Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs

Autores
Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhanrd, Victoria; Heredia, Valeria; Beltramo, Dante Miguel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recently, we demonstrated that GM1 micelles transport paclitaxel and doxorubicin with high efficiency. Whenthis GM1-drugs complex is incubated with whole serum, albumin was the only one protein that binds spontaneouslyto form GM1-drug-albumin complex. Here, we show that, under specific physicochemical conditions, these micellesinteract with antibodies forming GM1-IgG complexes. The load of IgG in GM1 reaches a maximum at ratios of 1/4(w / w) incubating to 4.5 and preheating the micelles of GM1 at 55-60°C. The IgG of the GM1-IgG complex obtainedunder these experimental conditions retains the biological activity against the soluble and cellular antigens and is notdisplaced from the micelles in the presence of albumin, the main competitive binding protein.Treatment of GM1-IgG with pepsin, does not show the breakage of the IgG like control of free IgG, suggesting thatIgG is deeply bound into GM1, probably via Fc. Moreover, the presence of 1 M NaCl does not prevent neither dissociatethe complex, suggesting the hydrophobic nature of the interaction. The DLS and TEM results shows that GM1-IgGcomplexes have sizes significantly higher than those of GM1 micelles; this is directly related to the amount of IgGloaded. On the other hand GM1-IgG complex also retain the ability to encapsulate oncological drugs, but, an adequatesequence must be followed during the preparation, in order to obtain efficient GM1-drug-IgG ternary complexes. Moreover, the presence of IgG into GM1-oncological drugs complex do not affect the release or the citotoxic activity ofthe encapsulated molecules such as Ptx or Doxo.
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonhanrd, Victoria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Heredia, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beltramo, Dante Miguel. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
INMUNOMICELLES
MONOSIALOGANGLIOSIDOS (GM1)
TARGETED DRUG DELIVERY
NANOMEDICINE
CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106547
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/106547
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugsGarro, Ariel GustavoAlasino, Roxana ValeriaLeonhanrd, VictoriaHeredia, ValeriaBeltramo, Dante MiguelINMUNOMICELLESMONOSIALOGANGLIOSIDOS (GM1)TARGETED DRUG DELIVERYNANOMEDICINECANCERhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Recently, we demonstrated that GM1 micelles transport paclitaxel and doxorubicin with high efficiency. Whenthis GM1-drugs complex is incubated with whole serum, albumin was the only one protein that binds spontaneouslyto form GM1-drug-albumin complex. Here, we show that, under specific physicochemical conditions, these micellesinteract with antibodies forming GM1-IgG complexes. The load of IgG in GM1 reaches a maximum at ratios of 1/4(w / w) incubating to 4.5 and preheating the micelles of GM1 at 55-60°C. The IgG of the GM1-IgG complex obtainedunder these experimental conditions retains the biological activity against the soluble and cellular antigens and is notdisplaced from the micelles in the presence of albumin, the main competitive binding protein.Treatment of GM1-IgG with pepsin, does not show the breakage of the IgG like control of free IgG, suggesting thatIgG is deeply bound into GM1, probably via Fc. Moreover, the presence of 1 M NaCl does not prevent neither dissociatethe complex, suggesting the hydrophobic nature of the interaction. The DLS and TEM results shows that GM1-IgGcomplexes have sizes significantly higher than those of GM1 micelles; this is directly related to the amount of IgGloaded. On the other hand GM1-IgG complex also retain the ability to encapsulate oncological drugs, but, an adequatesequence must be followed during the preparation, in order to obtain efficient GM1-drug-IgG ternary complexes. Moreover, the presence of IgG into GM1-oncological drugs complex do not affect the release or the citotoxic activity ofthe encapsulated molecules such as Ptx or Doxo.Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leonhanrd, Victoria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Heredia, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Beltramo, Dante Miguel. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLongdom2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106547Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhanrd, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs; Longdom; J Nanomed Nanotechnol; 10; 3; 5-20192157-7439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7439.1000532info:eu-repo/semantics/altIdentifier/url/https://www.longdom.org/abstract/antibodies-can-be-spontaneously-loaded-onto-monosialoganglioside-micelles-containing-oncological-drugs-37543.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:12Zoai:ri.conicet.gov.ar:11336/106547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:12.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
title Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
spellingShingle Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
Garro, Ariel Gustavo
INMUNOMICELLES
MONOSIALOGANGLIOSIDOS (GM1)
TARGETED DRUG DELIVERY
NANOMEDICINE
CANCER
title_short Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
title_full Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
title_fullStr Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
title_full_unstemmed Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
title_sort Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs
dc.creator.none.fl_str_mv Garro, Ariel Gustavo
Alasino, Roxana Valeria
Leonhanrd, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author Garro, Ariel Gustavo
author_facet Garro, Ariel Gustavo
Alasino, Roxana Valeria
Leonhanrd, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author_role author
author2 Alasino, Roxana Valeria
Leonhanrd, Victoria
Heredia, Valeria
Beltramo, Dante Miguel
author2_role author
author
author
author
dc.subject.none.fl_str_mv INMUNOMICELLES
MONOSIALOGANGLIOSIDOS (GM1)
TARGETED DRUG DELIVERY
NANOMEDICINE
CANCER
topic INMUNOMICELLES
MONOSIALOGANGLIOSIDOS (GM1)
TARGETED DRUG DELIVERY
NANOMEDICINE
CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recently, we demonstrated that GM1 micelles transport paclitaxel and doxorubicin with high efficiency. Whenthis GM1-drugs complex is incubated with whole serum, albumin was the only one protein that binds spontaneouslyto form GM1-drug-albumin complex. Here, we show that, under specific physicochemical conditions, these micellesinteract with antibodies forming GM1-IgG complexes. The load of IgG in GM1 reaches a maximum at ratios of 1/4(w / w) incubating to 4.5 and preheating the micelles of GM1 at 55-60°C. The IgG of the GM1-IgG complex obtainedunder these experimental conditions retains the biological activity against the soluble and cellular antigens and is notdisplaced from the micelles in the presence of albumin, the main competitive binding protein.Treatment of GM1-IgG with pepsin, does not show the breakage of the IgG like control of free IgG, suggesting thatIgG is deeply bound into GM1, probably via Fc. Moreover, the presence of 1 M NaCl does not prevent neither dissociatethe complex, suggesting the hydrophobic nature of the interaction. The DLS and TEM results shows that GM1-IgGcomplexes have sizes significantly higher than those of GM1 micelles; this is directly related to the amount of IgGloaded. On the other hand GM1-IgG complex also retain the ability to encapsulate oncological drugs, but, an adequatesequence must be followed during the preparation, in order to obtain efficient GM1-drug-IgG ternary complexes. Moreover, the presence of IgG into GM1-oncological drugs complex do not affect the release or the citotoxic activity ofthe encapsulated molecules such as Ptx or Doxo.
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonhanrd, Victoria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Heredia, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beltramo, Dante Miguel. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Recently, we demonstrated that GM1 micelles transport paclitaxel and doxorubicin with high efficiency. Whenthis GM1-drugs complex is incubated with whole serum, albumin was the only one protein that binds spontaneouslyto form GM1-drug-albumin complex. Here, we show that, under specific physicochemical conditions, these micellesinteract with antibodies forming GM1-IgG complexes. The load of IgG in GM1 reaches a maximum at ratios of 1/4(w / w) incubating to 4.5 and preheating the micelles of GM1 at 55-60°C. The IgG of the GM1-IgG complex obtainedunder these experimental conditions retains the biological activity against the soluble and cellular antigens and is notdisplaced from the micelles in the presence of albumin, the main competitive binding protein.Treatment of GM1-IgG with pepsin, does not show the breakage of the IgG like control of free IgG, suggesting thatIgG is deeply bound into GM1, probably via Fc. Moreover, the presence of 1 M NaCl does not prevent neither dissociatethe complex, suggesting the hydrophobic nature of the interaction. The DLS and TEM results shows that GM1-IgGcomplexes have sizes significantly higher than those of GM1 micelles; this is directly related to the amount of IgGloaded. On the other hand GM1-IgG complex also retain the ability to encapsulate oncological drugs, but, an adequatesequence must be followed during the preparation, in order to obtain efficient GM1-drug-IgG ternary complexes. Moreover, the presence of IgG into GM1-oncological drugs complex do not affect the release or the citotoxic activity ofthe encapsulated molecules such as Ptx or Doxo.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106547
Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhanrd, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs; Longdom; J Nanomed Nanotechnol; 10; 3; 5-2019
2157-7439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106547
identifier_str_mv Garro, Ariel Gustavo; Alasino, Roxana Valeria; Leonhanrd, Victoria; Heredia, Valeria; Beltramo, Dante Miguel; Antibodies can be spontaneously loaded onto monosialoganglioside micelles containing oncological drugs; Longdom; J Nanomed Nanotechnol; 10; 3; 5-2019
2157-7439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7439.1000532
info:eu-repo/semantics/altIdentifier/url/https://www.longdom.org/abstract/antibodies-can-be-spontaneously-loaded-onto-monosialoganglioside-micelles-containing-oncological-drugs-37543.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Longdom
publisher.none.fl_str_mv Longdom
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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