). Variability in depth of anesthesia was not observed in XM as compared to the marked variability observed with KX combination. Conclusion: Induction to the anesthetic plane was significantly shorter with XM than KX combination. The tested combination appears to be a suitable alternative to replace KX for minor oral procedures, as well as for longer surgical interventions due to its prolonged anesthesia effect.Fil: Zeni Coronel, Estefania Magali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; ArgentinaFil: Seijo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223541Use of a new combination of xylazine-midazolam for rats sedation; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 1-51669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/36368022.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:10Zoai:ri.conicet.gov.ar:11336/223541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:11.214CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of a new combination of xylazine-midazolam for rats sedation
title Use of a new combination of xylazine-midazolam for rats sedation
spellingShingle Use of a new combination of xylazine-midazolam for rats sedation
Zeni Coronel, Estefania Magali
XYLAZINE
MIDAZOLAM
ANESTHESIA
title_short Use of a new combination of xylazine-midazolam for rats sedation
title_full Use of a new combination of xylazine-midazolam for rats sedation
title_fullStr Use of a new combination of xylazine-midazolam for rats sedation
title_full_unstemmed Use of a new combination of xylazine-midazolam for rats sedation
title_sort Use of a new combination of xylazine-midazolam for rats sedation
dc.creator.none.fl_str_mv Zeni Coronel, Estefania Magali
Bonanno, Marina Soledad
Seijo, Mariana
Zeni, Susana Noemi
author Zeni Coronel, Estefania Magali
author_facet Zeni Coronel, Estefania Magali
Bonanno, Marina Soledad
Seijo, Mariana
Zeni, Susana Noemi
author_role author
author2 Bonanno, Marina Soledad
Seijo, Mariana
Zeni, Susana Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv XYLAZINE
MIDAZOLAM
ANESTHESIA
topic XYLAZINE
MIDAZOLAM
ANESTHESIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv One of the most used and routinely established anesthetic modalities in rodents, is ketamine (K) combined with benzodiazepines; however, K induces secondary undesired effects, added to that its acquisition in Argentina is difficult due to being considered an abuse drug. To avoid these adverse, bureaucratic and economic obstacles, we evaluated the possibility of replacing ketamine/xylazine (KX) by an anesthesia protocol that could provide a quick and effective surgical level of anesthesia and allow full access to the oral cavity. Wistar rats (n=24) were subject to the application of saline solution 3 times a week and weekly replacement of the periodontal ligature (PL) under a combination of 20 mg X/Kg and 5 mg midazolam (M)/Kg. After 21 days, animals were sacrificed and soft organs (liver, kidneys and duodenum) were extracted for histology and pharmacological combination safety verification. The time for sedation (in seconds) was evaluated and compared against the KX combination. Results: average ± SD (min-max): ataxia 113±60 (62-325); lateral decubitus 164±62 (76-359); foot reflex 306±112 (129-537) and ocular reflex 527±215 (145-1063). Mortality and respiratory distress were not observed. Ataxia marked the beginning of the induction period and was obtained after less than 2±1 min compared to 8.8±4.0 min with KX. After the completion of the dental procedure with XM, foot reflex recovery required 38±14 min and ocular reflex 43±19 min. Sedation lasted: 77±10min versus 20-40 min induced by intramuscular/intraperitoneal K/X (40-90 mg/kg/5-15mg/kg, respectively). Variability in depth of anesthesia was not observed in XM as compared to the marked variability observed with KX combination. Conclusion: Induction to the anesthetic plane was significantly shorter with XM than KX combination. The tested combination appears to be a suitable alternative to replace KX for minor oral procedures, as well as for longer surgical interventions due to its prolonged anesthesia effect.
Fil: Zeni Coronel, Estefania Magali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Bonanno, Marina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Seijo, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description One of the most used and routinely established anesthetic modalities in rodents, is ketamine (K) combined with benzodiazepines; however, K induces secondary undesired effects, added to that its acquisition in Argentina is difficult due to being considered an abuse drug. To avoid these adverse, bureaucratic and economic obstacles, we evaluated the possibility of replacing ketamine/xylazine (KX) by an anesthesia protocol that could provide a quick and effective surgical level of anesthesia and allow full access to the oral cavity. Wistar rats (n=24) were subject to the application of saline solution 3 times a week and weekly replacement of the periodontal ligature (PL) under a combination of 20 mg X/Kg and 5 mg midazolam (M)/Kg. After 21 days, animals were sacrificed and soft organs (liver, kidneys and duodenum) were extracted for histology and pharmacological combination safety verification. The time for sedation (in seconds) was evaluated and compared against the KX combination. Results: average ± SD (min-max): ataxia 113±60 (62-325); lateral decubitus 164±62 (76-359); foot reflex 306±112 (129-537) and ocular reflex 527±215 (145-1063). Mortality and respiratory distress were not observed. Ataxia marked the beginning of the induction period and was obtained after less than 2±1 min compared to 8.8±4.0 min with KX. After the completion of the dental procedure with XM, foot reflex recovery required 38±14 min and ocular reflex 43±19 min. Sedation lasted: 77±10min versus 20-40 min induced by intramuscular/intraperitoneal K/X (40-90 mg/kg/5-15mg/kg, respectively). Variability in depth of anesthesia was not observed in XM as compared to the marked variability observed with KX combination. Conclusion: Induction to the anesthetic plane was significantly shorter with XM than KX combination. The tested combination appears to be a suitable alternative to replace KX for minor oral procedures, as well as for longer surgical interventions due to its prolonged anesthesia effect.
publishDate 2022
dc.date.none.fl_str_mv 2022
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info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223541
Use of a new combination of xylazine-midazolam for rats sedation; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 1-5
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223541
identifier_str_mv Use of a new combination of xylazine-midazolam for rats sedation; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 1-5
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/36368022.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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